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pAG415Gal_Gcn4 ILVtoAS

Plasmid

#232959

Purpose

Galactose iduced expression of Gcn4 ILVtoAS in yeast

Depositor

Aseem Ansari, Tanja Mittag

Publication

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 ILVtoAS

Tags

TEV cleavage site

Expression

Yeast

Mutation

L123S, I128S, V130A, V135A, L137S, I142S

Promoter

Gal1

Available since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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JDW 1394 (pME-ZIC3-P2A-H2A-mCherry)

Plasmid

#232133

Purpose

A middle entry gateway clone containing V5 tagged human ZIC3 and an H2A tagged mCherry, separated by a P2A peptide cleavage sequence.

Depositor

Joshua Wythe

Publication

Cantu Gutierrez et al bioRxiv 2021.11.15.468651

Insert

ZIC3 (ZIC3 Human)

Use

Gateway Cloning

Tags

V5

Available since

Feb. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pENTR4 HopU1

Plasmid

#228395

Purpose

pENTR4 plasmid with CDS of Pseudomonas syringae pv tomato type III effector HopU1 without stop codon for C-terminal epitope tags

Depositor

Lennart Wirthmueller

Publication

Simranjit et al bioRxiv 2023.09.25.558804

Insert

HopU1

Use

Gateway Cloning

Mutation

Alanine inserted after start Methionine due to Nc…

Available since

Feb. 27, 2025

Availability

Academic Institutions and Nonprofits only

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JDW 1051 (pME_V5_GATA2_P2A_H2A_mCherry)

Plasmid

#232128

Purpose

A middle entry gateway clone containing V5 tagged human GATA2 and an H2A tagged mCherry, separated by a P2A peptide cleavage sequence.

Depositor

Joshua Wythe

Publication

Cantu Gutierrez et al bioRxiv 2021.11.15.468651

Insert

GATA2 (GATA2 Human)

Use

Gateway Cloning

Tags

V5

Available since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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JDW 1034 (pME_V5_TCF7_P2A_H2A_mCherry)

Plasmid

#232124

Purpose

A middle entry gateway clone containing V5 tagged human TCF7 and an H2A tagged mCherry, separated by a P2A peptide cleavage sequence.

Depositor

Joshua Wythe

Publication

Cantu Gutierrez et al bioRxiv 2021.11.15.468651

Insert

TCF7 (TCF7 Human)

Use

Gateway Cloning

Tags

V5

Available since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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JDW 1031 (pME_V5_LEF1_P2A_H2A_mCherry)

Plasmid

#232123

Purpose

A middle entry gateway clone containing V5 tagged human LEF1 and an H2A tagged mCherry, separated by a P2A peptide cleavage sequence.

Depositor

Joshua Wythe

Publication

Cantu Gutierrez et al bioRxiv 2021.11.15.468651

Insert

LEF1 (LEF1 Human)

Use

Gateway Cloning

Tags

V5

Available since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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JDW 1035 (pME_V5_TCF7L1_P2A_H2A_mCherry)

Plasmid

#232125

Purpose

A middle entry gateway clone containing V5 tagged human TCFL7 / TCF3 and an H2A tagged mCherry, separated by a P2A peptide cleavage sequence.

Depositor

Joshua Wythe

Publication

Cantu Gutierrez et al bioRxiv 2021.11.15.468651

Insert

TCF7L1 / TCF3 (TCF7L1 Human)

Use

Gateway Cloning

Tags

V5

Available since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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JDW 1052 (pME_V5_MAFB_P2A_H2A_mCherry)

Plasmid

#232129

Purpose

A middle entry gateway clone containing V5 tagged human MAFB and an H2A tagged mCherry, separated by a P2A peptide cleavage sequence.

Depositor

Joshua Wythe

Publication

Cantu Gutierrez et al bioRxiv 2021.11.15.468651

Insert

MAFB (MAFB Human)

Use

Gateway Cloning

Tags

V5

Available since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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JDW 1059 (pME_V5_GATA4_P2A_H2A_mCherry)

Plasmid

#232130

Purpose

A middle entry gateway clone containing V5 tagged human GATA4 and an H2A tagged mCherry, separated by a P2A peptide cleavage sequence.

Depositor

Joshua Wythe

Publication

Cantu Gutierrez et al bioRxiv 2021.11.15.468651

Insert

GATA4 (GATA4 Human)

Use

Gateway Cloning

Tags

V5

Available since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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JDW 1036 (pME_V5_TCF7L2_P2A_H2A_mCherry)

Plasmid

#232126

Purpose

A middle entry gateway clone containing V5 tagged human TCF7L2 / TCF4 and an H2A tagged mCherry, separated by a P2A peptide cleavage sequence.

Depositor

Joshua Wythe

Publication

Cantu Gutierrez et al bioRxiv 2021.11.15.468651

Insert

TCF7L2 / TCF4 (TCF7L2 Human)

Use

Gateway Cloning

Tags

V5

Available since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pGLU_10

Plasmid

#225818

Purpose

Tn libraries Himar variant TET backbone

Depositor

Nili Ostrov

Publication

Gilbert et al bioRxiv 2025.12.22.696024

Type

Empty backbone

Use

Synthetic Biology

Mutation

N/A

Available since

Feb. 11, 2025

Availability

Academic Institutions and Nonprofits only

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OSCA1.2-L438K-peGFP-N1

Plasmid

#232023

Purpose

Expresses OSCA1.2-L438K-peGFP in mammalian cells

Depositor

Huanghe Yang

Publication

Lowry et al bioRxiv [Preprint]. 2024 Sep 3:2024.02.0

Insert

OSCA1.2

Tags

peGFP

Expression

Mammalian

Mutation

L438K

Available since

Feb. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pBEL2838

Plasmid

#231677

Purpose

Expresses cytoplasmic sfGFP in mammalian cells

Depositor

Damian Ekiert

Publication

Jaroenlak et al bioRxiv [Preprint]. 2024 May 30:2024.05.

Insert

sfGFP

Tags

sfGFP

Expression

Mammalian

Available since

Jan. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pGLU_119

Plasmid

#225799

Purpose

RS421 library variant 14434 in pGLDV_18 (p15A)

Depositor

Nili Ostrov

Publication

Gilbert et al bioRxiv 2025.12.22.696024

Insert

14434_[Tn5]

Use

Synthetic Biology

Mutation

N/A

Available since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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OSCA1.2-K435L-peGFP-N1

Plasmid

#228586

Purpose

Expresses OSCA1.2-K435L-eGFP in mammalian cells

Depositor

Huanghe Yang

Publication

Lowry et al bioRxiv [Preprint]. 2024 Sep 3:2024.02.0

Insert

hyperosmolality-gated Ca2+ permeable channel (OSCA) 1.2 (AT4G22120 Mustard Weed)

Expression

Mammalian

Mutation

K435L

Available since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn-DiO-eGFP-2A-mCherry_YDA

Plasmid

#194881

Purpose

Ratiometric YDA sensor for in vivo cre-dependent neuron-specific cholesterol visualization

Depositor

Peter Scheiffele

Publication

Czernecki et al bioRxiv 2022.11.16.516849

Insert

GFP-T2A-mCherry-YDA

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

human Synapsin

Available since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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OSCA1.2-A439K-peGFP-N1

Plasmid

#228589

Purpose

Expresses OSCA1.2-A439K-eGFP in mammalian cells

Depositor

Huanghe Yang

Publication

Lowry et al bioRxiv [Preprint]. 2024 Sep 3:2024.02.0

Insert

hyperosmolality-gated Ca2+ permeable channel (OSCA) 1.2 (AT4G22120 Mustard Weed)

Expression

Mammalian

Mutation

A439K

Available since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pTBL435

Plasmid

#226628

Purpose

pcDNA-dTdT

Depositor

Chang Liu

Publication

Carlson et al bioRxiv [Preprint]. 2024 Jun 11:2024.06.

Insert

catalytically deactivated TdT (DNTT Human)

Use

Synthetic Biology

Expression

Mammalian

Mutation

D343E, D345E

Promoter

CMV

Available since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pGLU_8

Plasmid

#225816

Purpose

Tn libraries Himar variant GEN backbone

Depositor

Nili Ostrov

Publication

Gilbert et al bioRxiv 2025.12.22.696024

Type

Empty backbone

Use

Synthetic Biology

Mutation

N/A

Available since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pGLU_7

Plasmid

#225815

Purpose

Tn libraries Himar variant KAN backbone

Depositor

Nili Ostrov

Publication

Gilbert et al bioRxiv 2025.12.22.696024

Type

Empty backbone

Use

Synthetic Biology

Mutation

N/A

Available since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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