161,601 results

1. pCMV-dCas13-M3M14nes

   Plasmid

   #155367

   Purpose

   Targeted m6A RNA methylation in mammalian cells

   Depositor

   David Liu

   Article

   Wilson et al Nat Biotechnol. 2020 Jun 29. pii: 10.103

   Insert

   dCas13-M3M14nes (METTL3, METTL14 Cas13b is from Prevotella sp. P5-125; METTL3 and METTL14 are from H. sapiens)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   PspCas13b Δ984-1090 H133A

   Promoter

   Available since

   July 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. 4xHRE-MinTK-CRE-ODD

   Plasmid

   #141147

   Purpose

   lentiviral expression vector to generate a hypoxia fate-mapping system

   Depositor

   Daniele Gilkes

   Article

   Godet et al Nat Commun. 2019 Oct 24;10(1):4862. doi:

   Insert

   4xHRE-MinTK-CRE-ODD
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   contains an altered Cre gene modified by the addi…

   Promoter

   Available since

   Sept. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. HaloTag-LaminA

   Plasmid

   #231894

   Purpose

   Expression of HaloTag-tagged LaminA to visualize the nuclear lamina

   Depositor

   Lukas Kapitein

   Article

   Korsten et al J Cell Biol. 2024 Nov 4;223(11):e2023071

   Insert

   LaminA (LMNA Human)
   

   Use

   Tags

   HaloTag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti6.2_mNeonGreen2

   Plasmid

   #113727

   Purpose

   Expresses the fluorescent protein mNeonGreen2 in a lentiviral backbone

   Depositor

   Vanessa LaPointe

   Article

   Wieland et al Front Bioeng Biotechnol. 2021 Sep 9;9:72

   Insert

   mNeonGreen2
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pKLV2.2-h7SKgRNA5(SapI)-hU6gRNA5(BbsI)-PGKpuroBFP-W

   Plasmid

   #72666

   Purpose

   Lentiviral dual CRISPR gRNA expression vector (human 7SK and human U6 promoters)

   Depositor

   Kosuke Yusa

   Article

   Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

   Insert

   h7SKgRNA cassette, hU6gRNA cassette, PGKpuroBFP cassette, WPRE
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLenti-hSyn-PROGERIN

   Plasmid

   #140189

   Purpose

   Overexpression of Progerin with a GFP fusion protein, under human synapsin promotor

   Depositor

   Lorenz Studer

   Article

   Miller et al Cell Stem Cell. 2013 Dec 5;13(6):691-705

   Insert

   Progerin (LMNA Human)
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-hSyn-hChR2(H134R)-mCherry (AAV9)

   Viral Prep

   #26976-AAV9

   Purpose

   Ready-to-use AAV9 particles produced from pAAV-hSyn-hChR2(H134R)-mCherry (#26976). In addition to the viral particles, you will also receive purified pAAV-hSyn-hChR2(H134R)-mCherry plasmid DNA. Synapsin-driven, humanized channelrhodopsin H134R mutant, fused to mCherry for optogenetic activation. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Karl Deisseroth

   Promoter

   Syn

   Tags

   mCherry

   Available since

   Jan. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

8. TetO-FUW-OSKM

   Plasmid

   #20321

   Purpose

   Lentiviral plasmid for tet-inducible expression of mouse Oct4, Sox2, Klf4 and Myc for iPS cell generation

   Depositor

   Rudolf Jaenisch

   Article

   Carey et al Proc Natl Acad Sci U S A. 2009 Jan 6. 10

   Insert

   Oct-4 (Pou5f1, Sox2, Klf4, Myc Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 23, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTET GFP10-FRB/FKBP-GFP11

   Plasmid

   #182497

   Purpose

   Tripartite split-GFP. E. coli vector. Inducible coexpression of GFP10-FRB and FKBP-GFP11 coiled coils

   Depositor

   Stéphanie Cabantous

   Article

   Pedelacq et al Methods Mol Biol. 2019;2025:423-437. doi

   Inserts

   FRB
   FKBP (Fkbp1a Synthetic, Mouse)
   

   Use

   Tags

   split GFP10 tripartite and split GFP11 tripartite

   Expression

   Bacterial

   Mutation

   Promoter

   TET

   Available since

   April 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLV-Puro-{CMV-CuO}>{MCS}:T2A:mCherry

    Plasmid

    #236249

    Purpose

    Puromycin selected lentiviral vector with MCS for cumate-inducible gene, for use with dual-inducible CymR-CuO system

    Depositor

    Genevieve Kendall

    Article

    Kent et al Sci Rep. 2024 Sep 5;14(1):20773. doi: 10

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-CuO

    Available since

    June 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. Cytoplasmic PancACe

    Plasmid

    #215708

    Purpose

    Expresses acetyl-CoA biosensor in the cytoplasm

    Depositor

    Katharine Diehl

    Article

    Smith et al Cell Chem Biol. 2025 Jan 21:S2451-9456(2

    Insert

    PancACe
    

    Use

    Lentiviral

    Tags

    FLAG, HA, and cytoplasmic localization sequence

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. FAT FAK biosensor

    Plasmid

    #78303

    Purpose

    FRET biosensor. Visualization of FAK activity at focal adhesions in live cells

    Depositor

    Yingxiao Wang

    Article

    Seong et al Proc Natl Acad Sci U S A. 2013 Nov 26;11

    Insert

    FAT FAK biosensor
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 13, 2016

    Availability

    Academic Institutions and Nonprofits only

13. p6XHis-T7(P266L)

    Plasmid

    #174866

    Purpose

    Expresses a T7 RNA Polymerase that produces long RNA transcripts in high yield and generates fewer abortive transcripts.

    Depositor

    Anna Pyle

    Article

    Chillon et al Methods Enzymol. 2015;558:3-37. doi: 10.

    Insert

    T7 RNA polymerase
    

    Use

    Tags

    6XHis-tag

    Expression

    Bacterial

    Mutation

    P266L

    Promoter

    lac promoter

    Available since

    Nov. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEGFP-Anillin

    Plasmid

    #68027

    Purpose

    Full length Anillin rendered RNAi-resistant) in mammalian expression vector pEGFP-C1

    Depositor

    Michael Glotzer

    Article

    Piekny et al Curr Biol. 2008 Jan 8;18(1):30-6. Epub 2

    Insert

    Anillin (ANLN Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    silent mutations at 798 5'-TGCC TCTTTGAATAAA…

    Promoter

    CMV

    Available since

    Sept. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA5/FRT/TO_Lifeact-HaloTag11-T2A-Tomm20-HaloTag7

    Plasmid

    #175526

    Purpose

    Co-expression of HaloTag11 (actin) and 7 (mitochondrial surface) in mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    Lifeact-HaloTag11 and Tomm20-HaloTag7
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    HaloTag11 = HaloTag7-M175W

    Promoter

    Available since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pC0049-EF1a-dPSPCas13b-NES-HIV, H133A/H1058A

    Plasmid

    #103865

    Purpose

    Catalytically inactive PSPCas13b. RNase activity is inactivated by mutation of catalytic histidine residues in the HEPN domains (H133A/H1058A).

    Depositor

    Feng Zhang

    Article

    Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

    Insert

    PspCas13b
    

    Use

    CRISPR and Lentiviral

    Tags

    3xHA and HIV NES

    Expression

    Mammalian

    Mutation

    Contains the H133A and H1058A mutations which ina…

    Promoter

    EF1a

    Available since

    Jan. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGP-pcDNA3.1 Puro-CAG-Voltron2-ST

    Plasmid

    #172910

    Purpose

    Mammalian expression of voltage sensor; soma localization targeting sequence

    Depositor

    GENIE Project

    Article

    Abdelfattah et al Neuron. 2023 Mar 28:S0896-6273(23)00205-

    Insert

    Voltron2-ST
    

    Use

    Tags

    soma localization targeting sequence

    Expression

    Mammalian

    Mutation

    A122D

    Promoter

    CAG

    Available since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCW-Cas9-2A-EGFP

    Plasmid

    #167928

    Purpose

    Doxycycline-inducible Cas9 expression tracked by EGFP fluorescence marker.

    Depositor

    Ronald Germain

    Article

    Chan et al Immunohorizons. 2021 Feb 23;5(2):117-132

    Insert

    T2A-EGFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    tTRE, hPGK

    Available since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-hSyn1-SIO-eOPN3-mScarlet-WPRE (AAV1)

    Viral Prep

    #125713-AAV1

    Purpose

    Ready-to-use AAV1 particles produced from pAAV-hSyn1-SIO-eOPN3-mScarlet-WPRE (#125713). In addition to the viral particles, you will also receive purified pAAV-hSyn1-SIO-eOPN3-mScarlet-WPRE plasmid DNA. Synapsin-driven, Cre-dependent expression of mosquito OPN3 rhodopsin in-frame with mScarlet.These AAV preparations are suitable purity for injection into animals.

    Depositor

    Ofer Yizhar

    Article

    Mahn et al Neuron. 2021 May 10. pii: S0896-6273(21)

    Promoter

    Syn

    Tags

    mScarlet (Cre-dependent)

    Available since

    Sept. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

20. PiggyBac dCas9-KRAB

    Plasmid

    #191314

    Purpose

    piggyBac integration and dox-inducible expression of KRAB-dCas9-HA-tagBFP

    Depositor

    Joanna Wysocka

    Article

    Fuentes et al Elife. 2018 Aug 2;7. pii: 35989. doi: 10

    Inserts

    KRAB-dCas9-HA-tagBFP
    rtTA3-IRES-PURO
    

    Use

    CRISPR

    Tags

    HA and tagBFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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