|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||32701||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5428
Modifications to backbonenone
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Copy numberHigh Copy
SpeciesH. sapiens (human)
Insert Size (bp)1950
Entrez GeneHSPA5 (a.k.a. BIP, GRP78, HEL-S-89n)
- Promoter CMV
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (not destroyed)
- 3′ cloning site XHOI (not destroyed)
- 5′ sequencing primer T7 (Common Sequencing Primers)
The D238G point mutation found in the Addgene sequence is a known variation in human GRP78 that does not alter its chaperone or ATPase activities.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pcDNA3.1(+)-GRP78/BiP was a gift from Richard C. Austin (Addgene plasmid # 32701 ; http://n2t.net/addgene:32701 ; RRID:Addgene_32701)
For your References section:Homocysteine-induced endoplasmic reticulum stress causes dysregulation of the cholesterol and triglyceride biosynthetic pathways. Werstuck GH, Lentz SR, Dayal S, Hossain GS, Sood SK, Shi YY, Zhou J, Maeda N, Krisans SK, Malinow MR, Austin RC. J Clin Invest. 2001 May;107(10):1263-73. 10.1172/JCI11596 PubMed 11375416