165,810 results

1. YIp204-PADH1-atTIR1-9myc

   Plasmid

   #99532

   Purpose

   S. cerevisiae expression of F-box protein TIR1-9myc under control of the ADH1 promoter, TRP1 marker (for use with the AID degron system)

   Depositor

   Helle Ulrich

   Insert

   TIR1 (TIR1 Mustard Weed)
   

   Use

   Tags

   9xMyc

   Expression

   Yeast

   Mutation

   Promoter

   ADH1

   Available Since

   Sept. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEN366 - pTRE3G-CTCF-mRuby2-BGHpA-CAGGS-rtta3G-rbgpA-Frt-PGK-EM7-PuroR-bpA-Frt TIGRE donor

   Plasmid

   #156432

   Purpose

   Vector to introduce a constitutive rtta3G cassette and a DOX-inducible wild-type CTCF mouse cDNA, targeting the mouse TIGRE acceptor locus. Use with Addgene #92144 sgRNA. Use puromycine selection.

   Depositor

   Elphege Nora

   Article

   Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

   Insert

   CTCF, rtta3G, TetO3G (Ctcf Mouse)
   

   Use

   Mouse Targeting

   Tags

   Expression

   Mammalian

   Mutation

   none

   Promoter

   Available Since

   Sept. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-CaMKII-WHaloCaMP1a-EGFP

   Plasmid

   #205309

   Purpose

   Production of AAV particles encoding WHaloCaMP1a-EGFP from the CaMKII promoter

   Depositor

   Eric Schreiter

   Article

   Farrants et al bioRxiv. 2023 Jul 19:2023.07.18.549527.

   Insert

   WHaloCaMP1a-EGFP
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Aug. 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti_hU6-peg(ATP1A1-T804N)_mU6-BsmBI_Puro

   Plasmid

   #234070

   Purpose

   For lentiviral expression of user-specified pegRNA in tandem with epegRNA (ATP1A1-T804N) for ouabain co-selection

   Depositor

   Greg Findlay

   Article

   Herger et al Cell Genom. 2025 Mar 18:100814. doi: 10.

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   March 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMSP1E3D1_D73C

   Plasmid

   #39328

   Depositor

   Stephen Sligar

   Article

   Ritchie et al Methods Enzymol. 2009;464:211-31. doi: 1

   Insert

   MSP1E3D1_D73C (APOA1 synthetic gene)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   cys mutant of MSP1E3D1

   Promoter

   T7

   Available Since

   Sept. 11, 2012

   Availability

   Academic Institutions and Nonprofits only

6. pSH-CAG-AtAFB2.F74A-IRES-puro

   Plasmid

   #216245

   Purpose

   Express AtAFB2.F74A mutant without tag for AID2

   Depositor

   Elina Ikonen

   Article

   Li et al Genome Biol. 2024 Feb 26;25(1):58. doi:

   Insert

   AtAFB2.F74A
   

   Use

   CRISPR and TALEN; Human safe harbor locus (a avs1…

   Tags

   Expression

   Mammalian

   Mutation

   F74A mutation

   Promoter

   CAG

   Available Since

   March 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. Lenti-Cre

   Plasmid

   #198712

   Purpose

   For lentiviral expression of Cre recombinase in mammalian cells.

   Depositor

   Tyler Jacks

   Article

   Gaglia et al Cancer Cell. 2023 May 8;41(5):871-886.e1

   Insert

   Pgk-Cre
   

   Use

   Cre/Lox and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PGK

   Available Since

   Jan. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBR322-PVCpnf17-22

   Plasmid

   #198272

   Purpose

   Unmodified pPayload; contains WT PVC payloads/regulatory genes

   Depositor

   Feng Zhang

   Article

   Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

   Insert

   PVCpnf17-22
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   March 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pACAGW-ChR2-Venus-AAV (AAV9)

   Viral Prep

   #20071-AAV9

   Purpose

   Ready-to-use AAV9 particles produced from pACAGW-ChR2-Venus-AAV (#20071). In addition to the viral particles, you will also receive purified pACAGW-ChR2-Venus-AAV plasmid DNA. CAG-driven, channelrhodopsin fused to Venus for optogenetic activation. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Karel Svoboda

   Article

   Leopoldo et al Nature. 2009 Jan 18.

   Promoter

   CAGG

   Tags

   Venus

   Available Since

   March 15, 2018

   Availability

   Academic Institutions and Nonprofits only

10. PIEZO1-HaloTag

    Plasmid

    #207834

    Purpose

    PIEZO1 fusion for biochemistry and live imaging

    Depositor

    Charles Cox

    Article

    Zhou et al Science. 2023 Aug 18;381(6659):799-804.

    Insert

    PIEZO1 (PIEZO1 Human)
    

    Use

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Nov. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCaggs-mGold2t

    Plasmid

    #231764

    Purpose

    Expression of mGold2t in mammalian cells

    Depositor

    Francois St-Pierre

    Article

    Lee et al Nat Commun. 2025 Apr 4;16(1):3241. doi:

    Insert

    mGold2t
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    mGold2t is mGold with V1A;V22I;H77N;Q80R;K101E;D1…

    Promoter

    Actin promoter with CMV enhancer

    Available Since

    April 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1-mBATF

    Plasmid

    #34575

    Depositor

    Peter Murray

    Article

    Smith et al J Biol Chem. 2011 Jul 1;286(26):23582-90

    Insert

    BATF (Batf Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Dec. 16, 2011

    Availability

    Academic Institutions and Nonprofits only

13. pGL3-SEC61B.N-BSD.P2A.miniIAA7.3xFlag

    Plasmid

    #216250

    Purpose

    HDR template to tag endogenous human SEC61B N-terminus with BSD.P2A.miniIAA7.3xFlag

    Depositor

    Elina Ikonen

    Article

    Li et al Genome Biol. 2024 Feb 26;25(1):58. doi:

    Insert

    HDR template for human SEC61B (SEC61B HDR template, Human, Synthetic)
    

    Use

    CRISPR; Hdr template

    Tags

    BSD.P2A.miniIAA7.3xFlag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1(+)IRES GFP

    Plasmid

    #51406

    Purpose

    Expresses GFP from internal ribosome entry sequence, cloning component

    Depositor

    Kathleen_L Collins

    Article

    Schaefer et al J Immunol. 2008 Jun 15;180(12):7804-17.

    Insert

    IRES GFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGP-AAV-syn-jGCaMP8s-WPRE

    Plasmid

    #162374

    Purpose

    AAV-mediated expression of ultrafast protein calcium sensor under the Syn promoter

    Depositor

    GENIE Project

    Article

    Zhang et al Janelia Research Campus, Online Resource

    Has Service

    AAV Retrograde, AAV1, AAV5, and AAV9

    Insert

    jGCaMP8s
    

    Use

    AAV

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    S26M F286Y Q315H

    Promoter

    Synapsin

    Available Since

    Nov. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. Cre-IRES-PuroR

    Plasmid

    #30205

    Purpose

    Lentiviral coexpression of Cre and puromycin from the human EF1a promoter

    Depositor

    Darrell Kotton

    Article

    Somers et al Stem Cells. 2010 Oct . 28(10):1728-40.

    Insert

    Cre recombinase (cre Enterobacteria phage P1)
    

    Use

    Cre/Lox and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available Since

    April 12, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV-T7-TadCBEd-SpRY-P2A-EGFP (BKS428)

    Plasmid

    #223125

    Purpose

    pCMV and pT7 Human expression plasmid for TadCBEd-SpRY with C-term BPNLS-P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    human codon optimized TadCBEd-SpRY-2xUGI-P2A-EGFP
    

    Use

    CRISPR

    Tags

    2xUGI-BPNLS-P2A-EGFP and BPNLS-TadA-CDd

    Expression

    Mammalian

    Mutation

    TadA-CDd mutations; nSpRY=D10A/A61R/L1111R/D1135L…

    Promoter

    CMV and T7

    Available Since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. Lenti-human-TF-gRNA-puromycin

    Pooled Library

    #162275

    Purpose

    This is a gRNA library targeting on ~1600 human transcription factors for genetic screening.

    Depositor

    Chunliang Li

    Article

    Zhang et al Elife. 2020 Oct 1;9. pii: 57858. doi: 10

    Species

    Homo sapiens

    Use

    Lentiviral

    Available Since

    Dec. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

19. pSH-CAG-AtAFB2.F74A-mCherry-IRES-puro

    Plasmid

    #216244

    Purpose

    Express AtAFB2.F74A mutant with mCherry tag for AID2

    Depositor

    Elina Ikonen

    Article

    Li et al Genome Biol. 2024 Feb 26;25(1):58. doi:

    Insert

    AtAFB2.F74A-mCherry
    

    Use

    CRISPR, TALEN, and Unspecified ; Human safe harbo…

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    F74A mutation

    Promoter

    CAG

    Available Since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. AiP12918 - pAAV-AiE0459m_3xC2-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2918)

    Plasmid

    #224016

    Purpose

    AiE0459m_3xC2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in cortical L5_ET neurons

    Depositor

    The Allen Institute for Brain Science, Jonathan Ting

    Article

    Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

    Insert

    SYFP2
    

    Use

    AAV

    Tags

    Expression

    Mutation

    NA

    Promoter

    minBG

    Available Since

    Oct. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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