pCAG-CBE4max-SpRYc-P2A-EGFP
(Plasmid #208336)

• Purpose: CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with SpRYc
• Depositing Lab: Pranam Chatterjee
• Publication: Zhao et al Nat Commun. 2023 Oct 4;14(1):6175. doi: 10.1038/s41467-023-41829-y.

Backbone

• Vector backbone: pRZ33
• Backbone manufacturer: Keith Joung
• Total vector size (bp): 11222
• Vector type: Mammalian Expression, CRISPR

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: human codon optimized CBE4max Cas9 variant named SpRYc with P2A-EGFP
• Species: Synthetic
• Insert Size (bp): 6380
• Mutation: Sc++ (1-1119), SpRY (1111-1368), D10A
• Promoter: CAG
• Tag / Fusion Protein:
  • P2A-EGFP (C terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: CMV-Forward
• 3′ sequencing primer: EGFP-N

Resource Information

• Supplemental Documents:
  • pCAG-CBE4Max-SpRYc-EGFP.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that the sequence IDs for Plasmid 208336: pCAG-CBE4max-SpRYc-P2A-EGFP and Plasmid 208340: pCMV-SpRYc-ABE8e are incorrectly swapped in the associated paper. The correct plasmid ID for pCAG-CBE4max-SpRYc-P2A-EGFP is 208336.