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(Plasmid #62233)


This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 62233 Standard format: Plasmid sent in bacteria as agar stab 1 $85


  • Vector backbone
    clontech N1
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 4000
  • Total vector size (bp) 6700
  • Vector type
    Mammalian Expression
  • Selectable markers

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
    hamster BiP-mCherry-KDEL
  • Species
    Synthetic; hamster
  • Insert Size (bp)
  • Promoter cmv
  • Tag / Fusion Protein
    • mCherry (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Nhe1 (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer tggtttagtgaaccgt
  • 3′ sequencing primer GTTCAGGGGGAGGTGTG
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Avoid overexpression. Low expressing cells are best for imaging. High expression leads to protein aggregation in the nuclear envelope. See supplementary data for original paper.

Do NOT use this as an ER marker. BiP is a functional protein and significantly impacts the ER environment increasing stress resistance. Use ER-mCherry if you simply want a marker for the ER when imaging cells.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    BiP-mCherry was a gift from Erik Snapp (Addgene plasmid # 62233 ; ; RRID:Addgene_62233)
  • For your References section:

    BiP availability distinguishes states of homeostasis and stress in the endoplasmic reticulum of living cells. Lai CW, Aronson DE, Snapp EL. Mol Biol Cell. 2010 Jun 15;21(12):1909-21. doi: 10.1091/mbc.E09-12-1066. Epub 2010 Apr 21. 10.1091/mbc.e09-12-1066 PubMed 20410136