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pSP6-RNAP
(Plasmid #48143)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 48143 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pMGBm19
  • Backbone manufacturer
    Gamer et al., 2009
  • Vector type
    Bacterial Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH10B
  • Growth instructions
    For Bacillus megaterium: Chloramphenicol and 37 °C
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    rna polymerase SP6

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSP6-RNAP was a gift from Dieter Jahn (Addgene plasmid # 48143 ; http://n2t.net/addgene:48143 ; RRID:Addgene_48143)
  • For your References section:

    Application of Escherichia coli phage K1E DNA-dependent RNA polymerase for in vitro RNA synthesis and in vivo protein production in Bacillus megaterium. Stammen S, Schuller F, Dietrich S, Gamer M, Biedendieck R, Jahn D. Appl Microbiol Biotechnol. 2010 Sep;88(2):529-39. doi: 10.1007/s00253-010-2732-y. Epub 2010 Jul 2. 10.1007/s00253-010-2732-y PubMed 20596705