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A SARS-CoV-2 protein interaction map reveals targets for drug repurposing.
Gordon DE, Jang GM, Bouhaddou M, Xu J, Obernier K, White KM, O'Meara MJ, Rezelj VV, Guo JZ, Swaney DL, Tummino TA, Huttenhain R, Kaake RM, Richards AL, Tutuncuoglu B, Foussard H, Batra J, Haas K, Modak M, Kim M, Haas P, Polacco BJ, Braberg H, Fabius JM, Eckhardt M, Soucheray M, Bennett MJ, Cakir M, McGregor MJ, Li Q, Meyer B, Roesch F, Vallet T, Mac Kain A, Miorin L, Moreno E, Naing ZZC, Zhou Y, Peng S, Shi Y, Zhang Z, Shen W, Kirby IT, Melnyk JE, Chorba JS, Lou K, Dai SA, Barrio-Hernandez I, Memon D, Hernandez-Armenta C, Lyu J, Mathy CJP, Perica T, Pilla KB, Ganesan SJ, Saltzberg DJ, Rakesh R, Liu X, Rosenthal SB, Calviello L, Venkataramanan S, Liboy-Lugo J, Lin Y, Huang XP, Liu Y, Wankowicz SA, Bohn M, Safari M, Ugur FS, Koh C, Savar NS, Tran QD, Shengjuler D, Fletcher SJ, O'Neal MC, Cai Y, Chang JCJ, Broadhurst DJ, Klippsten S, Sharp PP, Wenzell NA, Kuzuoglu-Ozturk D, Wang HY, Trenker R, Young JM, Cavero DA, Hiatt J, Roth TL, Rathore U, Subramanian A, Noack J, Hubert M, Stroud RM, Frankel AD, Rosenberg OS, Verba KA, Agard DA, Ott M, Emerman M, Jura N, von Zastrow M, Verdin E, Ashworth A, Schwartz O, d'Enfert C, Mukherjee S, Jacobson M, Malik HS, Fujimori DG, Ideker T, Craik CS, Floor SN, Fraser JS, Gross JD, Sali A, Roth BL, Ruggero D, Taunton J, Kortemme T, Beltrao P, Vignuzzi M, Garcia-Sastre A, Shokat KM, Shoichet BK, Krogan NJ
Nature. 2020 Jul;583(7816):459-468. doi: 10.1038/s41586-020-2286-9. Epub 2020 Apr 30. PubMed Article

Plasmids from Article

ID Plasmid Purpose
141367pLVX-EF1alpha-SARS-CoV-2-nsp1-2xStrep-IRES-PuroLentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus
141368pLVX-EF1alpha-SARS-CoV-2-nsp2-2xStrep-IRES-PuroLentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus
141369pLVX-EF1alpha-SARS-CoV-2-nsp4-2xStrep-IRES-PuroLentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus
141370pLVX-EF1alpha-SARS-CoV-2-nsp5-2xStrep-IRES-PuroLentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus
141371pLVX-EF1alpha-SARS-CoV-2-nsp5-C145A-2xStrep-IRES-PuroLentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus
141373pLVX-EF1alpha-SARS-CoV-2-nsp7-2xStrep-IRES-PuroLentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus
141374pLVX-EF1alpha-SARS-CoV-2-nsp8-2xStrep-IRES-PuroLentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus
141375pLVX-EF1alpha-SARS-CoV-2-nsp9-2xStrep-IRES-PuroLentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus
141376pLVX-EF1alpha-SARS-CoV-2-nsp10-2xStrep-IRES-PuroLentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus
141377pLVX-EF1alpha-SARS-CoV-2-nsp11-2xStrep-IRES-PuroLentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus
141378pLVX-EF1alpha-SARS-CoV-2-nsp12-2xStrep-IRES-PuroLentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus
141379pLVX-EF1alpha-SARS-CoV-2-nsp13-2xStrep-IRES-PuroLentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus
141380pLXV-EF1alpha-2xStrep-SARS-CoV-2-nsp14-IRES-PuroLentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus
141381pLVX-EF1alpha-SARS-CoV-2-nsp15-2xStrep-IRES-PuroLentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus
141383pLVX-EF1alpha-SARS-CoV-2-orf3a-2xStrep-IRES-PuroLentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus
141384pLXV-EF1alpha-2xStrep-SARS-CoV-2-orf3b-IRES-PuroLentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus
141385pLVX-EF1alpha-SARS-CoV-2-E-2xStrep-IRES-PuroLentiviral expression of SARS-CoV-2 envelope (E) protein; transient expression and generate lentivirus
141386pLVX-EF1alpha-SARS-CoV-2-M-2xStrep-IRES-PuroLentiviral expression of SARS-CoV-2 membrane (M) protein; transient expression and generate lentivirus
141387pLVX-EF1alpha-SARS-CoV-2-orf6-2xStrep-IRES-PuroLentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus
141388pLVX-EF1alpha-SARS-CoV-2-orf7a-2xStrep-IRES-PuroLentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus
141389pLXV-EF1alpha-2xStrep-SARS-CoV-2-orf7b-IRES-PuroLentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus
141390pLVX-EF1alpha-SARS-CoV-2-orf8-2xStrep-IRES-PuroLentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus
141391pLVX-EF1alpha-SARS-CoV-2-N-2xStrep-IRES-PuroLentiviral expression of SARS-CoV-2 nucleocapsid (N) protein; transient expression and generate lentivirus
141392pLVX-EF1alpha-SARS-CoV-2-orf9b-2xStrep-IRES-PuroLentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus
141393pLXV-EF1alpha-2xStrep-SARS-CoV-2-orf9c-IRES-PuroLentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus
141394pLVX-EF1alpha-SARS-CoV-2-orf10-2xStrep-IRES-PuroLentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus
141395pLVX-EF1alpha-eGFP-2xStrep-IRES-PuroLentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

Antibodies from Article