Application of Escherichia coli phage K1E DNA-dependent RNA polymerase for in vitro RNA synthesis and in vivo protein production in Bacillus megaterium.

Stammen S, Schuller F, Dietrich S, Gamer M, Biedendieck R, Jahn D
Appl Microbiol Biotechnol. 2010 Sep;88(2):529-39. doi: 10.1007/s00253-010-2732-y. Epub 2010 Jul 2. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
48130	pPSP6+t-gfp	Shuttle vector E. coli/B.meg.; gfp-expression under control of RNAP-SP6-inducible promoter
48131	pPK1E-1+t-gfp	Shuttle vector E. coli/B.meg.; gfp-expression under control of RNAP-K1E-inducible promoter
48132	pPK1E-2+t-gfp	Shuttle vector E. coli/B.meg.; gfp-expression under control of RNAP-K1E-inducible promoter
48133	pPK1E-1-gfp	Shuttle vector E. coli/B.meg.; gfp-expression under control of RNAP-K1E-inducible promoter
48143	pSP6-RNAP	Shuttle vector E. coli/B.meg.; encoding the RNA polymerase of the phage SP6 under control of the xylose inducible promoter PxylA
48144	pK1E-RNAP	Shuttle vector E. coli/B.meg.; encoding the RNA polymerase of the phage K1E under control of the xylose inducible promoter PxylA
48145	pPK1E-1	Shuttle vector E. coli/B.meg., encoding a promoter for RNA polymerase of the phage K1E
48146	pPK1E-2	Shuttle vector E. coli/B.meg., encoding a promoter for RNA polymerase of the phage K1E
48147	pPK1E-1+t	Shuttle vector E. coli/B.meg., encoding a promoter and a terminator for RNA polymerase of the phage K1E
48148	pPK1E-2+t	Shuttle vector E. coli/B.meg., encoding a promoter and a terminator for RNA polymerase of the phage K1E
48149	pPSP6+t	Shuttle vector E. coli/B.meg., encoding a promoter and a terminator for RNA polymerase of the phage SP6
48150	pPSP6	Shuttle vector E. coli/B.meg., encoding a promoter for RNA polymerase of the phage SP6