We narrowed to 8,953 results for: plat

1. pCSD_SpCas9MT2-NLS-3xHA-NLS-SaCas9WT-2xNLS

   Plasmid

   #107321

   Purpose

   Expresses SpCas9 (R1333S) fused to SaCas9 in mammalian cells

   Depositor

   Scot Wolfe

   Article

   Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

   Insert

   SpCas9-SaCas9 fusion
   

   Use

   CRISPR

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   SpCas9 (R1333S) is fused to SaCas9

   Promoter

   CMV IE94

   Available Since

   Nov. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. PRR4-bio-His

   Plasmid

   #52075

   Purpose

   Expresses full-length Proline-rich protein 4 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

   Depositor

   Gavin Wright

   Article

   Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

   Insert

   PRR4 (PRR4 Human)
   

   Tags

   His tag, enzymatic biotinylation sequence, and ra…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. TM9-bio-His

   Plasmid

   #51831

   Purpose

   Expresses full-length Transmembrane 9 superfamily member 4 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

   Depositor

   Gavin Wright

   Article

   Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

   Insert

   TM9 (TM9SF4 Human)
   

   Tags

   His tag, enzymatic biotinylation sequence, and ra…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. G6B-bio-His

   Plasmid

   #51644

   Purpose

   Expresses full-length Protein G6b precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

   Depositor

   Gavin Wright

   Article

   Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

   Insert

   G6B (MPIG6B Human)
   

   Tags

   His tag, enzymatic biotinylation sequence, and ra…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pT7-gRNA:Asip

   Plasmid

   #60968

   Purpose

   Expression vector of rat Asip guide RNA

   Depositor

   Tomoji Mashimo

   Article

   Yoshimi et al Nat Commun. 2014 Jun 26;5:4240. doi: 10.

   Insert

   Agouti signaling protein gRNA (Asip Rat)
   

   Use

   CRISPR

   Available Since

   Jan. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

6. pT7-gRNA:kit-1

   Plasmid

   #60969

   Purpose

   Expression vector of rat Kit-1 guide RNA

   Depositor

   Tomoji Mashimo

   Article

   Yoshimi et al Nat Commun. 2014 Jun 26;5:4240. doi: 10.

   Insert

   v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog gRNA (Kit Rat)
   

   Use

   CRISPR

   Available Since

   Jan. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

7. pT7-gRNA:kit-2-1

   Plasmid

   #60970

   Purpose

   Expression vector of rat Kit-2-1 guide RNA

   Depositor

   Tomoji Mashimo

   Article

   Yoshimi et al Nat Commun. 2014 Jun 26;5:4240. doi: 10.

   Insert

   v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog gRNA (Kit Rat)
   

   Use

   CRISPR

   Available Since

   Jan. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

8. pT7-gRNA:kit-2-2

   Plasmid

   #60971

   Purpose

   Expression vector of rat Kit-2-2 guide RNA

   Depositor

   Tomoji Mashimo

   Article

   Yoshimi et al Nat Commun. 2014 Jun 26;5:4240. doi: 10.

   Insert

   v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog gRNA (Kit Rat)
   

   Use

   CRISPR

   Available Since

   Jan. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

9. pC13N-dCas9-BFP-KRAB

   Plasmid

   #127968

   Purpose

   constitutive expression of dCas9-BFP-KRAB from the CLYBL locus (Ward lab)

   Depositor

   Martin Kampmann

   Article

   Tian et al Neuron. 2019 Aug 5. pii: S0896-6273(19)3

   Insert

   CLYBL-CAG-dCas9-NLS-BFP-KRAB
   

   Use

   TALEN

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Aug. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pT7-hMLH1dn for IVT

    Plasmid

    #178114

    Purpose

    Template for in vitro transcription of human MLH1dn

    Depositor

    David Liu

    Article

    Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

    Insert

    human MLH1dn (codon optimized) (MLH1 Human)
    

    Use

    Template for in vitro transcription

    Mutation

    Detailed in manuscript

    Promoter

    T7 (inactivated)

    Available Since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. lenti sgRNA(MS2)_puro optimized backbone

    Plasmid

    #73797

    Purpose

    optimized lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2 and EF1a-puro resistance marker. Contains BsmBI sites for insertion of spacer sequences.

    Depositor

    Feng Zhang

    Article

    Joung et al Nat Protoc. 2017 Apr;12(4):828-863. doi:

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6 and EF1A

    Available Since

    March 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. AAVS1-MAFB-IRF8-CEBPA

    Plasmid

    #190720

    Purpose

    For microglia differentiation from iPSC

    Depositor

    Li Gan

    Article

    Drager et al Nat Neurosci. 2022 Aug 11. pii: 10.1038/

    Insert

    MAFB-IRF8-CEBPA
    

    Expression

    Mammalian

    Available Since

    May 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pENTR221-JUN

    Plasmid

    #79512

    Purpose

    MultiSite Gateway entry clones, second fragment (attL5, attL2) Human JUN CDS

    Depositor

    Peter Jon Nelson

    Article

    Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

    Insert

    JUN (JUN Human)
    

    Use

    Multisite gateway entry clone

    Tags

    HA Tag

    Promoter

    no Promoter

    Available Since

    May 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pENTR221-FOS

    Plasmid

    #79511

    Purpose

    MultiSite Gateway entry clones, second fragment (attL5, attL2) Human FOS CDS

    Depositor

    Peter Jon Nelson

    Article

    Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

    Insert

    FOS (FOS Human)
    

    Use

    Multisite gateway entry clone

    Promoter

    no Promoter

    Available Since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. GSN-bio-His

    Plasmid

    #52067

    Purpose

    Expresses full-length Gelsolin precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    GSN (GSN Human)
    

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLVX-CMV-Galpha-oA ONE-GO

    Plasmid

    #189732

    Purpose

    GPCR/G protein BRET biosensor

    Depositor

    Mikel Garcia-Marcos

    Article

    Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

    Inserts

    Galpha-oA ONE-GO
    GNAO (Gnao1 Rat)
    

    Use

    Lentiviral

    Mutation

    I250 (silent mutation in YFP)

    Available Since

    Jan. 19, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. pGL4_NFAT-RE-CMVmin-BC0524-luc2

    Plasmid

    #227139

    Purpose

    Barcoded assay, Ca2+ sensor; barcode BC0524

    Depositor

    Michael Wehr

    Article

    Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

    Insert

    6x clustered NFAT element linked to CMV minimal promoter driving barcode 0524 and a luciferase reporter gene
    

    Expression

    Mammalian

    Available Since

    Nov. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. CD47-bio-His

    Plasmid

    #53150

    Purpose

    Expresses full-length CD47 ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    CD47 (CD47 Human)
    

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCMV-EGFP-eDHFR(69K6)-MCS

    Plasmid

    #172100

    Purpose

    Mammalian expression of a protein of interest fused to the C-terminus of EGFP-eDHFR(69K6)

    Depositor

    Shinya Tsukiji

    Article

    Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

    Insert

    EGFP-eDHFR(69K6)-20aa
    

    Expression

    Mammalian

    Mutation

    eDHFR(69K6): Hexalysine (K6) sequence is inserted…

    Promoter

    CMV

    Available Since

    July 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. MAVS-WT

    Plasmid

    #52135

    Purpose

    Retroviral vector for expression of human MAVS in mammalian cells

    Depositor

    Jonathan Kagan

    Article

    Dixit et al Cell. 2010 May 14;141(4):668-81. Epub 20

    Insert

    MAVS (MAVS Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    Aug. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge