|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||141392||Standard format: Plasmid sent in bacteria as agar stab||1||$75 *|
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Backbone manufacturerTakara Bio
- Backbone size w/o insert (bp) 8825
Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
Growth Strain(s)NEB Stable
Mutationhuman codon optimized
- Promoter EF1alpha
/ Fusion Protein
- 2xStrep (C terminal on insert)
- Cloning method Unknown
Terms and Licenses
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pLVX-EF1alpha-SARS-CoV-2-orf9b-2xStrep-IRES-Puro was a gift from Nevan Krogan (Addgene plasmid # 141392 ; http://n2t.net/addgene:141392 ; RRID:Addgene_141392)
For your References section:A SARS-CoV-2-Human Protein-Protein Interaction Map Reveals Drug Targets and Potential Drug Repurposing. Gordon DE, et al.. BioRxiv March 2020 10.1101/2020.03.22.002386