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The luminal domain of ATF6 senses endoplasmic reticulum (ER) stress and causes translocation of ATF6 from the ER to the Golgi.
Chen X, Shen J, Prywes R
J Biol Chem. 2002 Apr 12. 277(15):13045-52.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
11975p3xFLAG-ATF6For mammalian expression of human ATF6 with a 3x FLAG tag
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32955pEGFP-ATF6For visualization of GFP-ATF6 in mammalian cells.
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32956pEGFP-ATF6-(S1P-)For visualization of ATF6 in mammalian cells with a mutation in ATF6 that blocks cleavage by protease S1P. Activated protein will remain in the Golgi.
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Antibodies from Article