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Allosteric inhibition of a stem cell RNA-binding protein by an intermediary metabolite.
Clingman CC, Deveau LM, Hay SA, Genga RM, Shandilya SM, Massi F, Ryder SP
Elife. 2014 Jun 16:e02848. doi: 10.7554/eLife.02848. PubMed Article

Plasmids from Article

ID Plasmid Purpose
60245pET-22HT-MSI1(7-192)Express the RBD of mouse MSI1 in bacterial cells for protein purification
60346pET-22HT-MSI1(7-192)Q30DQ30D mutant of the mouse MSI1 RBD
60347pET-22HT-MSI1(7-192)G35DG35D mutant of the mouse MSI1 RBD
60348pET-22HT-MSI1(7-192)G35EG35E mutant of the mouse MSI1 RBD
60349pET-22HT-MSI1(7-192)H83FH83F mutant of the mouse MSI1 RBD
60350pET-22HT-MSI1(7-192)H83LH83L mutant of the mouse MSI1 RBD
60351pET-22HT-MSI1(7-192)G64AG64A mutant of the mouse MSI1 RBD
60352pET-22HT-MSI1(7-192)R53ER53E mutant of the mouse MSI1 RBD
60353pET-22HT-MSI1(7-192)R61ER61E mutant of the mouse MSI1 RBD
60354pET-22HT-MSI1(7-192)R53E/R61ER53E R61E double mutant of the mouse MSI1 RBD
60355pET-22HT-MSI1(7-192)R53E/R59E/R61ER53E R59E R61E triple mutant of the mouse MSI1 RBD
60356pET-22HT-MSI2(8-193)Express the RBD of human MSI2 in bacterial cells for protein purification
60357pET-22HT-MSI1(7-103)Express the RRM1 of mouse MSI1 in bacterial cells for protein purification
60358pCDH-CMV-MSI1(FL)express mouse MSI1 in mammalian cells

Antibodies from Article