PAM-flexible genome editing with an engineered chimeric Cas9.

Zhao L, Koseki SRT, Silverstein RA, Amrani N, Peng C, Kramme C, Savic N, Pacesa M, Rodriguez TC, Stan T, Tysinger E, Hong L, Yudistyra V, Ponnapati MR, Jacobson JM, Church GM, Jakimo N, Truant R, Jinek M, Kleinstiver BP, Sontheimer EJ, Chatterjee P
Nat Commun. 2023 Oct 4;14(1):6175. doi: 10.1038/s41467-023-41829-y. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
208336	pCAG-CBE4max-SpRYc-P2A-EGFP	CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with SpRYc
208340	pCMV-SpRYc-ABE8e-EGFP	Expresses SpRYc-ABE8e in mammalian cells