Application of Escherichia coli phage K1E DNA-dependent RNA polymerase for in vitro RNA synthesis and in vivo protein production in Bacillus megaterium.
Stammen S, Schuller F, Dietrich S, Gamer M, Biedendieck R, Jahn D
Appl Microbiol Biotechnol. 2010 Sep;88(2):529-39. doi: 10.1007/s00253-010-2732-y. Epub 2010 Jul 2. PubMed Article
Stammen S, Schuller F, Dietrich S, Gamer M, Biedendieck R, Jahn D
Appl Microbiol Biotechnol. 2010 Sep;88(2):529-39. doi: 10.1007/s00253-010-2732-y. Epub 2010 Jul 2. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 48130 | pPSP6+t-gfp | Shuttle vector E. coli/B.meg.; gfp-expression under control of RNAP-SP6-inducible promoter |
| 48131 | pPK1E-1+t-gfp | Shuttle vector E. coli/B.meg.; gfp-expression under control of RNAP-K1E-inducible promoter |
| 48132 | pPK1E-2+t-gfp | Shuttle vector E. coli/B.meg.; gfp-expression under control of RNAP-K1E-inducible promoter |
| 48133 | pPK1E-1-gfp | Shuttle vector E. coli/B.meg.; gfp-expression under control of RNAP-K1E-inducible promoter |
| 48143 | pSP6-RNAP | Shuttle vector E. coli/B.meg.; encoding the RNA polymerase of the phage SP6 under control of the xylose inducible promoter PxylA |
| 48144 | pK1E-RNAP | Shuttle vector E. coli/B.meg.; encoding the RNA polymerase of the phage K1E under control of the xylose inducible promoter PxylA |
| 48145 | pPK1E-1 | Shuttle vector E. coli/B.meg., encoding a promoter for RNA polymerase of the phage K1E |
| 48146 | pPK1E-2 | Shuttle vector E. coli/B.meg., encoding a promoter for RNA polymerase of the phage K1E |
| 48147 | pPK1E-1+t | Shuttle vector E. coli/B.meg., encoding a promoter and a terminator for RNA polymerase of the phage K1E |
| 48148 | pPK1E-2+t | Shuttle vector E. coli/B.meg., encoding a promoter and a terminator for RNA polymerase of the phage K1E |
| 48149 | pPSP6+t | Shuttle vector E. coli/B.meg., encoding a promoter and a terminator for RNA polymerase of the phage SP6 |
| 48150 | pPSP6 | Shuttle vector E. coli/B.meg., encoding a promoter for RNA polymerase of the phage SP6 |