165,839 results

1. pHAGE-NLS-dPguCas13b-3xmRuby3-NLS

   Plasmid

   #132406

   Purpose

   overexpression in human cells

   Depositor

   Ling-Ling Chen

   Article

   Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

   Insert

   dPguCas13b
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   H151A; H1121A

   Promoter

   Available Since

   Aug. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3-Flag::UbvG08 I44A, deltaGG

   Plasmid

   #74939

   Purpose

   Ubiquitin variant that binds to and occludes the ligand binding site of the 53BP1 Tudor domain (i53). Blocks 53BP1 from accumulating at sites of DNA damage. Potent selective inhibitor of 53BP1

   Depositor

   Daniel Durocher

   Article

   Canny et al Nat Biotechnol. 2018 Jan;36(1):95-102. d

   Insert

   Ubiquitin
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   UbvG08 with I44A mutation and no terminal Glycines

   Promoter

   CMV

   Available Since

   June 30, 2016

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pSBbi 41BBL-CD86 mIL-15

   Plasmid

   #183245

   Purpose

   Expression of human 41BBL, CD86, and membrane bound human IL-15 (IL-15-IL-15Ra) in a Sleeping Beauty transposon vector for use in artificial antigen presenting cells

   Depositor

   Alexander McLellan

   Article

   Dobson et al Immunol Cell Biol. 2023 Aug 16. doi: 10.

   Insert

   41BBL, CD86, mbIL-15 (IL-15-IL-15Ra)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV_BiPVe3_ChR2_mCherry

   Plasmid

   #213941

   Purpose

   AAV construct expressing mCherry-tagged channelrhodopsin-2 driven by PV+ basket cell-targeting enhancer. Alias: AAV_WDP0003_ChR2_mCherry

   Depositor

   Gordon Fishell

   Article

   Furlanis et al Neuron. 2025 May 21;113(10):1525-1547.e1

   Has Service

   AAV PHP.eB and AAV1

   Insert

   Channelrhodopsin 2-mCherry
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   July 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEGFP-N1-human lyn–GFP

   Plasmid

   #35958

   Purpose

   Expresses human lyn protein codon optimized for zebrafish expression.

   Depositor

   Anna Huttenlocher

   Article

   Yoo et al Nature. 2011 Nov 20;480(7375):109-12. do

   Insert

   LYN (LYN Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   wt

   Promoter

   cmv

   Available Since

   April 27, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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6. Pseudojanin (PJ)

   Plasmid

   #37999

   Depositor

   Robin Irvine

   Article

   Hammond et al Science. 2012 Jun 21.

   Inserts

   FKBP1A (FKBP1A Human)
   SAC1
   INPP5E (INPP5E Human)
   

   Use

   Tags

   mRFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 13, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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7. FUW-TetON-GFP

   Plasmid

   #115495

   Purpose

   Lentiviral plasmid for tet-inducible expression of GFP (generated from plasmid #20321)

   Depositor

   Catherine Verfaillie

   Article

   Garcia-Leon et al Stem Cell Reports. 2018 Feb 13;10(2):655

   Insert

   eGFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Nov. 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3-HA-14-3-3 gamma

   Plasmid

   #13274

   Depositor

   Michael Yaffe

   Insert

   14-3-3 gamma (Ywhag Rat)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 7, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJMP3

   Plasmid

   #79875

   Purpose

   Bacillus subtilis sgRNA expression vector; integrates into thrC

   Depositor

   Jason Peters, Stanley Qi, Carol Gross

   Article

   Peters et al Cell. 2016 Jun 2;165(6):1493-506. doi: 1

   Insert

   sgRNA RR1
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   veg

   Available Since

   Sept. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. v3em-Cterm-PE6c-dualU6-Rosa26-twinPE-attB

    Plasmid

    #207861

    Purpose

    AAV genome encoding C-terminal PE6c and U6 expression cassettes for Rosa26 twinPE pegRNA pairs

    Depositor

    David Liu

    Article

    Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

    Insert

    NpuC-Cterm-PE6c-dualU6-Rosa26-twinPE-attB
    

    Use

    AAV

    Tags

    Expression

    Mutation

    See Manuscript

    Promoter

    Cbh

    Available Since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGP-AAV-syn-jGCaMP8f-WPRE (AAV9)

    Viral Prep

    #162376-AAV9

    Purpose

    Ready-to-use AAV9 particles produced from pGP-AAV-syn-jGCaMP8f-WPRE (#162376). In addition to the viral particles, you will also receive purified pGP-AAV-syn-jGCaMP8f-WPRE plasmid DNA. Syn-driven expression of ultrafast calcium sensor GCaMP8f. These AAV preparations are suitable purity for injection into animals.

    Depositor

    GENIE Project

    Article

    Zhang et al Janelia Research Campus, Online Resource

    Promoter

    Syn

    Tags

    None

    Available Since

    May 19, 2021

    Availability

    Academic Institutions and Nonprofits only

12. PiggyBac PlayBack

    Plasmid

    #203312

    Purpose

    To all for PiggyBac gene insertion into Genome

    Depositor

    Aleksandar Necakov

    Article

    Foran et al Biotechniques. 2023 Oct;75(4):168-178. d

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. LV-YFP

    Plasmid

    #26000

    Depositor

    Elaine Fuchs

    Article

    Beronja et al Nat Med. 2010 Jul . 16(7):821-7.

    Insert

    H2B-YFP (H2BC21 Human, A. victoria)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 10, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pBAD/His-miRFP670nano3

    Plasmid

    #184663

    Purpose

    Monomeric near-infrared fluorescent protein miRFP670nano3 in bacterial plasmid

    Depositor

    Vladislav Verkhusha

    Article

    Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

    Insert

    miRFP670nano3
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    araC

    Available Since

    June 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. TFORF2531

    Plasmid

    #142954

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    SOX9 (SOX9 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    June 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDEST-ORF-V2

    Plasmid

    #73638

    Purpose

    Gateway™ destination vector encoding C-terminal fusion with Venus fluorescent protein fragment 2 (V2).

    Depositor

    Darren Saunders

    Article

    Croucher et al Sci Signal. 2016 Jul 12;9(436):ra69. doi

    Type

    Empty backbone

    Use

    Tags

    Venus fragment 2 (V2)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. mTurquoise-Rab5a-7

    Plasmid

    #55578

    Purpose

    Localization: Ras GTPase / Endosomes, Excitation: 434, Emission: 474

    Depositor

    Michael Davidson

    Insert

    Rab5a
    

    Use

    Tags

    mTurquoise

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 22, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

18. Galphai3-mTurquoise2

    Plasmid

    #69622

    Purpose

    The heterotrimeric G-protein subunit, Gαi isoform 3, tagged with mTurquoise2

    Depositor

    Dorus Gadella

    Article

    van Unen et al PLoS One. 2016 Jan 22;11(1):e0146789. do

    Insert

    Galphai3-mTurquoise2
    

    Use

    Tags

    mTurquoise2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAW016-2

    Plasmid

    #85613

    Purpose

    Cas9-tracrRNA integration vector for Bacillus subtilis

    Depositor

    Perry Chou

    Article

    Westbrook et al Appl Environ Microbiol. 2016 Jun 3. pii:

    Insert

    cas9-tracrRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSC101-pT7-T3RNAP

    Plasmid

    #140872

    Purpose

    T7 promoter driven synthesis of T3 RNA polymerase

    Depositor

    Neal Devaraj

    Article

    Niederholtmeyer et al Nat Commun. 2018 Nov 28;9(1):5027. doi:

    Insert

    T3 RNA polymerase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available Since

    May 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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