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pCMV BiP-Myc-KDEL-wt
(Plasmid #27164)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 27164 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    BiP
  • Species
    H. sapiens (human)
  • GenBank ID
    NP_005338.1
  • Entrez Gene
    HSPA5 (a.k.a. BIP, GRP78, HEL-S-89n)
  • Tags / Fusion Proteins
    • Myc (C terminal on insert)
    • KDEL (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (unknown if destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer CMV-F
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Articles Citing this Plasmid
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV BiP-Myc-KDEL-wt was a gift from Ron Prywes (Addgene plasmid # 27164 ; http://n2t.net/addgene:27164 ; RRID:Addgene_27164)
  • For your References section:

    ER stress regulation of ATF6 localization by dissociation of BiP/GRP78 binding and unmasking of Golgi localization signals. Shen J, Chen X, Hendershot L, Prywes R. Dev Cell. 2002 Jul . 3(1):99-111. 10.1016/S1534-5807(02)00203-4 PubMed 12110171