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A SARS-CoV-2 protein interaction map reveals targets for drug repurposing.
Gordon DE, Jang GM, Bouhaddou M, Xu J, Obernier K, White KM, O'Meara MJ, Rezelj VV, Guo JZ, Swaney DL, Tummino TA, Huttenhain R, Kaake RM, Richards AL, Tutuncuoglu B, Foussard H, Batra J, Haas K, Modak M, Kim M, Haas P, Polacco BJ, Braberg H, Fabius JM, Eckhardt M, Soucheray M, Bennett MJ, Cakir M, McGregor MJ, Li Q, Meyer B, Roesch F, Vallet T, Mac Kain A, Miorin L, Moreno E, Naing ZZC, Zhou Y, Peng S, Shi Y, Zhang Z, Shen W, Kirby IT, Melnyk JE, Chorba JS, Lou K, Dai SA, Barrio-Hernandez I, Memon D, Hernandez-Armenta C, Lyu J, Mathy CJP, Perica T, Pilla KB, Ganesan SJ, Saltzberg DJ, Rakesh R, Liu X, Rosenthal SB, Calviello L, Venkataramanan S, Liboy-Lugo J, Lin Y, Huang XP, Liu Y, Wankowicz SA, Bohn M, Safari M, Ugur FS, Koh C, Savar NS, Tran QD, Shengjuler D, Fletcher SJ, O'Neal MC, Cai Y, Chang JCJ, Broadhurst DJ, Klippsten S, Sharp PP, Wenzell NA, Kuzuoglu-Ozturk D, Wang HY, Trenker R, Young JM, Cavero DA, Hiatt J, Roth TL, Rathore U, Subramanian A, Noack J, Hubert M, Stroud RM, Frankel AD, Rosenberg OS, Verba KA, Agard DA, Ott M, Emerman M, Jura N, von Zastrow M, Verdin E, Ashworth A, Schwartz O, d'Enfert C, Mukherjee S, Jacobson M, Malik HS, Fujimori DG, Ideker T, Craik CS, Floor SN, Fraser JS, Gross JD, Sali A, Roth BL, Ruggero D, Taunton J, Kortemme T, Beltrao P, Vignuzzi M, Garcia-Sastre A, Shokat KM, Shoichet BK, Krogan NJ
Nature. 2020 Jul;583(7816):459-468. doi: 10.1038/s41586-020-2286-9. Epub 2020 Apr 30.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
141367pLVX-EF1alpha-SARS-CoV-2-nsp1-2xStrep-IRES-PuroLentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus
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141368pLVX-EF1alpha-SARS-CoV-2-nsp2-2xStrep-IRES-PuroLentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus
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141369pLVX-EF1alpha-SARS-CoV-2-nsp4-2xStrep-IRES-PuroLentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus
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141370pLVX-EF1alpha-SARS-CoV-2-nsp5-2xStrep-IRES-PuroLentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus
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141371pLVX-EF1alpha-SARS-CoV-2-nsp5-C145A-2xStrep-IRES-PuroLentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus
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141372pLVX-EF1alpha-SARS-CoV-2-nsp6-2xStrep-IRES-PuroLentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus
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141373pLVX-EF1alpha-SARS-CoV-2-nsp7-2xStrep-IRES-PuroLentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus
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141374pLVX-EF1alpha-SARS-CoV-2-nsp8-2xStrep-IRES-PuroLentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus
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141375pLVX-EF1alpha-SARS-CoV-2-nsp9-2xStrep-IRES-PuroLentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus
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141376pLVX-EF1alpha-SARS-CoV-2-nsp10-2xStrep-IRES-PuroLentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus
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141377pLVX-EF1alpha-SARS-CoV-2-nsp11-2xStrep-IRES-PuroLentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus
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141378pLVX-EF1alpha-SARS-CoV-2-nsp12-2xStrep-IRES-PuroLentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus
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141379pLVX-EF1alpha-SARS-CoV-2-nsp13-2xStrep-IRES-PuroLentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus
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141380pLXV-EF1alpha-2xStrep-SARS-CoV-2-nsp14-IRES-PuroLentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus
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141381pLVX-EF1alpha-SARS-CoV-2-nsp15-2xStrep-IRES-PuroLentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus
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141383pLVX-EF1alpha-SARS-CoV-2-orf3a-2xStrep-IRES-PuroLentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus
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141384pLXV-EF1alpha-2xStrep-SARS-CoV-2-orf3b-IRES-PuroLentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus
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141385pLVX-EF1alpha-SARS-CoV-2-E-2xStrep-IRES-PuroLentiviral expression of SARS-CoV-2 envelope (E) protein; transient expression and generate lentivirus
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141386pLVX-EF1alpha-SARS-CoV-2-M-2xStrep-IRES-PuroLentiviral expression of SARS-CoV-2 membrane (M) protein; transient expression and generate lentivirus
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141387pLVX-EF1alpha-SARS-CoV-2-orf6-2xStrep-IRES-PuroLentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus
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141388pLVX-EF1alpha-SARS-CoV-2-orf7a-2xStrep-IRES-PuroLentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus
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141389pLXV-EF1alpha-2xStrep-SARS-CoV-2-orf7b-IRES-PuroLentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus
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141390pLVX-EF1alpha-SARS-CoV-2-orf8-2xStrep-IRES-PuroLentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus
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141391pLVX-EF1alpha-SARS-CoV-2-N-2xStrep-IRES-PuroLentiviral expression of SARS-CoV-2 nucleocapsid (N) protein; transient expression and generate lentivirus
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141392pLVX-EF1alpha-SARS-CoV-2-orf9b-2xStrep-IRES-PuroLentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus
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141393pLXV-EF1alpha-2xStrep-SARS-CoV-2-orf9c-IRES-PuroLentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus
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141394pLVX-EF1alpha-SARS-CoV-2-orf10-2xStrep-IRES-PuroLentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus
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141395pLVX-EF1alpha-eGFP-2xStrep-IRES-PuroLentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus
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Recombinant Antibodies from Article