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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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163,819 results

Showing: 2501 - 2520 of 163819 results

  1. BPK1520

    Plasmid
    #65777
    Purpose
    Human expression plasmid for SpCas9 sgRNA (need to clone in spacer into BsmBI sites): U6-BsmBIcassette-Sp-sgRNA
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature
    Insert
    SpCas9 gRNA backbone, without spacer sequence
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    June 22, 2015
    Availability
    Academic Institutions and Nonprofits only
    65777-plasmid-map-sequence-id-316938

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  5. pAAV-Syn-ChrimsonR-tdT (AAV9)

    Viral Prep
    #59171-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pAAV-Syn-ChrimsonR-tdT (#59171). In addition to the viral particles, you will also receive purified pAAV-Syn-ChrimsonR-tdT plasmid DNA. Syn-driven ChrimsonR-tdTomato expression for optogenetic neural activation. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Edward Boyden
    Article
    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:
    Promoter
    Syn
    Tags
    tdTomato
    Available since
    Feb. 7, 2018
    Availability
    Academic Institutions and Nonprofits only

  8. pEM040 [Multiome Perturb-seq sgRNA expression vector]

    Plasmid
    #224898
    Purpose
    Lentiviral CROP-seq vector with lineage barcode cassette and T7 promoter for ID amplification from cDNA, engineered for high-efficiency ID assignment in Multiome Perturb-seq experiments
    Depositor
    Thomas Norman
    Article
    Metzner et al bioRxiv [Preprint]. 2024 Jul 27:2024.07.
    Inserts
    eGFP
    LacZ fragment (removed by digestion with BstXI/BlpI for sgRNA cloning)
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1a
    Available since
    Aug. 28, 2024
    Availability
    Academic Institutions and Nonprofits only
    224898-plasmid-map-sequence-id-443622

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  12. pAAV.hSyn.iGluSnFR3.v857.GPI (AAV1)

    Viral Prep
    #178331-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV.hSyn.iGluSnFR3.v857.GPI (#178331). In addition to the viral particles, you will also receive purified pAAV.hSyn.iGluSnFR3.v857.GPI plasmid DNA. Synapsin-driven, third generation glutamate sensor iGluSnFR3, variant 857. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Kaspar Podgorski
    Article
    Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4
    Promoter
    Tags
    None
    Available since
    May 16, 2023
    Availability
    Academic Institutions and Nonprofits only

  13. TFORF3081

    Plasmid
    #144557
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    ELK3 (ELK3 Human)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Aug. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    144557-plasmid-map-sequence-id-404250

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  14. pcDNA-dCas9-FLp300

    Plasmid
    #61356
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of FL human p300 (aa 2-2414) driven by CMV promoter
    Depositor
    Charles Gersbach
    Article
    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do
    Insert
    S.pyogenes dCas9 with c-terminal full length human p300 (aa 2-2414) (EP300 S. pyogenes, Human, Synthetic)
    Use
    CRISPR and Synthetic Biology
    Tags
    Flag, HA, and SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9
    Promoter
    CMV
    Available since
    April 16, 2015
    Availability
    Academic Institutions and Nonprofits only
    61356-plasmid-map-sequence-id-99784

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  16. pcDNA-RLuc8

    Plasmid
    #87121
    Purpose
    Mammalian expression vector expressing mutant version of the reporter gene-Renilla Luciferase (Rluc8), which is more stable in mouse serum and has more light output.
    Depositor
    Sanjiv Sam Gambhir
    Article
    Loening et al Protein Eng Des Sel. 2006 Sep;19(9):391-
    Insert
    Renilla Luciferase
    Use
    Luciferase
    Tags
    Expression
    Mammalian
    Mutation
    A55T, C124A, S130A, K136R, A143M, M185V, M253L, a…
    Promoter
    CMV
    Available since
    April 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    87121-plasmid-map-sequence-id-175252

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  18. pAAV-FLEX-GFP

    Plasmid
    #28304
    Depositor
    Edward Boyden
    Insert
    GFP
    Use
    AAV and Cre/Lox; Adeno-associated virus
    Tags
    Expression
    Mammalian
    Mutation
    N/A
    Promoter
    CAG
    Available since
    July 7, 2011
    Availability
    Academic Institutions and Nonprofits only
    28304-plasmid-map-sequence-id-454424

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  20. pAAV-CaMKIIa-hM4D(Gi)-mCherry (AAV9)

    Viral Prep
    #50477-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pAAV-CaMKIIa-hM4D(Gi)-mCherry (#50477). In addition to the viral particles, you will also receive purified pAAV-CaMKIIa-hM4D(Gi)-mCherry plasmid DNA. CaMKIIa-driven hM4D(Gi) receptor with an mCherry reporter for CNO-induced neuronal silencing. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Bryan Roth
    Promoter
    CaMKII
    Tags
    mCherry
    Available since
    Jan. 26, 2021
    Availability
    Academic Institutions and Nonprofits only
Showing: 2501 - 2520 of 163819 results