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pET22b RNase A
(Plasmid #58903)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 58903 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pET22b(+)
  • Backbone manufacturer
    EMD Millipore
  • Backbone size w/o insert (bp) 5493
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    RNase A
  • Species
    B. taurus (bovine)
  • Insert Size (bp)
    500
  • Mutation
    See Depositor Comments below
  • GenBank ID
    NP_001014408
  • Entrez Gene
    RNASE1 (a.k.a. RNS1)
  • Promoter T7
  • Tag / Fusion Protein
    • pelB sequence (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (destroyed during cloning)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7-Term
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note that the pelB sequence is maintained however there are stop codons incorporated at the end of the gene so the His-Tag is not in frame. The pelB is clipped off and the gene encodes 124 amino acid protein that is expressed/purified from inclusion bodies.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET22b RNase A was a gift from Ronald Raines (Addgene plasmid # 58903 ; http://n2t.net/addgene:58903 ; RRID:Addgene_58903)
  • For your References section:

    Engineering ribonuclease A: production, purification and characterization of wild-type enzyme and mutants at Gln11. delCardayre SB, Ribo M, Yokel EM, Quirk DJ, Rutter WJ, Raines RT. Protein Eng. 1995 Mar;8(3):261-73. PubMed 7479688