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1208 p3e-long etv2 3’UTR

Plasmid

#49010

Purpose

Zebrafish Long length etv2 3' UTR in gateway 3' Entry vector

Depositor

Nathan Lawson

Article

Moore et al Dev Biol. 2013 Sep 11. pii: S0012-1606(1

Insert

ets variant gene 2 (etsrp Zebrafish)

Use

Gateway 3' entry

Mutation

Longest 3' UTR

Available Since

Oct. 28, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-REDD1 C140S (#622)

Plasmid

#99947

Purpose

mammalian expression

Depositor

James Brugarolas

Article

Vega-Rubin-de-Celis et al Biochemistry. 2010 Mar 23;49(11):2491-50

Insert

REDD1 (DDIT4 Human)

Tags

HA

Expression

Mammalian

Mutation

C140S

Available Since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pmalc4x_V426F_TEV

Plasmid

#47702

Purpose

Expresses MBP myocilin olfactomedin domain fusion protein with a TEV protease recognition sequence and the mutation V426F

Depositor

Raquel Lieberman

Article

Burns et al Biochemistry. 2011 Jul 5;50(26):5824-33.

Insert

Olfactomedin-like domain with V426F mutation (MYOC Human)

Tags

MBP

Expression

Bacterial

Mutation

changed V426F

Promoter

Ptac

Available Since

Sept. 9, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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pLEW100v5-BSD-T7-sgRNA

Plasmid

#245119

Purpose

Plasmid backbone to for inserting a T7 promoter driven guide RNA into the rDNA spacer of T. brucei

Depositor

Monica Mugnier

Article

Smith et al Nature. 2026 Apr 8. doi: 10.1038/s41586-

Type

Empty backbone

Use

CRISPR

Available Since

April 8, 2026

Availability

Academic Institutions and Nonprofits only

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pN1_NUP62-Fv_EGFP3

Plasmid

#252749

Purpose

Expression of NUP62 with three tandem EGFPs. FV is a modified FKBP domain (aka DmrB) whose dimerization is induced by addition of Rapalog2. Blocks nulcear pore upon addition of Rapalog2

Depositor

Kristen Verhey

Article

Takao et al Curr Biol. 2014 Oct 6;24(19):2288-94. do

Insert

NUP62 (Nup62 Mouse)

Tags

FV, 3xEGFP

Expression

Mammalian

Available Since

April 3, 2026

Availability

Industry, Academic Institutions, and Nonprofits

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pLKO.1 mTagBFP2 rat PYGB shRNA

Plasmid

#242946

Purpose

Expresses mTagBFP2 along with an shRNA against rat Glycogen Phosphorylase-B

Depositor

Timothy Ryan

Article

Pulido et al Proc Natl Acad Sci U S A. 2025 Dec 16;12

Insert

PYGB shRNA (Pygb Rat)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

U6

Available Since

Jan. 14, 2026

Availability

Academic Institutions and Nonprofits only

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pX458-SLC35A1

Plasmid

#239952

Purpose

Encodes gRNA for human SLC35A1

Depositor

Jan Carette

Article

Varanese et al Nature. 2025 Mar 25. doi: 10.1038/s41586

Insert

SLC35A1

Use

CRISPR

Expression

Mammalian

Available Since

July 24, 2025

Availability

Academic Institutions and Nonprofits only

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pX458-MFSD6

Plasmid

#239951

Purpose

Encodes gRNA for human MFSD6

Depositor

Jan Carette

Article

Varanese et al Nature. 2025 Mar 25. doi: 10.1038/s41586

Insert

MFSD6

Use

CRISPR

Expression

Mammalian

Available Since

July 24, 2025

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPRv2-ICAM-5-Puro

Plasmid

#239949

Purpose

CRISPR KO of human ICAM-5

Depositor

Jan Carette

Article

Varanese et al Nature. 2025 Mar 25. doi: 10.1038/s41586

Insert

ICAM-5

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPRv2-MFSD6-Puro

Plasmid

#239950

Purpose

CRISPR KO of human MFSD6

Depositor

Jan Carette

Article

Varanese et al Nature. 2025 Mar 25. doi: 10.1038/s41586

Insert

MFSD6

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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pIDTsmart-RC2-delVP2-VP2-454TAG

Plasmid

#240060

Purpose

Express AAV2 capsid with ncAA mutant at site 454 of VP2

Depositor

Abhishek Chatterjee

Article

Erickson et al Bioconjug Chem. 2024 Jan 17;35(1):64-71.

Inserts

RC2-DelVP2
VP2-454-TAG

Use

AAV

Expression

Mammalian

Available Since

June 30, 2025

Availability

Academic Institutions and Nonprofits only

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pIDTsmart-RC2-delVP1-VP1-454TAG

Plasmid

#240059

Purpose

Express AAV2 capsid with ncAA mutant at site 454 of VP1

Depositor

Abhishek Chatterjee

Article

Erickson et al Bioconjug Chem. 2024 Jan 17;35(1):64-71.

Inserts

RC2-DelVP1
VP1-454-TAG

Use

AAV

Expression

Mammalian

Available Since

June 30, 2025

Availability

Academic Institutions and Nonprofits only

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IMPT-11371

Plasmid

#236191

Purpose

Express GPR119 in insect cells

Depositor

Vadim Cherezov

Article

Kim et al Structure. 2024 Dec 5;32(12):2206-2219.e

Insert

GPR119 (GPR119 Human)

Expression

Insect

Promoter

polyhedrin

Available Since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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BtA3N

Plasmid

#238981

Purpose

In vitro transcription of bovine arrestin-3 short isoform

Depositor

Vsevolod Gurevich

Article

Ahmed et al Biochemistry. 2011 May 10;50(18):3749-63

Insert

arrestin-3 short (ARR3 Bovine)

Use

In vitro transcription

Mutation

silent, Cla I side introduced before stop codon

Promoter

SP6

Available Since

May 27, 2025

Availability

Academic Institutions and Nonprofits only

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pM-mCardinal

Plasmid

#228630

Purpose

Mammalian expression of mCardinal and extra insert, for MuSIC barcode construction

Depositor

Marc Birtwistle

Article

Lu et al ACS Synth Biol. 2025 May 16;14(5):1533-1

Insert

mCardinal

Expression

Mammalian

Promoter

CMV

Available Since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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pM-mPapaya

Plasmid

#228626

Purpose

Mammalian expression of mPapaya and extra insert, for MuSIC barcode construction

Depositor

Marc Birtwistle

Article

Lu et al ACS Synth Biol. 2025 May 16;14(5):1533-1

Insert

mPapaya

Expression

Mammalian

Promoter

CMV

Available Since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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pX458-MYADM

Plasmid

#235245

Purpose

Encodes gRNA for human MYADM

Depositor

Jan Carette

Article

Qiao et al Nat Commun. 2024 Apr 24;15(1):3469. doi:

Insert

MYADM (MYADM Human)

Use

CRISPR

Expression

Mammalian

Available Since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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p3A3-Flag

Plasmid

#234805

Purpose

Mammalian expression of bovine arrestin-3 with C-terminal Flag tag

Depositor

Vsevolod Gurevich

Article

Ahmed et al Biochemistry. 2011 May 10;50(18):3749-63

Insert

Arrestin-3 (ARR3 Bovine)

Tags

Flag

Expression

Mammalian

Mutation

none

Available Since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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pET-Impact-T4gp61(I342G)

Plasmid

#228208

Purpose

Expression in BL21(DE3) of T4 primase I342G

Depositor

Stephen Benkovic

Article

Valentine et al Biochemistry. 2001 Dec 18;40(50):15074-8

Insert

61 (61 Escherichia phage T4)

Tags

Sce VMA intein-chitin binding domain

Expression

Bacterial

Mutation

Mutated isoleucine 342 to glycine (I342G), improv…

Promoter

T7

Available Since

March 6, 2025

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-GST-7

Plasmid

#228989

Purpose

For bacterial expression of anti-GST nanobody GST-7, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

GST-7

Tags

6xHIS

Expression

Bacterial

Promoter

T7

Available Since

Jan. 14, 2025

Availability

Academic Institutions and Nonprofits only

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