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pLenti-CMV-MCS-GFP-SV-puro
(Plasmid #73582)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 73582 Standard format: Plasmid sent in bacteria as agar stab 1 $75
Cloning Grade DNA 73582-DNA.cg 2 µg of cloning grade DNA in Tris buffer 1 $95

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pLenti-CMV-MCs-SV-puro
  • Backbone manufacturer
    gift from Dr. Hong Zhang, University of Massachusetts Medical School
  • Backbone size (bp) 8829
  • Modifications to backbone
    GFP insert
  • Vector type
    Lentiviral
  • Selectable markers
    Puromycin
  • Tag / Fusion Protein
    • GFP tag (C terminal on backbone)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Cloning Information

Resource Information

Information for Cloning Grade DNA (Catalog # 73582-DNA.cg) ( Back to top )

Purpose

Cloning grade DNA is suitable for use in PCR, cloning reactions, or transformation into E. coli. The purity and amount is not suitable for direct transfections.

Delivery

  • Amount 2 µg
  • Guaranteed Concentration 100 ng/µl +/- 5 ng/µl
  • Pricing $95 USD
  • Storage DNA can be stored at 4℃ (short term) or -20℃ (long term).

Resource Information

Quality Control

Addgene has verified this plasmid using Next Generation Sequencing. Results are available here

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLenti-CMV-MCS-GFP-SV-puro was a gift from Paul Odgren (Addgene plasmid # 73582 ; http://n2t.net/addgene:73582 ; RRID:Addgene_73582)
  • For your References section:

    Studies of OC-STAMP in Osteoclast Fusion: A New Knockout Mouse Model, Rescue of Cell Fusion, and Transmembrane Topology. Witwicka H, Hwang SY, Reyes-Gutierrez P, Jia H, Odgren PE, Donahue LR, Birnbaum MJ, Odgren PR. PLoS One. 2015 Jun 4;10(6):e0128275. doi: 10.1371/journal.pone.0128275. eCollection 2015. PONE-D-14-50827 [pii] PubMed 26042409