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Depositing Lab
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Depositing OrganizationChang Gung University
Located in Taiwan -
Publication
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Citations
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 31239 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 31239-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 31239-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepTriEx-4
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Modifications to backboneRenilla luciferase and the 5′ untranslated region (UTR) containing IRES of EV71 is included upstream of the FLAG-XBP1u-FLuc insert.
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Vector typeMammalian Expression, Bacterial Expression, Insect Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameFLAG-XBP1u-FLuc
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SpeciesH. sapiens (human)
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Insert Size (bp)1700
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Entrez GeneXBP1 (a.k.a. TREB-5, TREB5, XBP-1, XBP2)
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Tags
/ Fusion Proteins
- Flag (N terminal on insert)
- Fluc (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Xho1 (not destroyed)
- 3′ cloning site Xho1 (not destroyed)
- 5′ sequencing primer TriExUP primer
- 3′ sequencing primer TriExDOWN primer
- (Common Sequencing Primers)
Resource Information
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Addgene Notes
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
EV71 5′ UTR between Renilla and firefly luciferase.
DNA (Catalog # 31239-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 31239-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pFLAG-XBP1u-FLuc was a gift from Jimtong Horng (Addgene plasmid # 31239 ; http://n2t.net/addgene:31239 ; RRID:Addgene_31239) -
For your References section:
Endoplasmic reticulum stress is induced and modulated by enterovirus 71. Jheng JR, Lau KS, Tang WF, Wu MS, Horng JT. Cell Microbiol. 2010 Jun . 12(6):796-813. 10.1111/j.1462-5822.2010.01434.x PubMed 20070307
Ready-to-use antibodies
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Learn MoreMap uploaded by the depositor.
