430 results

#220921

cssDNA production strain

Shawn Douglas

Shen et al Nucleic Acids Res. 2024 Apr 24;52(7):409

Aug. 19, 2024

Academic Institutions and Nonprofits only

#49018

This is C321.DA with mutS reverted and the lambda prophage removed; it exhibits lesser spontaneous mutagenesis and can be grown at 37 C

George Church

Lajoie et al Science. 2013 Oct 18;342(6156):357-60. d

Feb. 18, 2014

Academic Institutions and Nonprofits only

#182776

Recoded E. coli MG1655 strain with UAG termination function removed (RF1 is deleted); T7RNAP cassette genomically inserted

Michael Jewett

Perez et al Biotechnol J. 2021 Dec 11:e2100330. doi:

April 18, 2023

Academic Institutions and Nonprofits only

#189857

Laboratory adapted, genomically recoded E. coli strain without TCG, TCA, or TAG codons and deleted serT, serU, prfA, and recA genes. Evolved for ~270 generations in LB at 37 °C.

George Church

Nyerges et al Nature. 2023 Mar;615(7953):720-727. doi:

Jan. 27, 2023

Academic Institutions and Nonprofits only

#174513

Recoded E. coli strain without TCG, TCA, or TAG codons in all open reading frames. Full sequence - GenBank: CP040347.1

Jason W Chin

Fredens et al Nature. 2019 May;569(7757):514-518. doi:

Nov. 18, 2021

Academic Institutions and Nonprofits only

#61440

This E. coli chassis displays reduced endogenous reduction of aromatic and aliphatic aldehydes

Kristala Prather

Kunjapur et al J Am Chem Soc. 2014 Aug 20;136(33):11644

Feb. 1, 2015

Academic Institutions and Nonprofits only

#248173

Maintenance of R6K plasmids at 13 PCN

Thomas Gorochowski

Hina-Nilesh Joshi et al ACS Synth Biol. 2026 Jan 13. doi: 10.102

March 9, 2026

Academic Institutions and Nonprofits only

#197132

Deficient in the packaging cos site and encoding rhamnose-inducible P4 delta and epsilon genes. It also has tail fiber (H) and its chaperone (G) deleted.

Baojun Wang

Fa-Arun et al ACS Synth Biol. 2023 Feb 17;12(2):596-60

July 11, 2023

Academic Institutions and Nonprofits only

#209018

Deficient in the P2 bacteriophage packaging cos site and encoding rhamnose-inducible P4 delta (δ) and epsilon (ε) genes

Alfonso Jaramillo

Tridgett et al ACS Synth Biol. 2021 Jan 15;10(1):107-11

Jan. 17, 2025

Academic Institutions and Nonprofits only

#115925

Expression of dCas9 gene under the control of a weak Ptet promoter in the E. coli chromosome

David Bikard

Cui et al Nat Commun. 2018 May 15;9(1):1912. doi:

March 26, 2019

Academic Institutions and Nonprofits only

#52706

KIlac with lacZ deletion

Tanja Kortemme

Eames et al Science. 2012 May 18;336(6083):911-5. do

Feb. 2, 2015

Academic Institutions and Nonprofits only

#115924

Expression of dCas9 gene under the control of a Ptet promoter in the E. coli chromosome

David Bikard

Cui et al Nat Commun. 2018 May 15;9(1):1912. doi:

March 26, 2019

Academic Institutions and Nonprofits only

#26242

Phage-resistant derivative of BL21(DE3), which has been transformed with plasmid pRARE2, which carries seven rare-codon tRNA genes.

Opher Gileadi

Savitsky et al J Struct Biol. 2010 Oct;172(1):3-13. doi

Sept. 6, 2019

Academic Institutions and Nonprofits only

#197934

BL21(DE3)-based E. coli with 95 UAG codons and RF-1 being removed for translating UAG to noncanonical amino acids

Kensaku Sakamoto

Mukai et al Sci Rep. 2015 May 18;5:9699. doi: 10.103

March 8, 2023

Academic Institutions and Nonprofits only

#229395

Escherichia coli DH10B DAP-auxotrophic strain containing an integrated RK2/RP4 mobilization vector to conjugate plasmids with an IncP origin of transfer (modified pTA-Mob 2.0, AddGene Plasmid #149662)

Michael Springer

Soltysiak et al ACS Synth Biol. 2025 Apr 18;14(4):1173-1

March 24, 2025

Academic Institutions and Nonprofits only

#176581

E. coli BL21 strain (with recBCD genomic locus deleted) for making cell-free extracts for Linear DNA expression.

Chase Beisel, Jean-Loup Faulon

Batista et al ACS Synth Biol. 2022 Jan 16. doi: 10.102

Dec. 14, 2021

Academic Institutions and Nonprofits only

#99247

LacZ deficient (lac operon deletion) BL21-Gold-dLac (DE3) strain

Jeff Hasty

Didovyk et al ACS Synth Biol. 2017 Aug 10. doi: 10.102

Nov. 1, 2017

Academic Institutions and Nonprofits only

#98417

Bacterial Strain with Genotype: MC4100 ∆clpPX

Johan Paulsson

Potvin-Trottier et al Nature. 2016 Oct 12;538(7626):514-517. d

July 10, 2017

Academic Institutions and Nonprofits only

#62077

BL21(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. trpA trpB genes deleted.

Robert Gennis, Toshio Iwasaki

Lin et al Methods Enzymol. 2015;565:45-66. doi: 10

Jan. 26, 2015

Academic Institutions and Nonprofits only

#155206

SQ171 strain supported by pCSacB and ptRNA100 plasmids

Alexander Mankin

Huang et al Nat Commun. 2020 Jun 9;11(1):2900. doi:

April 27, 2021

Academic Institutions and Nonprofits only