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pTARA
(Plasmid #31491)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 31491 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol
  • Growth Temperature
    30°C
  • Growth Strain(s)
    GC5
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    RNA polymerase
  • Alt name
    T7 RNA polymerase
  • Species
    T7 phage
  • Insert Size (bp)
    2652
  • Mutation
    N823D

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Sac1 (not destroyed)
  • 3′ cloning site Xba1 (not destroyed)
  • 5′ sequencing primer ATGCCATAGCATTTTTATCC
  • 3′ sequencing primer GATTTAATCTGTATCAGG
  • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    pAR1219 encoded T7 polymerase, obtained from Ian Molineux, University of Texas; pBAD33 was obtained from L. Guzman
  • Terms and Licenses
  • Articles Citing this Plasmid

Depositor Comments

For expression: E. coli CV2

N823D mutation should not affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTARA was a gift from Kathleen Matthews (Addgene plasmid # 31491 ; http://n2t.net/addgene:31491 ; RRID:Addgene_31491)
  • For your References section:

    Generation of an AraC-araBAD promoter-regulated T7 expression system. Wycuff DR, Matthews KS. Anal Biochem. 2000 Jan 1. 277(1):67-73. 10.1006/abio.1999.4385 PubMed 10610690