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Purposevisualization of the ER
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Depositing Lab
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Depositing OrganizationUniversity of Colorado, Boulder
Located in United States of America -
Citations
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 49150 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 49150-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 49150-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepTagBFP-C
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Backbone manufacturerEvrogen
- Backbone size w/o insert (bp) 4707
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameBiP ER signal sequence
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SpeciesH. sapiens (human)
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Entrez GeneHSPA5 (a.k.a. BIP, GRP78, HEL-S-89n)
- Promoter CMV
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Tags
/ Fusion Proteins
- TagBFP (C terminal on backbone)
- KDEL (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NheI (not destroyed)
- 3′ cloning site KpnI (not destroyed)
- 5′ sequencing primer CMV-F
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 49150-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 49150-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
BFP-KDEL was a gift from Gia Voeltz (Addgene plasmid # 49150 ; http://n2t.net/addgene:49150 ; RRID:Addgene_49150) -
For your References section:
ER tubules mark sites of mitochondrial division. Friedman JR, Lackner LL, West M, DiBenedetto JR, Nunnari J, Voeltz GK. Science. 2011 Oct 21;334(6054):358-62. doi: 10.1126/science.1207385. Epub 2011 Sep 1. 10.1126/science.1207385 PubMed 21885730
