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Application of Escherichia coli phage K1E DNA-dependent RNA polymerase for in vitro RNA synthesis and in vivo protein production in Bacillus megaterium.
Stammen S, Schuller F, Dietrich S, Gamer M, Biedendieck R, Jahn D
Appl Microbiol Biotechnol. 2010 Sep;88(2):529-39. doi: 10.1007/s00253-010-2732-y. Epub 2010 Jul 2.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
48130pPSP6+t-gfpShuttle vector E. coli/B.meg.; gfp-expression under control of RNAP-SP6-inducible promoter
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48131pPK1E-1+t-gfpShuttle vector E. coli/B.meg.; gfp-expression under control of RNAP-K1E-inducible promoter
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48132pPK1E-2+t-gfpShuttle vector E. coli/B.meg.; gfp-expression under control of RNAP-K1E-inducible promoter
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48133pPK1E-1-gfpShuttle vector E. coli/B.meg.; gfp-expression under control of RNAP-K1E-inducible promoter
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48143pSP6-RNAPShuttle vector E. coli/B.meg.; encoding the RNA polymerase of the phage SP6 under control of the xylose inducible promoter PxylA
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48144pK1E-RNAPShuttle vector E. coli/B.meg.; encoding the RNA polymerase of the phage K1E under control of the xylose inducible promoter PxylA
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48145pPK1E-1Shuttle vector E. coli/B.meg., encoding a promoter for RNA polymerase of the phage K1E
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48146pPK1E-2Shuttle vector E. coli/B.meg., encoding a promoter for RNA polymerase of the phage K1E
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48147pPK1E-1+tShuttle vector E. coli/B.meg., encoding a promoter and a terminator for RNA polymerase of the phage K1E
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48148pPK1E-2+tShuttle vector E. coli/B.meg., encoding a promoter and a terminator for RNA polymerase of the phage K1E
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48149pPSP6+tShuttle vector E. coli/B.meg., encoding a promoter and a terminator for RNA polymerase of the phage SP6
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48150pPSP6Shuttle vector E. coli/B.meg., encoding a promoter for RNA polymerase of the phage SP6
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