Allosteric inhibition of a stem cell RNA-binding protein by an intermediary metabolite.
Clingman CC, Deveau LM, Hay SA, Genga RM, Shandilya SM, Massi F, Ryder SP
Elife. 2014 Jun 16:e02848. doi: 10.7554/eLife.02848.
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 60245 | pET-22HT-MSI1(7-192) | Express the RBD of mouse MSI1 in bacterial cells for protein purification |
| 60346 | pET-22HT-MSI1(7-192)Q30D | Q30D mutant of the mouse MSI1 RBD |
| 60347 | pET-22HT-MSI1(7-192)G35D | G35D mutant of the mouse MSI1 RBD |
| 60348 | pET-22HT-MSI1(7-192)G35E | G35E mutant of the mouse MSI1 RBD |
| 60349 | pET-22HT-MSI1(7-192)H83F | H83F mutant of the mouse MSI1 RBD |
| 60350 | pET-22HT-MSI1(7-192)H83L | H83L mutant of the mouse MSI1 RBD |
| 60351 | pET-22HT-MSI1(7-192)G64A | G64A mutant of the mouse MSI1 RBD |
| 60352 | pET-22HT-MSI1(7-192)R53E | R53E mutant of the mouse MSI1 RBD |
| 60353 | pET-22HT-MSI1(7-192)R61E | R61E mutant of the mouse MSI1 RBD |
| 60354 | pET-22HT-MSI1(7-192)R53E/R61E | R53E R61E double mutant of the mouse MSI1 RBD |
| 60355 | pET-22HT-MSI1(7-192)R53E/R59E/R61E | R53E R59E R61E triple mutant of the mouse MSI1 RBD |
| 60356 | pET-22HT-MSI2(8-193) | Express the RBD of human MSI2 in bacterial cells for protein purification |
| 60357 | pET-22HT-MSI1(7-103) | Express the RRM1 of mouse MSI1 in bacterial cells for protein purification |
| 60358 | pCDH-CMV-MSI1(FL) | express mouse MSI1 in mammalian cells |