165,839 results

1. pACYCDuet-1:ChrA

   Plasmid

   #225079

   Purpose

   Expresses precursor peptide ChrA in E.coli

   Depositor

   Wilfred van der Donk

   Article

   Ayikpoe et al ACS Cent Sci. 2023 Apr 24;9(5):1008-1018

   Insert

   ChrA
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   T7

   Available Since

   Jan. 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pH2B-Electra2

   Plasmid

   #179479

   Purpose

   Nuclear labeling with blue fluorescent protein Electra2 fused to H2B

   Depositor

   Kiryl Piatkevich

   Article

   Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

   Insert

   H2B-Electra2
   

   Use

   Tags

   Electra2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Aug. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAW014-2

   Plasmid

   #85612

   Purpose

   Multi-gRNA delivery vector containing ugtP-gRNA.P395T for Bacillus subtilis

   Depositor

   Perry Chou

   Article

   Westbrook et al Appl Environ Microbiol. 2016 Jun 3. pii:

   Insert

   ugtP-gRNA.P395T
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Jan. 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pPICZalphaB-SapL3

   Plasmid

   #78171

   Purpose

   Expression of Saporin L3 in pichia

   Depositor

   Vern Schramm

   Article

   Yuan et al Biochemistry. 2015 Jul 28;54(29):4565-74

   Insert

   Saporin L3
   

   Use

   Tags

   alpha factor

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   July 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEIAV-SIN6.1 CGFPW

   Plasmid

   #44171

   Depositor

   John Olsen

   Article

   McKay et al Gene Ther. 2006 Apr;13(8):715-24.

   Insert

   enhanced green fluorescent protein
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   chromophore mutations

   Promoter

   CMV

   Available Since

   May 17, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSLQ14901

   Plasmid

   #239276

   Purpose

   Expresses gG1 for pertubing endogenous human GAPDH mRNA via CRISPR-TO

   Depositor

   Stanley Qi

   Article

   Han et al Nature. 2025 Jul;643(8070):241-251. doi:

   Insert

   PspCas13b-hGAPDH-gRNA1 (GAPDH Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available Since

   Sept. 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pK27Sumo_His-SUMO-PLpro (nsp3) (SARS-CoV-2)

   Plasmid

   #169193

   Purpose

   To express SARS-CoV-2 Papain-like protease in E. coli

   Depositor

   John Diffley

   Article

   Lim et al Biochem J. 2021 Jul 16;478(13):2517-2531

   Insert

   14His-SUMO-nsp3_E746-K1060 (pp1ab_E1564-K1878) (ORF1ab SARS-CoV-2, Synthetic)
   

   Use

   Tags

   14His-SUMO

   Expression

   Bacterial

   Mutation

   Codon optimised for E. coli

   Promoter

   T5

   Available Since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV_BiPVe4_Gq_dTomato

   Plasmid

   #213938

   Purpose

   AAV construct expressing HA-hM3Dq-dTomato driven by chandelier cell-targeting enhancer. Alias: pAAV_WDC0004_Gq_dTomato

   Depositor

   Gordon Fishell

   Article

   Furlanis et al Neuron. 2025 May 21;113(10):1525-1547.e1

   Insert

   HA-GqDREADD-dTomato
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   July 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pACλCI-β-flap (831-1057)

   Plasmid

   #53733

   Purpose

   Expression of λCI fused via three alanines to E. coli RNAP beta subunit, aa 831-1057 (β-flap). Can be cut with Not1 and BamHI to replace β-flap with a moiety of interest.

   Depositor

   Ann Hochschild

   Article

   Dove et al Nature. 1997 Apr 10;386(6625):627-30.

   Insert

   λCI-β-flap fusion
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   lac UV5

   Available Since

   June 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBRα-β flap (831-1057)

    Plasmid

    #53734

    Purpose

    Expression of alpha NTD fused via three alanines to E. coli RNAP subunit beta, aa 831-1057 (β-flap). Can be cut with Not1 and BamHI to replace β-flap with a moiety of interest.

    Depositor

    Ann Hochschild

    Article

    Dove et al Nature. 1997 Apr 10;386(6625):627-30.

    Insert

    alpha NTD-Beta flap fusion
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    lpp/lacUV5 (tandem promoter)

    Available Since

    June 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET41b+_Npl4

    Plasmid

    #117108

    Purpose

    Production of recombinant Npl4 in E.coli

    Depositor

    Hemmo Meyer

    Article

    Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

    Insert

    Npl4 (NPLOC4 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDRF1-GW ymNeongreen-T2A-mCherry

    Plasmid

    #125700

    Purpose

    Produces equimolar expression of yeast codon optimized mNeongreen and mCherry

    Depositor

    Bas Teusink

    Article

    Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

    Insert

    ymNeongreen-T2A-mCherry
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    June 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. GFP-ERK2

    Plasmid

    #37145

    Depositor

    Rony Seger

    Article

    Rubinfeld et al J Biol Chem. 1999 Oct 22;274(43):30349-5

    Insert

    ERK2 (MAPK1 Rat)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    aa1-6 deleted as a result of cloning strategy

    Promoter

    CMV

    Available Since

    Nov. 14, 2012

    Availability

    Academic Institutions and Nonprofits only

14. TFORF1335

    Plasmid

    #142786

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    POU3F2 (POU3F2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    June 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEBTet-SNAP-Cep152

    Plasmid

    #136825

    Purpose

    Mammalian expression of the centrosomal protein Cep152 N-terminally fused to SNAP-tag

    Depositor

    Kai Johnsson

    Article

    Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

    Insert

    SNAP-Cep152 (CEP152 Human, Synthetic)
    

    Use

    Tags

    FLAG-tag / His-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-TetO2

    Available Since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pNCS mTangerine

    Plasmid

    #91763

    Purpose

    Bacterial expression of a fluorescent protein, monomeric Tangerine (mTangerine)

    Depositor

    Erik Rodriguez, Roger Tsien

    Article

    Shaner et al Nat Biotechnol. 2004 Dec;22(12):1567-72.

    Insert

    monomeric Tangerine
    

    Use

    Tags

    no tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    June 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. CSII-prEF1a-mCherry-3xNLS

    Plasmid

    #125262

    Purpose

    Fluorescent reporter for cell size

    Depositor

    Jan Skotheim

    Article

    Berenson et al Mol Biol Cell. 2019 Nov 15;30(24):2985-2

    Insert

    mCherry-3xNLS
    

    Use

    Lentiviral

    Tags

    3xNLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    May 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pENTR TERRA

    Plasmid

    #111383

    Purpose

    TERRA with approximately 130 telomere hexanucleotide repeats of TTAGGG and TTGGGG variant sequences

    Depositor

    Klaus Holzmann

    Article

    Kreilmeier et al Genes (Basel). 2016 Aug 16;7(8). pii: ge

    Insert

    TERRA
    

    Use

    Gateway entry cloning

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. MSCV-BCR-ABL1-p190-IRES-EGFP

    Plasmid

    #219964

    Purpose

    Express human BCR-ABL1-P190 in mammalian cells

    Depositor

    Mohamed Fareh

    Insert

    BCR-ABL1-P190 (ABL1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. 24xMoonTag-kif18b-24xPP7 (MoonTag translation reporter)

    Plasmid

    #128604

    Purpose

    Expresses 24xgp41 peptide array (MoonTag peptide array), followed by kif18b gene and 24 repeats of PP7 hairpins in 3’ UTR.

    Depositor

    Marvin Tanenbaum

    Article

    Boersma et al Cell. 2019 Jun 5. pii: S0092-8674(19)304

    Inserts

    24xMoonTag
    kinesin family member 18b (KIF18B Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Pcmv

    Available Since

    Aug. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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