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(Plasmid #21833)


This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 21833 Standard format: Plasmid sent in bacteria as agar stab 1 $85


  • Vector backbone
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number


  • Gene/Insert name
    XBP-1 processed/spliced
  • Alt name
  • Alt name
    X-box binding protein 1
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Xbp1 (a.k.a. D11Ertd39e, TREB-5, TREB5, XBP-1)
  • Tag / Fusion Protein
    • Flag (N terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Addgene sequencing results show two mismatches with author's sequence causing two amino acid mutations - G26V and A76V.

FLAG-epitope tagged IRE1-spliced XBP-1 in the mammalian expression plasmid pFLAG-CMV-2. Flag-tagged version of XBP-1 was constructed by inserting the Flag epitope tag sequence immediately 3' of the ATG start codon.

Note: Cheah Kathryn Song Eng reported on November 4, 2004 that the sequence of this plasmid deviates from the predicted by G1028T. This impacts on the CDS changing Gly27Val. We do not know if this is a mutation that crept into our construction, a mutation that was present in the cDNA we purchased or if it is a point in which the mouse genome has some variability. Nor do we know the functional significance of this, but we note that the corresponding residue in the human is Ala. Furthermore, this variation/mutation is in a region of relatively poor sequence conservation across species, suggesting that it might not be all that important.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    XBP-1 2: pFLAG.XBP1p.CMV2 was a gift from David Ron (Addgene plasmid # 21833 ; ; RRID:Addgene_21833)
  • For your References section:

    IRE1 couples endoplasmic reticulum load to secretory capacity by processing the XBP-1 mRNA. Calfon M, Zeng H, Urano F, Till JH, Hubbard SR, Harding HP, Clark SG, Ron D. Nature. 2002 Jan 3. 415(6867):92-6. 10.1038/415092a PubMed 11780124