PurposeR53E R59E R61E triple mutant of the mouse MSI1 RBD
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||60355||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5400
- Total vector size (bp) 6000
Modifications to backbonepelB leader sequence was replaced with a His6-Gly tag followed by a TEV protease site. See pET-22HT-MSI1 (7–192) Addgene plasmid # 60245
Vector typeBacterial Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
Gene/Insert namemouse MSI1 (7-192)
SpeciesM. musculus (mouse)
Insert Size (bp)573
MutationR53E, R59E and R61E mutations; contains amino acids 7-192 of Msi1
Entrez GeneMsi1 (a.k.a. Ms, Msi, Msi1h, Musa, Musahi1, m-Msi, m-Msi-1)
- Promoter T7
/ Fusion Proteins
- His (N terminal on backbone)
- TEV protease site (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site HindIII (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer T7-term (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pET-22HT-MSI1(7-192)R53E/R59E/R61E was a gift from Sean Ryder (Addgene plasmid # 60355 ; http://n2t.net/addgene:60355 ; RRID:Addgene_60355)
For your References section:Allosteric inhibition of a stem cell RNA-binding protein by an intermediary metabolite. Clingman CC, Deveau LM, Hay SA, Genga RM, Shandilya SM, Massi F, Ryder SP. Elife. 2014 Jun 16:e02848. doi: 10.7554/eLife.02848. 10.7554/eLife.02848 PubMed 24935936
Map uploaded by the depositor.