We narrowed to 5,081 results for: biorxiv

1. GOLIM4 g1 LentiCRISPRv2-mCherry

   Plasmid

   #218662

   Purpose

   Knockout vector for human GOLIM4 (GPP130/GOLPH4)

   Depositor

   Wade Harper

   Article

   Hollingsworth et al bioRxiv 2024.04.19.590338

   Insert

   GOLIM4 (GOLIM4 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLV-SFFVp-gI-BlastR

   Plasmid

   #207179

   Purpose

   Gateway compatible Lentiviral Expression Plasmid SFFVp-IRES-BlastR

   Depositor

   David Bersten

   Article

   Allen et al bioRxiv 2023.11.21.568191

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SFFVp

   Available since

   April 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLV-SV40p-gI-BlastR

   Plasmid

   #207182

   Purpose

   Gateway compatible Lentiviral Expression Plasmid SV40p-IRES-BlastR

   Depositor

   David Bersten

   Article

   Allen et al bioRxiv 2023.11.21.568191

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1_NAP1-eGFP

   Plasmid

   #216837

   Purpose

   For mammalian expression of NAP1-GFP (Internal ref. SMC1638)

   Depositor

   Sascha Martens

   Article

   Adriaenssens et al bioRxiv September 25, 202

   Insert

   NAP1
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. Efg1-ΔDBD-Q443P

   Plasmid

   #216401

   Purpose

   Expresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with Q443 to proline mutation

   Depositor

   Nicolas Fawzi

   Article

   Wang et al bioRxiv [Preprint]. 2023 Nov 9:2023.11.0

   Insert

   Efg1-ΔDBD-Q443P
   

   Use

   Tags

   6xHis-tags

   Expression

   Bacterial

   Mutation

   changed Glutamine 443 to Proline, and deleted ami…

   Promoter

   Available since

   April 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. HA-MBP-TEVpCS-ADGRE4 CTFdeltaTA-FLAG

   Plasmid

   #217725

   Purpose

   Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

   Depositor

   Stephen Blacklow

   Article

   Dates et al bioRxiv 2022.07.14.500097

   Insert

   ADGRE4
   

   Use

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   Deletion of residues 1-179

   Promoter

   Available since

   April 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTwist Amp msTMEM115-TEV-3C-P2A-mScarlet-I HDR Template

   Plasmid

   #215000

   Purpose

   Endogenously tag mouse TMEM115 with a C-terminal 3xHA tag in addition to cytosolic mScarlet-I for cell sorting.

   Depositor

   Wade Harper

   Article

   Hollingsworth et al bioRxiv 2024.04.19.590338

   Insert

   msTMEM115-TEV-3C-P2A-mScarlet-I HDR Template (Tmem115 Mouse)
   

   Use

   Hdr template

   Tags

   3xHA

   Expression

   Mutation

   Promoter

   None

   Available since

   April 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET28a-6xHis-TrxF

   Plasmid

   #207454

   Purpose

   E. coli expression of Mesembryanthemum crystallinum Thioredoxin F with thrombin-cleavable N-terminal 6xHis tag

   Depositor

   Joseph Jez, Sarah Stainbrook

   Article

   Stainbrook et al bioRxiv 2022.08.24.505184

   Insert

   Thioredoxin F
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Codon optimized for E coli expression

   Promoter

   T7

   Available since

   Jan. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pUC57-ITR2-H1- sgCTG Bilbo -eCMV-CjSpD8A-3XFLAG-SV40 NLS-ITR2

   Plasmid

   #210749

   Purpose

   Coding for CjSp D8A alongside Bilbo sgRNA targeting CAG repeats

   Depositor

   Vincent Dion

   Article

   Mangin et al bioRxiv 2023.10.24.563270

   Inserts

   CjSp D8A Cas9
   Bilbo sgCAG
   

   Use

   CRISPR

   Tags

   3xFLAG, SV40 NLS, PolyA signal

   Expression

   Mammalian

   Mutation

   D8A, N-terminal Cj Cas9, C-terminal Sp Cas9

   Promoter

   H1 and eCMV

   Available since

   Jan. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCDH1-CMV-FIP200-V44D/V314D-mCherry-Hygro

    Plasmid

    #210855

    Purpose

    Lentiviral expression vector encoding FIP200 mutant-mCherry

    Depositor

    James Hurley

    Article

    Chen et al bioRxiv 2023.06.01.543278

    Insert

    FIP200 (RB1CC1 Human)
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mutation

    V44D/V314D

    Promoter

    Available since

    Dec. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCDH1-CMV-FIP200-V314D-mCherry-Hygro

    Plasmid

    #210854

    Purpose

    Lentiviral expression vector encoding FIP200 mutant-mCherry

    Depositor

    James Hurley

    Article

    Chen et al bioRxiv 2023.06.01.543278

    Insert

    FIP200 (RB1CC1 Human)
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mutation

    V314D

    Promoter

    Available since

    Dec. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCDH1-CMV-FIP200-V44D-mCherry-Hygro

    Plasmid

    #210853

    Purpose

    Lentiviral expression vector encoding FIP200 mutant-mCherry

    Depositor

    James Hurley

    Article

    Chen et al bioRxiv 2023.06.01.543278

    Insert

    FIP200 (RB1CC1 Human)
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mutation

    V44D

    Promoter

    Available since

    Dec. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAG-TSF-ATG101

    Plasmid

    #210858

    Purpose

    Mammalian expression of ATG101

    Depositor

    James Hurley

    Article

    Chen et al bioRxiv 2023.06.01.543278

    Insert

    ATG101 (ATG101 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAG-FIP200 (1-640)-L324D/R543D/R548D-TSF

    Plasmid

    #210842

    Purpose

    Mammalian expression of FIP200 NTD mutant

    Depositor

    James Hurley

    Article

    Chen et al bioRxiv 2023.06.01.543278

    Insert

    FIP200 (RB1CC1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    L324D/R543D/R548D

    Promoter

    Available since

    Dec. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pENTR4 AvrRpm1

    Plasmid

    #209185

    Purpose

    pENTR4 plasmid with CDS of Pseudomonas syringae pv maculicola type III effector AvrRpm1 without stop codon for C-terminal epitope tags

    Depositor

    Lennart Wirthmueller

    Article

    Simranjit et al bioRxiv 2023.09.25.558804

    Insert

    AvrRpm1
    

    Use

    Gateway-compatible entry vector

    Tags

    Expression

    Mutation

    Promoter

    none

    Available since

    Nov. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGL1_38

    Plasmid

    #199045

    Purpose

    Level 1 plasmid, barcoded ORI

    Depositor

    Cultivarium Tools, Nili Ostrov, Charlie Gilbert

    Article

    Gilbert et al bioRxiv 2023.05.19.541510

    Insert

    pIJ101 ORI plus NGS barcode 15
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pGL0_18 [AMA1 long ORI]

    Plasmid

    #198931

    Purpose

    Level 0 part

    Depositor

    Cultivarium Tools, Nili Ostrov, Charlie Gilbert

    Article

    Gilbert et al bioRxiv 2023.05.19.541510

    Insert

    AMA1 long ORI
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLenti-CMV-Myr-Pink Flamindo-p2a-EGFP-bPAC

    Plasmid

    #202717

    Purpose

    Mammalian co-expression of membrane-bound Pink Flamindo and soluble bPAC for cAMP perturbation and imaging

    Depositor

    Adam Cohen

    Article

    Xiang et al bioRxiv 2023

    Inserts

    Myr-Pink Flamindo
    EGFP-bPAC
    

    Use

    Lentiviral

    Tags

    10xHis and myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGL0_38 [Paac(3)-Ia]

    Plasmid

    #198951

    Purpose

    Level 0 part

    Depositor

    Cultivarium Tools, Nili Ostrov, Charlie Gilbert

    Article

    Gilbert et al bioRxiv 2023.05.19.541510

    Insert

    Paac(3)-Ia
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGL0_23 [pSC101ts ORI]

    Plasmid

    #198936

    Purpose

    Level 0 part

    Depositor

    Cultivarium Tools, Nili Ostrov, Charlie Gilbert

    Article

    Gilbert et al bioRxiv 2023.05.19.541510

    Insert

    pSC101ts ORI
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge