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pCS6
(Plasmid #55752)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 55752 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH10B
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    T7 RNA Polymerase
  • Species
    Escherichia coli
  • Insert Size (bp)
    2652
  • Promoter pBAD

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer GCCCTGCTGCGTAACATCGTT
  • 3′ sequencing primer GCGATGCTGCTTATCGAATCAAAGCT
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Used pTara's (Dr. Kathleen Matthews) T7 RNAP and AraC.
  • Terms and Licenses
  • Article Citing this Plasmid
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCS6 was a gift from Matthew Bennett (Addgene plasmid # 55752 ; http://n2t.net/addgene:55752 ; RRID:Addgene_55752)
  • For your References section:

    Stable maintenance of multiple plasmids in E. coli using a single selective marker. Schmidt CM, Shis DL, Nguyen-Huu TD, Bennett MR. ACS Synth Biol. 2012 Oct 19;1(10):445-50. doi: 10.1021/sb3000589. Epub 2012 Sep 4. 10.1021/sb3000589 PubMed 23656183