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pFLAG-XBP1u-FLuc
(Plasmid #31239)

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 31239 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pTriEx-4
  • Modifications to backbone
    Renilla luciferase and the 5′ untranslated region (UTR) containing IRES of EV71 is included upstream of the FLAG-XBP1u-FLuc insert.
  • Vector type
    Mammalian Expression, Bacterial Expression, Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    FLAG-XBP1u-FLuc
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1700
  • Tags / Fusion Proteins
    • Flag (N terminal on insert)
    • Fluc (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Xho1 (not destroyed)
  • 3′ cloning site Xho1 (not destroyed)
  • 5′ sequencing primer TriExUP primer
  • 3′ sequencing primer TriExDOWN primer
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

EV71 5′ UTR between Renilla and firefly luciferase.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFLAG-XBP1u-FLuc was a gift from Jimtong Horng (Addgene plasmid # 31239 ; http://n2t.net/addgene:31239 ; RRID:Addgene_31239)
  • For your References section:

    Endoplasmic reticulum stress is induced and modulated by enterovirus 71. Jheng JR, Lau KS, Tang WF, Wu MS, Horng JT. Cell Microbiol. 2010 Jun . 12(6):796-813. 10.1111/j.1462-5822.2010.01434.x PubMed 20070307