-
Viral Prep#104488-PHPeBPurposeReady-to-use AAV PHP.eB particles produced from pGP-AAV-syn-jGCaMP7f-WPRE (#104488). In addition to the viral particles, you will also receive purified pGP-AAV-syn-jGCaMP7f-WPRE plasmid DNA. Synapsin-driven GCaMP7f calcium sensor. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsNoneAvailable sinceJuly 29, 2020AvailabilityAcademic Institutions and Nonprofits only
-
pRecT_2
Plasmid#167546PurposeEncodes an IPTG inducible recT from E. faecium Com15 used to ectopically express recT in Enterococcus for recombineering.DepositorInsertE. faecium Com15 recT under an IPTG inducible promoter, with lacI
UseTagsExpressionBacterialMutationPromoterIPTGAvailable sinceJune 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
MSCV IRES Luciferase
Plasmid#18760DepositorTypeEmpty backboneUseLuciferase and RetroviralTagsExpressionMammalianMutationPromoterAvailable sinceJuly 3, 2008AvailabilityAcademic Institutions and Nonprofits only -
T4 Lysozyme WT
Plasmid#18110DepositorInsertT4 Lysozyme (e Bacteriophage T4)
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 26, 2008AvailabilityAcademic Institutions and Nonprofits only -
HDAC1 Flag
Plasmid#13820PurposeMammalian expression of human histone deacetylase 1 with flag tagDepositorInsertHDAC1 (HDAC1 Human)
UseTagsflagExpressionMammalianMutationPromoterAvailable sinceFeb. 19, 2007AvailabilityAcademic Institutions and Nonprofits only -
pENN.AAV.CamKII 0.4.Cre.SV40 (AAV1)
Viral Prep#105558-AAV1PurposeReady-to-use AAV1 particles produced from pENN.AAV.CamKII 0.4.Cre.SV40 (#105558). In addition to the viral particles, you will also receive purified pENN.AAV.CamKII 0.4.Cre.SV40 plasmid DNA. CamKII-driven Cre expression. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCaMKIITagsNoneAvailable sinceMay 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FLAG-LKB1
Plasmid#8590DepositorInsertLKB1 (STK11 Human)
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceJuly 18, 2006AvailabilityAcademic Institutions and Nonprofits only -
dSpCas9-VPR
Plasmid#205247PurposeIn vitro transcription plasmid for production of dSpCas9-VPR mRNA for transcriptional activation (VPR = tripartite transactivator VP64, p65, and Rta). Insert is derived from Addgene plasmid #63798.DepositorInsertdSpCas9-VPR
UseCRISPRTagsExpressionMutationPromoterAvailable sinceSept. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pVITRO1-dV-IgG1/κ
Plasmid#52213PurposeHuman immunoglobulin gamma 1 antibody expression vector (kappa light chain) without variable regions. Enables Colony-PCR screening of false-positive (PCR vector template) coloniesDepositorInsertsHuman immunoglobulin gamma 1 constant region
Human immunoglobulin kappa constant region
UseTagsExpressionMammalianMutationDeleted Variable regionPromoterMouse Elongation Factor 1 Alpha and Rat Elongatio…Available sinceApril 1, 2014AvailabilityAcademic Institutions and Nonprofits only -
MoClo Plant Parts Kit
Plasmid Kit#1000000047PurposeModular Cloning (MoClo) collection contains 95 standardized parts to enable Golden Gate construction of multi-gene constructs for plant transformationDepositorAvailable sinceApril 8, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSLQ5428_pHR_EF1a-mCherry-P2A-Rfx_Cas13d-2xNLS-3xFLAG
Plasmid#155305PurposeLentiviral vector encoding Rfx Cas13d fused with 2xNLS, 3xFLAG, and 2A-tagged mCherry.DepositorInsertmCherry 2A-tagged to Ruminococcus flavefaciens XPD3002 Cas13d
UseLentiviralTags2xNLS-3xFLAGExpressionMammalianMutationPromoterEF1aAvailable sinceJuly 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hsyn-ASAP5-Kv2.1-WPRE
Plasmid#225707PurposePan-neuronal soma-targeted expression of the genetically encoded voltage indicator ASAP5DepositorInsertASAP5-Kv2.1
UseAAVTagsExpressionMutationPromoterhuman synapsinAvailable sinceOct. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCas9
Plasmid#167547PurposeUsed for CRISPR-Cas9 mediated recomineering in Enterococcus. Can clone desired gRNA using BsaI digestion.DepositorInsertschloramphenicol acetyl transferase
pUC19 origin of replication
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceJune 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
DsRed-rab7 WT
Plasmid#12661DepositorInsertRAB7 (Homo sapiens) (RAB7A Human)
UseTagsDsRed and HAExpressionMammalianMutationPromoterAvailable sinceOct. 16, 2006AvailabilityAcademic Institutions and Nonprofits only -
pET-His6-ERK2-MEK1_R4F_coexpression
Plasmid#39212DepositorUseTagsHis6 and RBSExpressionBacterialMutationMEK1(R4F) = S218E, S222D and deletion of AA32-51PromoterT7 and T7 via RBSAvailable sinceJuly 15, 2013AvailabilityAcademic Institutions and Nonprofits only -
pPB-CAG-rtTA-IRES-Hygro
Plasmid#102423PurposeMammalian expression vector for tetracycline-transactivator (rtTA), IRES-HygroDepositorInserttetracyclin-transactivator (rtTA)
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceNov. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-ERK-SPARK
Plasmid#106921PurposeExpress ERK-SPARK, an activity reporter of ERK in mammalian cells.DepositorInsertERK-SPARK
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-EFS-MCP-HALOnls
Plasmid#121937PurposeCRISPR-Sirius plasmidDepositorInsertMCP-HALOnls
UseLentiviralTagsHaloTagExpressionMammalianMutationPromoterEFSAvailable sinceFeb. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
piggyBac-rtTA (4th_Gen)-SNCA(A53T-DNAC)-sfGFP-IRES-NGN2-puro (VK_1124)
Plasmid#209081PurposeNgn2 mediated cortical neuron induction, along with SNCA(A53T)-DeltaNAC-sfGFP over-expressionDepositorUseTagssfGFPExpressionMammalianMutationA53T, NAC deletion (aa60-95)PromoterTRE4GAvailable sinceDec. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pENTR-ERKKTRClover
Plasmid#59138PurposeORF encoding ERK KTR mClover flanked by Gateway sequencesDepositorInsertERK Kinase Translocation Reporter (MAPK1 Human, Mouse)
UseTagsExpressionMutationPromoterAvailable sinceSept. 3, 2014AvailabilityAcademic Institutions and Nonprofits only