PurposeFor mammalian expression of human ATF6 with a 3x FLAG tag
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||11975||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4700
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
SpeciesH. sapiens (human)
Entrez GeneATF6 (a.k.a. ACHM7, ATF6A)
/ Fusion Protein
- 3xFLAG (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (not destroyed)
- 3′ cloning site SalI (not destroyed)
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
See author's map for cloning details.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:p3xFLAG-ATF6 was a gift from Ron Prywes (Addgene plasmid # 11975 ; http://n2t.net/addgene:11975 ; RRID:Addgene_11975)
For your References section:The luminal domain of ATF6 senses endoplasmic reticulum (ER) stress and causes translocation of ATF6 from the ER to the Golgi. Chen X, Shen J, Prywes R. J Biol Chem. 2002 Apr 12. 277(15):13045-52. 10.1074/jbc.M110636200 PubMed 11821395