A light-inducible CRISPR-Cas9 system for control of endogenous gene activation.
Polstein LR, Gersbach CA
Nat Chem Biol. 2015 Feb 9. doi: 10.1038/nchembio.1753.
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PubMed
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 60551 | pcDNA3.1-CibN-dCas9 | Expresses CibN-dCas9 in mammalian cells |
| 60552 | pcDNA3.1-dCas9-CibN | Expresses dCas9-CibN in mammalian cells |
| 60553 | pcDNA3.1-CibN-dCas9-CibN | Expresses CibN-dCas9-CibN in mammalian cells |
| 60554 | pcDNA3.1-Cry2FL-VP64 | Expresses Cry2FL-VP64 in mammalian cells |
| 60718 | pGL3-Basic-8x-gRNA-eGFP | eGFP reporter containing 8 copies of a gRNA binding site for light-inducible dCas9 activation |
| 60719 | gRNA-eGFP-Reporter | Expresses guide RNA used to activate pGL3-Basic-8x-gRNA-eGFP reporter. |