168,037 results

1. pPBT-peRNA_GG-Puro

   Plasmid

   #173220

   Purpose

   piggyBac transposon containing hU6 promotor and pegRNA cloning site

   Depositor

   Jacob Giehm Mikkelsen

   Article

   Wolff et al Front Genome Ed. 2021 Nov 12;3:786893. d

   Inserts

   pegRNA cloning cassette
   PuroR
   

   Use

   Piggybac

   Expression

   Mammalian

   Promoter

   EF1-a and hU6

   Available Since

   May 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHH0103_YWHAZ_14-3-3

   Plasmid

   #109808

   Purpose

   Protein expression and purification of YWHAZ_14-3-3

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

   Insert

   YWHAZ_14-3-3 (YWHAZ Human)
   

   Expression

   Bacterial

   Available Since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pENTR-L1-ESYN-L4

   Plasmid

   #32580

   Depositor

   Matthew Nolan

   Article

   White et al Front Mol Neurosci. 2011;4:8. Epub 2011

   Insert

   Enhanced Synapsin Promoter
   

   Use

   Gateway cloning vector

   Available Since

   April 11, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTXB1-nbMmKappa-Tn5

   Plasmid

   #184286

   Purpose

   Anti-mouse kappa chain specific nanobody-Tn5 for NTT-seq (multiplexed CUT&Tag)

   Depositor

   New York Genome Center, Ivan Raimondi

   Article

   Stuart et al Nat Biotechnol. 2022 Dec 19. doi: 10.103

   Insert

   nbMmKappa-Tn5
   

   Available Since

   May 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSC5

   Plasmid

   #104779

   Purpose

   pSC218GG-Glycine max Ubiquitin:Gateway expression cassette binary vector

   Depositor

   Robert Stupar

   Article

   Curtin et al Plant Biotechnol J. 2017 Oct 31. doi: 10

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Plant

   Available Since

   Jan. 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. 5KKP (PUS7)

   Plasmid

   #101878

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   PUS7 (PUS7 Human)
   

   Tags

   6xHis, TEV

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   April 30, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pJ2018

   Plasmid

   #140665

   Purpose

   dCas9, LuxR & Csy4

   Depositor

   Yolanda Schaerli

   Article

   Santos-Moreno et al Nat Commun. 2020 Jun 2;11(1):2746. doi:

   Insert

   dCas9, LuxR & Csy4
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   July 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3-Lyso-AktAR2

   Plasmid

   #64933

   Purpose

   Lysosome-targeted FRET biosensor for monitoring Akt activity; targeted to cytosolic face of lysosomal membrane.

   Depositor

   Jin Zhang

   Article

   Zhou et al Cell Rep. 2015 Mar 11. pii: S2211-1247(1

   Insert

   LAMP1-AktAR2 (LAMP1 Synthetic, Budding Yeast, Human)
   

   Tags

   Cerulean3, Full-length LAMP1, and cpVenus[E172]

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. RP125 pUAST EGFP:Orco

   Plasmid

   #53441

   Purpose

   N-terminal fusion of EGFP to Drosophila melanogaster Orco

   Depositor

   Leslie Vosshall

   Article

   Benton et al PLoS Biol. 2006 Feb;4(2):e20. Epub 2006

   Insert

   EGFP:Orco (Orco Fly)
   

   Tags

   EGFP

   Expression

   Insect

   Available Since

   July 15, 2014

   Availability

   Academic Institutions and Nonprofits only

10. CL20_mEGFP_COL3A1_CLU

    Plasmid

    #205794

    Purpose

    Express mEGFP-tagged fusion protein, COL3A1_CLU from patient-derived sequence

    Depositor

    Richard Kriwacki

    Article

    Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

    Insert

    COL3A1_CLU (COL3A1, CLU Human)
    

    Tags

    mEGFP

    Expression

    Mammalian

    Available Since

    Nov. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. YSBE 516 : pNEO_4xFLAG

    Plasmid

    #92082

    Purpose

    Plasmid for tagging proteins in Cryptococcus neoformans; 4xFLAG tag, Neo selection

    Depositor

    Yong-Sun Bahn

    Article

    So et al J Microbiol Biotechnol. 2017 Feb 28;27(2

    Insert

    4xFlag
    

    Use

    tagging proteins in cryptococcus neoforman

    Available Since

    Oct. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. mCherry-MAP4-C-10

    Plasmid

    #55075

    Purpose

    Localization: Microtubules, Excitation: 587, Emission: 610

    Depositor

    Michael Davidson

    Insert

    MAP4 (Map4 Mouse)
    

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    aa 640-1045 of MAP4

    Promoter

    CMV

    Available Since

    March 2, 2015

    Availability

    Academic Institutions and Nonprofits only

13. V5-LOV-Turbo-NES

    Plasmid

    #199545

    Purpose

    expresses LOV-Turbo in the mammalian cytosol, lentiviral vector

    Depositor

    Alice Ting

    Article

    Lee et al Nat Methods. 2023 May 15. doi: 10.1038/s

    Insert

    LOV-Turbo
    

    Use

    Lentiviral

    Tags

    NES and V5

    Expression

    Mammalian

    Promoter

    TRE3G

    Available Since

    May 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-Ef1a DIO-ChRmine-oScarlet-WPRE

    Plasmid

    #183524

    Purpose

    Optogenetics

    Depositor

    Karl Deisseroth

    Article

    Kishi et al Cell. 2022 Feb 17;185(4):672-689.e23. do

    Insert

    ChRmine-oScarlet
    

    Use

    AAV

    Promoter

    Ef1a

    Available Since

    May 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. Myr.PKCzeta.FLAG

    Plasmid

    #10802

    Depositor

    Alex Toker

    Article

    Chou et al Curr Biol. 1998 Sep 24. 8(19):1069-77.

    Insert

    PKC zeta (Prkcz Rat)
    

    Tags

    FLAG and Myr

    Expression

    Mammalian

    Available Since

    July 26, 2006

    Availability

    Academic Institutions and Nonprofits only

16. GABBR1-DuET

    Plasmid

    #213247

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    GABBR1 (GABBR1 Human)
    

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Promoter

    CMV, T7

    Available Since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-DIO-CaMKII-TVA-P2A-N2cG

    Plasmid

    #172362

    Purpose

    Targeting envA-pseodotyped RVdG-CVS-N2c vectors for specific retrograde labeling from cre-expressing excitatory cortical neurons

    Depositor

    Yoav Ben-Simon, Peter Jonas

    Article

    Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

    Insert

    TVA + B19-N2cG
    

    Use

    AAV and Cre/Lox

    Expression

    Mammalian

    Promoter

    CaMKIIa

    Available Since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. PB-Ef1a-SunTag-RPS7-PP7-WPRE

    Plasmid

    #198338

    Purpose

    PB plasmid expressing RPS7 mRNA tagged with SunTag repeats and PP7 stem loops

    Depositor

    Michael Ward

    Article

    De Pace et al Nat Neurosci. 2024 Apr 10. doi: 10.1038/

    Insert

    GCN4x24-RPS7-3'UTR-PP7 stem loops x24
    

    Use

    Piggybac

    Tags

    PP7 stem loops and SunTag

    Expression

    Mammalian

    Promoter

    Ef1a

    Available Since

    March 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAPM-D4 miR30-MPHOSPH8 ts2

    Plasmid

    #115875

    Purpose

    MPHOSPH8 knockdown

    Depositor

    Jeremy Luban

    Article

    Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

    Insert

    MPHOSPH8 shRNA (MPHOSPH8 Human)
    

    Use

    Lentiviral

    Promoter

    SFFV

    Available Since

    Jan. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pTH727-CEN-RLuc/staCFLuc

    Plasmid

    #38211

    Depositor

    Tobias von der Haar

    Article

    Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

    Inserts

    Firefly Luciferase
    Renilla luciferase
    

    Expression

    Yeast

    Mutation

    The last three codons of the full-length Firefly …

    Promoter

    ADH1 and TDH3 (=GPD)

    Available Since

    Oct. 22, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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