The guide-RNA sequence dictates the slicing kinetics and conformational dynamics of the Argonaute silencing complex.
Wang PY, Bartel DP
Mol Cell. 2024 Jul 10:S1097-2765(24)00533-1. doi: 10.1016/j.molcel.2024.06.026.
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PubMed
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 220461 | pcDNA3.3−3xFLAG−AGO2(D669A) | Express catalytically-dead mutant human AGO2 with N-terminal 3xFLAG tag in mammalian cells |
| 220462 | pcDNA3.3−3xFLAG−AGO2(S387A) | Express S387 phospho-null mutant human AGO2 with N-terminal 3xFLAG tag in mammalian cells |
| 220463 | pcDNA3.3−3xFLAG−AGO2(S387D) | Express S387 phosphomimetic mutant human AGO2 with N-terminal 3xFLAG tag in mammalian cells |
| 220464 | pSliceRep_A_miR-7 | Dual luciferase reporter for RNAi, with target site within a tunable ORF (version A) |
| 220465 | pSliceRep_A_miR-7.M4 | Dual luciferase reporter for RNAi, with target site within a tunable ORF (version A) |
| 220466 | pSliceRep_A_miR-196a | Dual luciferase reporter for RNAi, with target site within a tunable ORF (version A) |
| 220467 | pSliceRep_A_miR-196a.M3 | Dual luciferase reporter for RNAi, with target site within a tunable ORF (version A) |
| 220468 | pSliceRep_B_miR-7 | Dual luciferase reporter for RNAi, with target site within a tunable ORF (version B) |
| 220469 | pSliceRep_B_miR-7.M4 | Dual luciferase reporter for RNAi, with target site within a tunable ORF (version B) |
| 220470 | pSliceRep_B_miR-196a | Dual luciferase reporter for RNAi, with target site within a tunable ORF (version B) |
| 220471 | pSliceRep_B_miR-196a.M3 | Dual luciferase reporter for RNAi, with target site within a tunable ORF (version B) |