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Han Lim Lab Plasmids


The Han Lim Lab has deposited plasmids at Addgene for distribution to the research community. Addgene is a nonprofit plasmid repository dedicated to improving life science research.

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Title Authors Publication
A multistep epigenetic switch enables the stable inheritance of DNA methylation states. Lim HN, van Oudenaarden A Nat Genet. 2007 Feb . 39(2):269-75.
Disruption of small RNA signaling caused by competition for Hfq. Hussein R, Lim HN Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28.
Han Lim Lab Strains (unpublished)
Minimal effect of gene clustering on expression in Escherichia coli. Liang LW, Hussein R, Block DH, Lim HN Genetics. 2013 Feb;193(2):453-65. doi: 10.1534/genetics.112.147199. Epub 2012 Dec 5.
Fundamental relationship between operon organization and gene expression. Lim HN, Lee Y, Hussein R Proc Natl Acad Sci U S A. 2011 Jun 28;108(26):10626-31. doi: 10.1073/pnas.1105692108. Epub 2011 Jun 13.
Rapid and robust signaling in the CsrA cascade via RNA-protein interactions and feedback regulation. Adamson DN, Lim HN Proc Natl Acad Sci U S A. 2013 Aug 6;110(32):13120-5. doi: 10.1073/pnas.1308476110. Epub 2013 Jul 22.
Direct comparison of small RNA and transcription factor signaling. Hussein R, Lim HN Nucleic Acids Res. 2012 May 22.
Lim Lab unpublished plasmids (csrA crosstalk) (unpublished)
Modulating the frequency and bias of stochastic switching to control phenotypic variation. Hung M, Chang E, Hussein R, Frazier K, Shin JE, Sagawa S, Lim HN Nat Commun. 2014 Aug 4;5:4574. doi: 10.1038/ncomms5574.
Lim Lab plasmids (unpublished)
Regulatory consequences of gene translocation in bacteria. Block DH, Hussein R, Liang LW, Lim HN Nucleic Acids Res. 2012 Oct;40(18):8979-92. doi: 10.1093/nar/gks694. Epub 2012 Jul 24.
Paradoxical suppression of small RNA activity at high Hfq concentrations due to random-order binding. Sagawa S, Shin JE, Hussein R, Lim HN Nucleic Acids Res. 2015 Aug 10. pii: gkv777.
Quantitative characterization of gene regulation by Rho dependent transcription termination. Hussein R, Lee TY, Lim HN Biochim Biophys Acta. 2015 May 14;1849(8):940-954. doi: 10.1016/j.bbagrm.2015.05.003.
Horizontal transfer of DNA methylation patterns into bacterial chromosomes. Shin JE, Lin C, Lim HN Nucleic Acids Res. 2016 Apr 15. pii: gkw230.
ID Plasmid Gene/Insert Vector Type Publication Hidden Extra Search Info  
24387pHL32 (in cHNL135)Bacterial Expression, Synthetic BiologyA multistep epigenetic switch enables the stable inheritance of DNA methylation states. Nat Genet. 2007 Feb . 39(2):269-75. pHL32 Add to Cart
30006HL 818 (=HL 716 + pHL 98)pLlacO-1:DsrA-mcherry, pLtetO-1:rpoS::gfp (Other)Bacterial Expression, Synthetic BiologyDisruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28. colE (from pZ system) + AmpR Add to Cart
30008HL 839 (=HL 716 + pHL 108)pLlacO-1:MicC-mCherry, pLtetO-1:ompC::gfp (Other)Bacterial Expression, Synthetic BiologyDisruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28. ColE (from pZ system) + AmpR Add to Cart
30009HL 849 (=HL 716 + micC knockout_KanR)Synthetic BiologyDisruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28. Unknown Add to Cart
30012HL 1178 (=HL 862 + pHL 282)pLtetO-1:mCherry, pLlacO-1:ompC::gfpBacterial Expression, Synthetic BiologyDisruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28. ColE (from pZ system) + AmpR Add to Cart
30014HL 1329 (=HL 865 + pHL 344)pLtetO-1:mCherry, pLlacO-1:rpoS::gfpBacterial Expression, Synthetic BiologyDisruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28. ColE (from pZ system) + AmpR Add to Cart
30015HL 1474 (=HL 862 + pHL 404)pLtetO-1:MicC, pLlacO-1:hfq, pLtetO-1:ompC::gfpBacterial Expression, Synthetic BiologyDisruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28. ColE (from pZ system) + AmpR Add to Cart
30017HL 2300 (=HL 716 + pHL 745)pLtetO-1:RhyB-mCherry, pLlacO-1:sodB::gfpBacterial Expression, Synthetic BiologyDisruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28. ColE (from pZ system) + AmpR Add to Cart
30019HL 2304 (=HL 716 + pHL 269)pLtetO-1:MicC-mCherry, pLlacO-1:ompC::gfpBacterial Expression, Synthetic BiologyDisruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28. ColE (from pZ system) + AmpR Add to Cart
30020HL 2305 (=HL 716 + pHL 765)pLtetO-1:MicC, pLtetO-1:DsrA, pLlacO-1:ompC::gfpBacterial Expression, Synthetic BiologyDisruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28. ColE (from pZ system) + AmpR Add to Cart
30021HL 2307 (=HL 716 + pHL 777)pLtetO-1:MicC, pLtetO-1:RhyB, pLlacO-1:ompC::gfpBacterial Expression, Synthetic BiologyDisruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28. ColE (from pZ system) + AmpR Add to Cart
30022HL 2308 (=HL 716 + pHL 814)pLtetO-1:MicC, pLtetO-1:OxyS, pLlacO-1:ompC::gfpBacterial Expression, Synthetic BiologyDisruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28. ColE (from pZ system) + AmpR Add to Cart
30024HL 2312 (=HL 716 + pHL 787)pLtetO-1:MicC, pLtetO-1:rpoS::mCherry, pLlacO-1:ompC::gfpBacterial Expression, Synthetic BiologyDisruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28. ColE (from pZ system) + AmpR Add to Cart
30025HL 2314 (=HL 716 + pHL 818)pLtetO-1:MicC, pLtetO-1:fhlA::mCherry, pLlacO-1:ompC::gfpBacterial Expression, Synthetic BiologyDisruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28. ColE (from pZ system) + AmpR Add to Cart
30028HL 2625 (=HL 716 + pHL 908)pLlacO-1:RhyB::mCherry, pLtetO-1m9:sodB::gfpBacterial Expression, Synthetic BiologyDisruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28. ColE (from pZ system) + AmpR Add to Cart
30029HL 2627 (=HL 770 + pHL 908)pLlacO-1:RhyB::mCherry, pLtetO-1m9:sodB::gfpBacterial Expression, Synthetic BiologyDisruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28. ColE (from pZ system) + AmpR Add to Cart
30030HL 2729 (=HL 716 + rhyB knockout_KanR)Synthetic BiologyDisruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28. Unknown Add to Cart
30031HL 2817 (=HL 716 + pHL 720)pLtetO-1:mCherry, pLlacO-1:sodB::gfpBacterial Expression, Synthetic BiologyDisruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28. ColE (from pZ system) + AmpR Add to Cart
30032HL 2818 (=HL 865 + pHL 489)pLtetO-1:DsrA, pLlacO-1:hfq, pLtetO-1:rpoS::gfpBacterial Expression, Synthetic BiologyDisruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28. ColE (from pZ system) + AmpR Add to Cart
30033HL 2839 (=HL 2752 + pHL 720)pLtetO-1:mCherry, pLlacO-1:sodB::gfpBacterial Expression, Synthetic BiologyDisruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28. ColE (from pZ system) + AmpR Add to Cart
30034HL 2869 (=HL 2752 + pHL 991)pLtetO-1:RhyB, pLlacO-1:hfq, pLtetO-1m9:sodB::gfpBacterial Expression, Synthetic BiologyDisruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28. ColE (from pZ system) + AmpR Add to Cart
30036HL 3076 (=HL 716 + pHL 1069)pCon:MicC, pLlacO-1:sodB::gfpBacterial Expression, Synthetic BiologyDisruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28. ColE (from pZ system) + AmpR Add to Cart
30047HL 3151 (=HL 716 + pHL 1037)pLtetO-1:DsrA, pCon:MicC, pLlacO-1:rpoS::gfpBacterial Expression, Synthetic BiologyDisruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28. ColE (from pZ system) + AmpR Add to Cart
30048HL 3152 (=HL 716 + pHL 1056)pCon:MicC, pLlacO-1:rpoS::gfpBacterial Expression, Synthetic BiologyDisruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28. ColE (from pZ system) + AmpR Add to Cart
30051HL 3186 (=HL 716 + pHL 282)pLtetO-1:mCherry, pLlacO-1:ompC::gfpBacterial Expression, Synthetic BiologyDisruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28. ColE (from pZ system) + AmpR Add to Cart
30059HL 3326 (=HL 716 + pHL 1008)pLlacO-1:DsrA-mCherry, pLtetO-1:RBS (st7) gfpBacterial Expression, Synthetic BiologyDisruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28. ColE (from pZ system) + AmpR Add to Cart
30060HL 3327 (=HL 716 + pHL 1009)pLlacO-1:MicC-mCherry, pLtetO-1:RBS (st7) gfpBacterial Expression, Synthetic BiologyDisruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28. ColE (from pZ system) + AmpR Add to Cart
30063HL 3330 (=HL 770 + pHL 1009)pLlacO-1:MicC-mCherry, pLtetO-1:RBS (st7) gfpBacterial Expression, Synthetic BiologyDisruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28. ColE (from pZ system) + AmpR Add to Cart
30065HL 3325 (=HL 770 + rhyB knockout_KanR)Synthetic BiologyDisruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28. Unknown Add to Cart
30067HL 3347 (=HL 3338 + pHL 720)pLtetO-1:mCherry, pLlacO-1:sodB::gfpBacterial Expression, Synthetic BiologyDisruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28. ColE (from pZ system) + AmpR Add to Cart
30068HL 3357 (=HL 716 + pHL 1191)pLlacO-1:OxyS-mCherry, pLtetO-1:RBS (st7) gfpBacterial Expression, Synthetic BiologyDisruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28. ColE (from pZ system) + AmpR Add to Cart
30072HL 3375 (=HL 865 + pHL 1008)pLlacO-1:DsrA-mCherry, pLtetO-1:RBS (st7) gfpBacterial Expression, Synthetic BiologyDisruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28. ColE (from pZ system) + AmpR Add to Cart
30074HL 3395 (=HL 862 + pHL 1009)pLlacO-1:MicC-mCherry, pLtetO-1:RBS (st7) gfpBacterial Expression, Synthetic BiologyDisruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28. ColE (from pZ system) + AmpR Add to Cart
30078HL 3448 (=HL 3425 + pHL 1191)pLlacO-1:OxyS-mCherry, pLtetO-1:RBS (st7) gfpBacterial Expression, Synthetic BiologyDisruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28. ColE (from pZ system) + AmpR Add to Cart
30079HL 3449 (=HL 3262 + pHL 1179)pLtetO-1:mCherry, pLlacO-1:fhlA::gfpBacterial Expression, Synthetic BiologyDisruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28. ColE (from pZ system) + AmpR Add to Cart
30080HL 3450 (=HL 3262 + pHL 1191)pLlacO-1:OxyS-mCherry, pLtetO-1:RBS (st7) gfpBacterial Expression, Synthetic BiologyDisruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28. ColE (from pZ system) + AmpR Add to Cart
30081HL 3545 (=HL 770 + pHL 1213)pLlacO-1:hfq, pLtetO-1:RBS (st7) gfpBacterial Expression, Synthetic BiologyDisruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28. ColE (from pZ system) + AmpR Add to Cart
30082HL 3612 (=HL 716 + pHL 841)pLtetO-1:RhyB-mCherry, pLlacO-1:ompC::gfpBacterial Expression, Synthetic BiologyDisruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28. ColE (from pZ system) + AmpR Add to Cart
30083HL 3619 (=HL 716 + pHL 1228)pLtetO-1:DsrA-mCherry, pLlacO-1:ompC::gfpBacterial Expression, Synthetic BiologyDisruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28. ColE (from pZ system) + AmpR Add to Cart
30084HL 3722 (=HL 716 + pHL 404)pLtetO-1:MicC, pLlacO-1:hfq, pLtetO-1:ompC::gfpBacterial Expression, Synthetic BiologyDisruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28. ColE (from pZ system) + AmpR Add to Cart
30085HL 3723 (=HL 716 + pHL 489)pLtetO-1:DsrA, pLlacO-1:hfq, pLtetO-1:rpoS::gfpBacterial Expression, Synthetic BiologyDisruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28. ColE (from pZ system) + AmpR Add to Cart
30086HL 3724 (=HL 716 + pHL 991)pLtetO-1:RhyB, pLlacO-1:hfq, pLtetO-1m9:sodB::gfpBacterial Expression, Synthetic BiologyDisruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28. ColE (from pZ system) + AmpR Add to Cart
30088HL 3858 (=HL 716 + pHL 1304)pLtetO-1:rpoS::mCherry, pLlacO-1:ompC::gfpBacterial Expression, Synthetic BiologyDisruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28. ColE (from pZ system) + AmpR Add to Cart
30089HL 3859 (=HL 716 + pHL 1305)pLtetO-1:fhlA::mCherry, pLlacO-1:ompC::gfpBacterial Expression, Synthetic BiologyDisruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28. ColE (from pZ system) + AmpR Add to Cart
30093HL 3904 (=HL 770 + pHL 991)pLtetO-1:RhyB, pLlacO-1:hfq, pLtetO-1m9:sodB::gfpBacterial Expression, Synthetic BiologyDisruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28. ColE (from pZ system) + AmpR Add to Cart
30094HL 4120 (=HL 716 + pHL 1370)pLtetO-1:DsrA, pCon:T7(st3) (900bps), pLlacO-1:rpoS::gfpBacterial Expression, Synthetic BiologyDisruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28. ColE (from pZ system) + AmpR Add to Cart
30096HL 4175 (=HL 716 + pHL 1391)pLlacO-1:rpoS::gfpBacterial Expression, Synthetic BiologyDisruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28. ColE (from pZ system) + AmpR Add to Cart
30097HL 4178 (=HL 862 + pHL 1360)pLlacO-1:hfq, pLtetO-1:ompC::gfpBacterial Expression, Synthetic BiologyDisruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28. ColE (from pZ system) + AmpR Add to Cart
30098HL 4179 (=HL 865 + pHL 1381)pLlacO-1:hfq, pLtetO-1:rpoS::gfpBacterial Expression, Synthetic BiologyDisruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28. ColE (from pZ system) + AmpR Add to Cart
30099HL 4180 (=HL 2752 + pHL 1358)pLlacO-1:hfq, pLtetO-1m9:sodB::gfpBacterial Expression, Synthetic BiologyDisruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28. ColE (from pZ system) + AmpR Add to Cart
30100HL 4202 (=HL 716 + pHL 1389)pLtetO-1:DsrA, pLlacO-1:rpoS::gfpBacterial Expression, Synthetic BiologyDisruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28. ColE (from pZ system) + AmpR Add to Cart
30104HL 4212 (=HL 716 + pHL 1414)pCon:fhlA::T7noRBS(900bps), pLlacO-1:rpoS::gfpBacterial Expression, Synthetic BiologyDisruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28. ColE (from pZ system) + AmpR Add to Cart
30105HL 4218 (=HL 716 + pHL 1411)pLtetO-1:DsrA, pCon:fhlA:T7noRBS(900bps), pLlacO-1:rpoS::gfp (Other)Bacterial Expression, Synthetic BiologyDisruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28. ColE (from pZ system) + AmpR Add to Cart
37563pHL600CsrB, CsrABacterial Expression, Synthetic BiologyRapid and robust signaling in the CsrA cascade via RNA-protein interactions and feedback regulation. Proc Natl Acad Sci U S A. 2013 Aug 6;110(32):13120-5. doi: 10.1073/pnas.1308476110. Epub 2013 Jul 22. pZ with p15a origin csrB b4408, ECK2787, JWR0062 csrA b2696, ECK2691, JW2666, zfiA Add to Cart
37564pHL601CsrC, CsrABacterial Expression, Synthetic BiologyRapid and robust signaling in the CsrA cascade via RNA-protein interactions and feedback regulation. Proc Natl Acad Sci U S A. 2013 Aug 6;110(32):13120-5. doi: 10.1073/pnas.1308476110. Epub 2013 Jul 22. pZ with p15a origin csrC b4457, ECK3858, IS198, JWR0214, psrA21, ryiB, sraK, tpk2 csrA b2696, ECK2691, JW2666, zfiA Add to Cart
37566pHL1355GFP, TetR, CsrABacterial Expression, Synthetic BiologyRapid and robust signaling in the CsrA cascade via RNA-protein interactions and feedback regulation. Proc Natl Acad Sci U S A. 2013 Aug 6;110(32):13120-5. doi: 10.1073/pnas.1308476110. Epub 2013 Jul 22. pZ with ColE origin
  • Tag / Fusion Protein
    • glgC leader region (N terminal on backbone)
  • Add to Cart
    37569pHL1530GFP, TetR, CsrABacterial Expression, Synthetic BiologyRapid and robust signaling in the CsrA cascade via RNA-protein interactions and feedback regulation. Proc Natl Acad Sci U S A. 2013 Aug 6;110(32):13120-5. doi: 10.1073/pnas.1308476110. Epub 2013 Jul 22. pZ with ColE origin
  • Tag / Fusion Protein
    • glgC leader region (N terminal on backbone)
  • Add to Cart
    37570pHL1559CsrBBacterial Expression, Synthetic BiologyRapid and robust signaling in the CsrA cascade via RNA-protein interactions and feedback regulation. Proc Natl Acad Sci U S A. 2013 Aug 6;110(32):13120-5. doi: 10.1073/pnas.1308476110. Epub 2013 Jul 22. pZ with p15a origin csrB b4408, ECK2787, JWR0062 Add to Cart
    37571pHL1561CsrABacterial Expression, Synthetic BiologyRapid and robust signaling in the CsrA cascade via RNA-protein interactions and feedback regulation. Proc Natl Acad Sci U S A. 2013 Aug 6;110(32):13120-5. doi: 10.1073/pnas.1308476110. Epub 2013 Jul 22. pZ with p15a origin csrA b2696, ECK2691, JW2666, zfiA Add to Cart
    37633pHL1668GFP, TetR, CsrABacterial Expression, Synthetic BiologyRapid and robust signaling in the CsrA cascade via RNA-protein interactions and feedback regulation. Proc Natl Acad Sci U S A. 2013 Aug 6;110(32):13120-5. doi: 10.1073/pnas.1308476110. Epub 2013 Jul 22. pZ with ColE origin csrA b2696, ECK2691, JW2666, zfiA Add to Cart
    37634pHL1670GFP, TetR, CsrABacterial Expression, Synthetic BiologyRapid and robust signaling in the CsrA cascade via RNA-protein interactions and feedback regulation. Proc Natl Acad Sci U S A. 2013 Aug 6;110(32):13120-5. doi: 10.1073/pnas.1308476110. Epub 2013 Jul 22. pZ with ColE origin
  • Tags / Fusion Proteins
    • ASV degradation tag (C terminal on backbone)
    • glgC leader region (N terminal on backbone)
  • csrA b2696, ECK2691, JW2666, zfiA
    Add to Cart
    37635pHL661GFP, mCherryBacterial Expression, Synthetic BiologyRapid and robust signaling in the CsrA cascade via RNA-protein interactions and feedback regulation. Proc Natl Acad Sci U S A. 2013 Aug 6;110(32):13120-5. doi: 10.1073/pnas.1308476110. Epub 2013 Jul 22. pZ with p15a origin Add to Cart
    37636pHL662mCherry, GFPBacterial Expression, Synthetic BiologyRapid and robust signaling in the CsrA cascade via RNA-protein interactions and feedback regulation. Proc Natl Acad Sci U S A. 2013 Aug 6;110(32):13120-5. doi: 10.1073/pnas.1308476110. Epub 2013 Jul 22. pZ with p15a origin Add to Cart
    37637pHL1318TetR, CsrABacterial Expression, Synthetic BiologyRapid and robust signaling in the CsrA cascade via RNA-protein interactions and feedback regulation. Proc Natl Acad Sci U S A. 2013 Aug 6;110(32):13120-5. doi: 10.1073/pnas.1308476110. Epub 2013 Jul 22. pZ with ColE origin Add to Cart
    37638pHL1324GFP, TetR, CsrABacterial Expression, Synthetic BiologyRapid and robust signaling in the CsrA cascade via RNA-protein interactions and feedback regulation. Proc Natl Acad Sci U S A. 2013 Aug 6;110(32):13120-5. doi: 10.1073/pnas.1308476110. Epub 2013 Jul 22. pZ with ColE origin Add to Cart
    37754pHL 233ptet_ompC_gfp, pLac_st7 TetR_mCherrySynthetic Biology ; E. coliDirect comparison of small RNA and transcription factor signaling. Nucleic Acids Res. 2012 May 22. ColE (from pZ system) + AmpR Add to Cart
    37757pHL 279ptet_ompC_gfp, pLac_st3 TetR_mCherrySynthetic Biology ; E. coliDirect comparison of small RNA and transcription factor signaling. Nucleic Acids Res. 2012 May 22. ColE (from pZ system) + AmpR
  • Tags / Fusion Proteins
    • gfp (C terminal on insert)
    • mCherry (C terminal on insert)
  • Add to Cart
    37758pHL 363pLar_rpoS_gfp, pLtet_dsrA_pLac_araCSynthetic Biology ; E. coliDirect comparison of small RNA and transcription factor signaling. Nucleic Acids Res. 2012 May 22. ColE (from pZ system) + AmpR
  • Tags / Fusion Proteins
    • gfp (C terminal on insert)
    • none
  • Add to Cart
    37759pHL 1569ptet_ompC_gfpAaV, pLac_micC_mCherrySynthetic Biology ; E. coliDirect comparison of small RNA and transcription factor signaling. Nucleic Acids Res. 2012 May 22. ColE (from pZ system) + AmpR
  • Tags / Fusion Proteins
    • gfp (C terminal on insert)
    • mCherry (C terminal on insert)
  • Add to Cart
    37760pHL 1571ptet_ompC_gfpAaV, pLac_st3 TetR_mCherrySynthetic Biology ; E. coliDirect comparison of small RNA and transcription factor signaling. Nucleic Acids Res. 2012 May 22. ColE (from pZ system) + AmpR
  • Tags / Fusion Proteins
    • gfp (C terminal on insert)
    • mCherry (C terminal on insert)
  • Add to Cart
    37976HL13See commentsSynthetic BiologyA multistep epigenetic switch enables the stable inheritance of DNA methylation states. Nat Genet. 2007 Feb . 39(2):269-75. N/A Add to Cart
    37977HL23See comments, See commentsSynthetic BiologyA multistep epigenetic switch enables the stable inheritance of DNA methylation states. Nat Genet. 2007 Feb . 39(2):269-75. N/A Add to Cart
    37978HL24See commentsSynthetic BiologyA multistep epigenetic switch enables the stable inheritance of DNA methylation states. Nat Genet. 2007 Feb . 39(2):269-75. N/A Add to Cart
    37979HL168See commentsSynthetic BiologyA multistep epigenetic switch enables the stable inheritance of DNA methylation states. Nat Genet. 2007 Feb . 39(2):269-75. N/A Add to Cart
    37980HL3796See commentsSynthetic Biology Han Lim Lab Strains (unpublished) N/A Add to Cart
    37981HL4142See commentsSynthetic BiologyRapid and robust signaling in the CsrA cascade via RNA-protein interactions and feedback regulation. Proc Natl Acad Sci U S A. 2013 Aug 6;110(32):13120-5. doi: 10.1073/pnas.1308476110. Epub 2013 Jul 22. N/A Add to Cart
    37982HL5086See commentsSynthetic BiologyMinimal effect of gene clustering on expression in Escherichia coli. Genetics. 2013 Feb;193(2):453-65. doi: 10.1534/genetics.112.147199. Epub 2012 Dec 5. N/A Add to Cart
    37983HL5113See commentsSynthetic BiologyMinimal effect of gene clustering on expression in Escherichia coli. Genetics. 2013 Feb;193(2):453-65. doi: 10.1534/genetics.112.147199. Epub 2012 Dec 5. N/A Add to Cart
    37984HL3515See commentsSynthetic BiologyFundamental relationship between operon organization and gene expression. Proc Natl Acad Sci U S A. 2011 Jun 28;108(26):10626-31. doi: 10.1073/pnas.1105692108. Epub 2011 Jun 13. N/A Add to Cart
    37985HL3953See commentsSynthetic BiologyMinimal effect of gene clustering on expression in Escherichia coli. Genetics. 2013 Feb;193(2):453-65. doi: 10.1534/genetics.112.147199. Epub 2012 Dec 5. N/A Add to Cart
    37986HL3546See commentsSynthetic BiologyFundamental relationship between operon organization and gene expression. Proc Natl Acad Sci U S A. 2011 Jun 28;108(26):10626-31. doi: 10.1073/pnas.1105692108. Epub 2011 Jun 13. N/A Add to Cart
    37987HL3573See commentsSynthetic BiologyMinimal effect of gene clustering on expression in Escherichia coli. Genetics. 2013 Feb;193(2):453-65. doi: 10.1534/genetics.112.147199. Epub 2012 Dec 5. N/A Add to Cart
    37988HL3578See commentsSynthetic BiologyMinimal effect of gene clustering on expression in Escherichia coli. Genetics. 2013 Feb;193(2):453-65. doi: 10.1534/genetics.112.147199. Epub 2012 Dec 5. N/A Add to Cart
    52931HL 713none (Other)Synthetic BiologyDisruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28. MG 1655 + lacIq_KanR inserted into the chromosome at the intS site none Add to Cart
    52932HL 751none (Other)Synthetic BiologyDisruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28. MG 1655 + lacIq inserted into the chromosome at the intS site + Δhfq_KanR none Add to Cart
    52933HL 852none (Other)Synthetic BiologyDisruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28. MG 1655 + lacIq inserted into the chromosome at the intS site + ΔdsrA_KanR none Add to Cart
    52934HL 1120none (Other)Synthetic BiologyDisruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28. MG 1655 + lacIq inserted into the chromosome at the intS site + ΔmicC + Δhfq_KanR none Add to Cart
    52935HL 1179none (Other)Synthetic BiologyDisruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28. MG 1655 + lacIq inserted into the chromosome at the intS site + ΔdsrA + Δhfq_KanR none Add to Cart
    52936HL 3221none (Other)Synthetic BiologyDisruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28. MG 1655 + lacIq inserted into the chromosome at the intS site + ΔoxyS_KanR none Add to Cart
    52937HL 3387none (Other)Synthetic BiologyDisruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28. MG 1655 + lacIq inserted into the chromosome at the intS site + Δhfq + ΔoxyS_KanR none Add to Cart
    52938HL 1216none (Other)Synthetic BiologyDirect comparison of small RNA and transcription factor signaling. Nucleic Acids Res. 2012 May 22. MG 1655 + lacIq inserted into the chromosome at the intS site + ΔmicC + ΔaraC_KanR none Add to Cart
    52939HL 1254none (Other)Synthetic BiologyDirect comparison of small RNA and transcription factor signaling. Nucleic Acids Res. 2012 May 22. MG 1655 + lacIq inserted into the chromosome at the intS site + ΔdsrA + ΔaraC_KanR none Add to Cart
    52940HL 932none (Other)Synthetic BiologyModulating the frequency and bias of stochastic switching to control phenotypic variation. Nat Commun. 2014 Aug 4;5:4574. doi: 10.1038/ncomms5574. MG1655 + ΔlacI none Add to Cart
    52941HL 1255none (Other)Synthetic BiologyModulating the frequency and bias of stochastic switching to control phenotypic variation. Nat Commun. 2014 Aug 4;5:4574. doi: 10.1038/ncomms5574. MG1655 + fimS (off orientation)::gfp::Asp terminator::CamR at fimA none Add to Cart
    52942HL 1285none (Other)Synthetic BiologyModulating the frequency and bias of stochastic switching to control phenotypic variation. Nat Commun. 2014 Aug 4;5:4574. doi: 10.1038/ncomms5574. MG1655 + fimS (off orientation)::gfp::Asp terminator at fimA none Add to Cart
    52943HL 4195none (Other)Synthetic BiologyModulating the frequency and bias of stochastic switching to control phenotypic variation. Nat Commun. 2014 Aug 4;5:4574. doi: 10.1038/ncomms5574. MG1655 + fimS (off orientation)::gfp::Asp terminator at fimA + ΔlacI + KanR replaces fimB and fimE(partial) none Add to Cart
    52944HL 4233none (Other)Synthetic BiologyModulating the frequency and bias of stochastic switching to control phenotypic variation. Nat Commun. 2014 Aug 4;5:4574. doi: 10.1038/ncomms5574. ΔfimB + ΔfimE + ΔfimA none Add to Cart
    52946HL 6139none (Other)Synthetic BiologyModulating the frequency and bias of stochastic switching to control phenotypic variation. Nat Commun. 2014 Aug 4;5:4574. doi: 10.1038/ncomms5574. MG1655 + fimS (off orientation)::gfp::Asp terminator at fimA + ΔlacI + KanR replaces fimB and fimE(stop codon) none Add to Cart
    52947HL 6166none (Other)Synthetic BiologyModulating the frequency and bias of stochastic switching to control phenotypic variation. Nat Commun. 2014 Aug 4;5:4574. doi: 10.1038/ncomms5574. MG1655 + fimS (off orientation)::gfp::Asp terminator at fimA + ΔlacI + KanR replaces fimB and fimE(IRL) none Add to Cart
    52949HL 6279none (Other)Synthetic BiologyModulating the frequency and bias of stochastic switching to control phenotypic variation. Nat Commun. 2014 Aug 4;5:4574. doi: 10.1038/ncomms5574. MG1655 + fimS (off orientation)::gfpAAV tag::Asp terminator::CamR at fimA + ΔlacI + KanR replaces fimB and fimE(partial) none Add to Cart
    52950HL3721none (Other)Synthetic BiologyRapid and robust signaling in the CsrA cascade via RNA-protein interactions and feedback regulation. Proc Natl Acad Sci U S A. 2013 Aug 6;110(32):13120-5. doi: 10.1073/pnas.1308476110. Epub 2013 Jul 22. HL716 + ΔcsrB + ΔcsrC + ΔglgCAP + ΔpgaABCD none Add to Cart
    52951HL3796none (Other)Synthetic Biology Lim Lab unpublished plasmids (csrA crosstalk) (unpublished) HL716 + ΔcsrA + ΔcsrB + ΔcsrC + ΔglgCAP + ΔpgaABCD none Add to Cart
    52952HL4018none (Other)Synthetic Biology Lim Lab unpublished plasmids (csrA crosstalk) (unpublished) HL716 + ΔcsrB + ΔcsrC + ΔcsrD + ΔglgCAP + ΔpgaABCD none Add to Cart
    52989pHL 100OxyS sRNA (Other), fhlA (Other)Bacterial Expression, Synthetic BiologyDisruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28. pLlac0-1:OxyS-mCherry, pLtetO-1:fhlA::gfp unknown
  • Tag / Fusion Protein
    • RBS-mCherry (C terminal on insert)
  • Add to Cart
    52990pHL 762MicC sRNA (Other), sodB (Other), ompC (Other)Bacterial Expression, Synthetic BiologyDisruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28. pLtetO-1:MicC, pLtetO-1:sodB::mCherry, pLlacO-1:ompC::gfp unknown Add to Cart
    52991pHL 802OxyS sRNA (Other), ompC (Other)Bacterial Expression, Synthetic BiologyDisruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28. pLtetO-1:OxyS-mCherry, pLlacO-1:ompC::gfp unknown
  • Tag / Fusion Protein
    • RBS-mCherry (C terminal on insert)
  • Add to Cart
    52992pHL 990DsrA sRNA (Other), RhyB sRNA (competitor) (Other), rpoS (Other)Bacterial Expression, Synthetic BiologyDisruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28. pLtetO-1:DsrA, pCon:RhyB, pLlacO-1:rpoS::gfp unknown Add to Cart
    52993pHL 1011RhyB sRNA (Other), RBS (st7) (Synthetic)Bacterial Expression, Synthetic BiologyDisruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28. pLlac0-1:RhyB-mCherry, pLtetO-1:RBS (st7) gfp unknown
  • Tag / Fusion Protein
    • RBS-mCherry (C terminal on insert)
  • Add to Cart
    52994pHL 1019RhyB sRNA (Other), MicC sRNA (Competitor) (Other), sodB (Other)Bacterial Expression, Synthetic BiologyDisruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28. pLtetO-1:RhyB, pCon:MicC, pLlacO-1:sodB::gfp unknown Add to Cart
    52995pHL 1020RhyB sRNA (Other), DsrA sRNA (competitor) (Other), sodB (Other)Bacterial Expression, Synthetic BiologyDisruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28. pLtetO-1:RhyB, pCon:DsrA, pLlacO-1:sodB::gfp unknown Add to Cart
    52996pHL 1022RhyB sRNA (Other), OxyS sRNA (Other), sodB (Other)Bacterial Expression, Synthetic BiologyDisruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28. pLtetO-1:RhyB, pCon:OxyS, pLlacO-1:sodB::gfp unknown Add to Cart
    52997pHL 1032RhyB sRNA (Other), ompC (Other), sodB (Other)Bacterial Expression, Synthetic BiologyDisruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28. pLtetO-1:RhyB, pCon:ompC::mCherry, pLlacO-1:sodB::gfp unknown Add to Cart
    52998pHL 1033RhyB sRNA (Other), rpoS (Other), sodB (Other)Bacterial Expression, Synthetic BiologyDisruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28. pLtetO-1:RhyB, pCon:rpoS::mCherry, pLlacO-1:sodB::gfp unknown Add to Cart
    52999pHL 1035RhyB sRNA (Other), fhlA (Other), sodB (Other)Bacterial Expression, Synthetic BiologyDisruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28. pLtetO-1:RhyB, pCon:fhlA::mCherry, pLlacO-1:sodB::gfp unknown Add to Cart
    53000pHL 1039DsrA sRNA (Other), OxyS sRNA (competitor) (Other), rpoS (Other)Bacterial Expression, Synthetic BiologyDisruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28. pLtetO-1:DsrA, pCon:OxyS, pLlacO-1:rpoS::gfp unknown Add to Cart
    53001pHL 1057RhyB sRNA (Other), rpoS (Other)Bacterial Expression, Synthetic BiologyDisruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28. pCon:RhyB, pLlacO-1:rpoS::gfp unknown Add to Cart
    53002pHL 1070DsrA sRNA (Other), sodB (Other)Bacterial Expression, Synthetic BiologyDisruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28. pCon:DsrA, pLlacO-1:sodB::gfp unknown Add to Cart
    53003pHL 1073mCherry (Other), GFP (Other)Bacterial Expression, Synthetic BiologyDisruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28. pCon:ompC::mCherry, pLlacO-1:sodB::gfp unknown
  • Tag / Fusion Protein
    • ompC (target mRNA) (N terminal on insert)
  • Add to Cart
    53004pHL 1075fhlA (Other), sodB (Other)Bacterial Expression, Synthetic BiologyDisruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28. pCon:fhlA::mCherry, pLlacO-1:sodB::gfp unknown
  • Tag / Fusion Protein
    • mCherry (C terminal on insert)
  • Add to Cart
    53006pHL 1148OxyS sRNA (Other), sodB (Other)Bacterial Expression, Synthetic BiologyDisruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28. pCon:OxyS, pLlacO-1:sodB::gfp unknown Add to Cart
    53007pHL 1158RhyB sRNA (Other), DsrA sRNA (Other), ompC (Other), sodB (Other)Bacterial Expression, Synthetic BiologyDisruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28. pLtetO-1:RhyB, pCon:DsrA, pCon:ompC::mCherry, pLlacO-1:sodB::gfp unknown Add to Cart
    53008pHL 1303sodB (Other), ompC (Other)Bacterial Expression, Synthetic BiologyDisruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28. pLtetO-1:sodB::mCherry, pLlacO-1:ompC::gfp unknown
  • Tag / Fusion Protein
    • mCherry (C terminal on insert)
  • Add to Cart
    53009pHL 1394RhyB sRNA (Other), T7(st3) (133bps) (Other), sodB (Other)Bacterial Expression, Synthetic BiologyDisruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28. pLtetO-1:RhyB, pCon:T7(st3) (133bps)::mCherry, pLlacO-1:sodB::gfp unknown Add to Cart
    53010pHL 1405RhyB sRNA (Other), DsrA sRNA (Other), hfq (Other), sodB (Other)Bacterial Expression, Synthetic BiologyDisruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28. pLtetO-1:RhyB, pCon:DsrA, pLtetO-1:hfq, pLlacO-1:sodB::gfp unknown Add to Cart
    53011pHL 1410DsrA sRNA (Other), ompC (Other), rpoS (Other)Bacterial Expression, Synthetic BiologyDisruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28. pLtetO-1:DsrA, pCon:ompC::T7noRBS(900bps), pLlacO-1:rpoS::gfp unknown Add to Cart
    53012pHL 342mCherry (Synthetic), araC (Other), ompC (Other)Bacterial Expression, Synthetic BiologyDirect comparison of small RNA and transcription factor signaling. Nucleic Acids Res. 2012 May 22. PLtetO-1:mCherry, PLlacO-1:araC, Plar:ompC::gfp unknown Add to Cart
    53013pHL 1023mCherry (Synthetic), gfp (st7) (Synthetic)Bacterial Expression, Synthetic BiologyDirect comparison of small RNA and transcription factor signaling. Nucleic Acids Res. 2012 May 22. PLtetO-1:mCherry, PLlacO-1:gfp (st7) unknown Add to Cart
    53014pHL 246GFP (Other)Bacterial Expression, Synthetic BiologyModulating the frequency and bias of stochastic switching to control phenotypic variation. Nat Commun. 2014 Aug 4;5:4574. doi: 10.1038/ncomms5574. AmpR + fimS (off orientation)::gfp::Asp terminator + CamR + ColE1 unknown
  • Tag / Fusion Protein
    • fimS (off orientation) (N terminal on insert)
  • Add to Cart
    53016pHL 651LacIq (Other), tetR (Other)Bacterial Expression, Synthetic BiologyModulating the frequency and bias of stochastic switching to control phenotypic variation. Nat Commun. 2014 Aug 4;5:4574. doi: 10.1038/ncomms5574. LacIq + PCon::RBS (st3) tetR::T1 terminator + p15a + CamR. Backbone from pZA32MCS1 (Lutz and Bujard, 1997). pZA32MCS1 (Lutz and Bujard, 1997). Add to Cart
    53017pHL 1277 GFP (Other), mCherry (Synthetic)Bacterial Expression, Synthetic BiologyModulating the frequency and bias of stochastic switching to control phenotypic variation. Nat Commun. 2014 Aug 4;5:4574. doi: 10.1038/ncomms5574. AmpR + PLtetO-1::RBS (st7) gfp::T1 terminator + PLlacO-1::RBS (st7) mCherry::Asp terminator + ColE1 unknown Add to Cart
    53018pHL 1278mCherry (Synthetic), GFP (Other)Bacterial Expression, Synthetic BiologyModulating the frequency and bias of stochastic switching to control phenotypic variation. Nat Commun. 2014 Aug 4;5:4574. doi: 10.1038/ncomms5574. AmpR + PLtetO-1::RBS (st7) mCherry::T1 terminator + PLlacO-1::RBS (st7) gfp::Asp terminator + ColE1 unknown
  • Tag / Fusion Protein
    • RBS(st7) (N terminal on insert)
  • Add to Cart
    53019pHL 1454fimB (Other)Bacterial Expression, Synthetic BiologyModulating the frequency and bias of stochastic switching to control phenotypic variation. Nat Commun. 2014 Aug 4;5:4574. doi: 10.1038/ncomms5574. AmpR + PLlacO-1::RBS (st7) fimB::Asp terminator + ColE1 unknown Add to Cart
    53020pHL 1903GFP (Other)Bacterial Expression, Synthetic BiologyModulating the frequency and bias of stochastic switching to control phenotypic variation. Nat Commun. 2014 Aug 4;5:4574. doi: 10.1038/ncomms5574. AmpR + fimS::gfpAAV::Asp terminator + CamR + ColE1 unknown Add to Cart
    53021pHL 1904GFP (Other)Bacterial Expression, Synthetic Biology Lim Lab plasmids (unpublished) AmpR + fimS::gfpLVA::Asp terminator + CamR + ColE1 unknown Add to Cart
    53022pHL 1915GFP (Other)Bacterial Expression, Synthetic Biology Lim Lab plasmids (unpublished) AmpR + PLlacO1fims::gfp::Asp terminator + CamR + ColE1 (PLlacO1fimS is a fused PLlacO-1 and fimS promoter) unknown Add to Cart
    53023pHL 1916fimB (Other)Bacterial Expression, Synthetic BiologyModulating the frequency and bias of stochastic switching to control phenotypic variation. Nat Commun. 2014 Aug 4;5:4574. doi: 10.1038/ncomms5574. AmpR + PLtetO-1::RBS (st7) fimB::Asp terminator + ColE1 unknown Add to Cart
    53024pHL 1917mCherry (Synthetic), fimB (Other)Bacterial Expression, Synthetic BiologyModulating the frequency and bias of stochastic switching to control phenotypic variation. Nat Commun. 2014 Aug 4;5:4574. doi: 10.1038/ncomms5574. AmpR + PCon:RBS (st7)::mCherry::T1 terminator + PLlacO-1::RBS (st7) fimB::Asp terminator + ColE1 unknown
  • Tag / Fusion Protein
    • RBS(st7) (N terminal on insert)
  • fimB b4312, ECK4303, JW4275
    Add to Cart
    53025pHL 1977fimB (Other)Bacterial Expression, Synthetic Biology Lim Lab plasmids (unpublished) AmpR + PLtetO-1::RBS (st3) fimB::Asp terminator + ColE1 unknown Add to Cart
    53026pHL1506csrA (Other)Bacterial Expression, Synthetic BiologyRapid and robust signaling in the CsrA cascade via RNA-protein interactions and feedback regulation. Proc Natl Acad Sci U S A. 2013 Aug 6;110(32):13120-5. doi: 10.1073/pnas.1308476110. Epub 2013 Jul 22. PLlacO-1:RBS(st7):csrA unknown Add to Cart
    53027pHL1529GFP (Other), tetR, csrA (Other)Bacterial Expression, Synthetic BiologyRapid and robust signaling in the CsrA cascade via RNA-protein interactions and feedback regulation. Proc Natl Acad Sci U S A. 2013 Aug 6;110(32):13120-5. doi: 10.1073/pnas.1308476110. Epub 2013 Jul 22. PLlacO-1:glgC::gfp, PconNoHind:RBS(st3):tetR, PconNoHind:RBS(st2):csrA unknown
  • Tag / Fusion Protein
    • glgC leader contains -61 to +8 relative to the start codon (N terminal on insert)
  • Add to Cart
    53028pHL1575csrB (Other), csrD (Other)Bacterial Expression, Synthetic BiologyRapid and robust signaling in the CsrA cascade via RNA-protein interactions and feedback regulation. Proc Natl Acad Sci U S A. 2013 Aug 6;110(32):13120-5. doi: 10.1073/pnas.1308476110. Epub 2013 Jul 22. PconNoHind:csrB, PLtetO-1:RBS(st3):csrD unknown Add to Cart
    53029pHL1590csrA (Other)Bacterial Expression, Synthetic Biology Lim Lab plasmids (unpublished) PLtetO-1:RBS(st3):csrA unknown Add to Cart
    53030pHL1722mCherry (Other), csrA (Other)Bacterial Expression, Synthetic Biology Lim Lab plasmids (unpublished) PLtetO-1:glgClead::mCherry, PLlacO-1:RBS(st7):csrA unknown
  • Tag / Fusion Protein
    • glgC leader (N terminal on insert)
  • Add to Cart
    53031pHL1724mCherry (Other), csrA (Other)Bacterial Expression, Synthetic Biology Lim Lab plasmids (unpublished) PLtetO-1:pgaAleadFull::mCherry, PLlacO-1:RBS(st7):csrA unknown
  • Tag / Fusion Protein
    • pgaAleadFull (N terminal on insert)
  • Add to Cart
    53032pHL1726mCherry (Other), csrA (Other)Bacterial Expression, Synthetic Biology Lim Lab plasmids (unpublished) PLtetO-1:hfqlead::mCherry, PLlacO-1:RBS(st7):csrA unknown
  • Tag / Fusion Protein
    • hfqlead (N terminal on insert)
  • Add to Cart
    53033pHL1745mCherry (Other), csrA (Other)Bacterial Expression, Synthetic Biology Lim Lab plasmids (unpublished) PLtetO-1:cstAlead::mCherry, PLlacO-1:RBS(st7):csrA unknown
  • Tag / Fusion Protein
    • cstAlead (N terminal on insert)
  • Add to Cart
    53034pHL1746mCherry (Other), csrA (Other)Bacterial Expression, Synthetic Biology Lim Lab plasmids (unpublished) PLtetO-1:ycdTlead::mCherry, PLlacO-1:RBS(st7):csrA unknown
  • Tag / Fusion Protein
    • ycdTlead (N terminal on insert)
  • Add to Cart
    53035pHL1747mCherry (Other), csrA (Other)Bacterial Expression, Synthetic Biology Lim Lab plasmids (unpublished) PLtetO-1:ydeHlead::mCherry, PLlacO-1:RBS(st7):csrA unknown Add to Cart
    53036pHL1756GFP (Other), tetR (Other)Bacterial Expression, Synthetic BiologyRapid and robust signaling in the CsrA cascade via RNA-protein interactions and feedback regulation. Proc Natl Acad Sci U S A. 2013 Aug 6;110(32):13120-5. doi: 10.1073/pnas.1308476110. Epub 2013 Jul 22. PconNoHindM12:glgC::gfp, PconNoHind:RBS(st3):tetR unknown
  • Tag / Fusion Protein
    • glgC leader (N terminal on insert)
  • Add to Cart
    53037pHL1757GFP (Other), tetR (Other)Bacterial Expression, Synthetic BiologyRapid and robust signaling in the CsrA cascade via RNA-protein interactions and feedback regulation. Proc Natl Acad Sci U S A. 2013 Aug 6;110(32):13120-5. doi: 10.1073/pnas.1308476110. Epub 2013 Jul 22. PconNoHindM2:glgC::gfp, PconNoHind:RBS(st3):tetR unknown Add to Cart
    53038pHL1790mCherry (Other), csrB (Other)Bacterial Expression, Synthetic Biology Lim Lab plasmids (unpublished) PLtetO-1:glgClead::mCherry, PLlacO-1:csrB unknown Add to Cart
    53039pHL1801GFP (Other)Bacterial Expression, Synthetic BiologyRapid and robust signaling in the CsrA cascade via RNA-protein interactions and feedback regulation. Proc Natl Acad Sci U S A. 2013 Aug 6;110(32):13120-5. doi: 10.1073/pnas.1308476110. Epub 2013 Jul 22. PLlacO-1:RBS(st7):gfp unknown
  • Tag / Fusion Protein
    • RBS(st7) (N terminal on insert)
  • Add to Cart
    53040pHL1853GFP (Other), tetR (Other), csrA (Other)Bacterial Expression, Synthetic BiologyRapid and robust signaling in the CsrA cascade via RNA-protein interactions and feedback regulation. Proc Natl Acad Sci U S A. 2013 Aug 6;110(32):13120-5. doi: 10.1073/pnas.1308476110. Epub 2013 Jul 22. PLlacO-1:glgC::gfp::LVA, PconNoHind:RBS(st3):tetR, PconNoHind:RBS(st2):csrA unknown
  • Tag / Fusion Protein
    • glgC leader (N terminal on insert)
  • Add to Cart
    53041pHL1855GFP (Other), tetR (Other), csrA (Other)Bacterial Expression, Synthetic BiologyRapid and robust signaling in the CsrA cascade via RNA-protein interactions and feedback regulation. Proc Natl Acad Sci U S A. 2013 Aug 6;110(32):13120-5. doi: 10.1073/pnas.1308476110. Epub 2013 Jul 22. PLlacO-1:glgC::gfp::LVA, PconNoHind:RBS(st3):tetR, PconNoHind:RBS(st3):csrA unknown Add to Cart
    53042pHL1914GFP (Other), tetR (Other)Bacterial Expression, Synthetic Biology Lim Lab plasmids (unpublished) PconNoHindM2:hfqlead::gfp, PconNoHind:RBS(st3):tetR unknown
  • Tag / Fusion Protein
    • hfqlead (N terminal on insert)
  • Add to Cart
    53043pHL1928mCherry (Other), csrA (Other)Bacterial Expression, Synthetic Biology Lim Lab plasmids (unpublished) PLtetO-1:Agn43RBS:mCherry, PLlacO-1:RBS(st7):csrA unknown
  • Tag / Fusion Protein
    • Agn43RBS (C terminal on insert)
  • Add to Cart
    53044pHL1940cstA (Other), csrA (Other)Bacterial Expression, Synthetic Biology Lim Lab plasmids (unpublished) PLtetO-1:cstA‡, PLlacO-1:RBS(st7):csrA unknown Add to Cart
    53045pHL1947GFP (Other), tetR (Other), csrA (Other)Bacterial Expression, Synthetic Biology Lim Lab plasmids (unpublished) PconNoHindM12:glgC::gfp, PconNoHind:RBS(st3):tetR, PconNoHindM8:RBS(st3):csrA unknown
  • Tag / Fusion Protein
    • glgC leader (N terminal on insert)
  • Add to Cart
    53046pHL1948GFP (Other), tetR (Other), csrA (Other)Bacterial Expression, Synthetic Biology Lim Lab plasmids (unpublished) PconNoHindM12:glgC::gfp, PconNoHind:RBS(st3):tetR, PconNoHindM10:RBS(st3):csrA unknown Add to Cart
    60368HL 716See commentsSynthetic BiologyDisruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28. Lim Lab Strain N/A Add to Cart
    60369HL 770See commentsSynthetic BiologyDisruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28. Lim Lab Strain N/A Add to Cart
    60370HL 862See commentsSynthetic BiologyDisruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28. Lim Lab Strain N/A Add to Cart
    60371HL 865See commentsSynthetic BiologyDisruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28. Lim Lab Strain N/A Add to Cart
    60372HL 1128See commentsSynthetic BiologyDisruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28. Lim Lab Strain N/A Add to Cart
    60373HL 1188See commentsSynthetic BiologyDisruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28. Lim Lab Strain N/A Add to Cart
    60374HL 2752See commentsSynthetic BiologyDisruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28. Lim Lab Strain N/A Add to Cart
    60376HL 3338See commentsSynthetic BiologyDisruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28. Lim Lab Strain N/A Add to Cart
    61160HL 1745See commentsSynthetic BiologyRegulatory consequences of gene translocation in bacteria. Nucleic Acids Res. 2012 Oct;40(18):8979-92. doi: 10.1093/nar/gks694. Epub 2012 Jul 24. Lim Lab Strain N/A Add to Cart
    61161HL 1852See commentsSynthetic BiologyRegulatory consequences of gene translocation in bacteria. Nucleic Acids Res. 2012 Oct;40(18):8979-92. doi: 10.1093/nar/gks694. Epub 2012 Jul 24. Lim Lab Strain N/A Add to Cart
    61162HL 1951See commentsSynthetic BiologyRegulatory consequences of gene translocation in bacteria. Nucleic Acids Res. 2012 Oct;40(18):8979-92. doi: 10.1093/nar/gks694. Epub 2012 Jul 24. Lim Lab Strain N/A Add to Cart
    61163HL 2028See commentsSynthetic BiologyRegulatory consequences of gene translocation in bacteria. Nucleic Acids Res. 2012 Oct;40(18):8979-92. doi: 10.1093/nar/gks694. Epub 2012 Jul 24. Lim Lab Strain N/A Add to Cart
    61164HL 5220See commentsSynthetic BiologyRegulatory consequences of gene translocation in bacteria. Nucleic Acids Res. 2012 Oct;40(18):8979-92. doi: 10.1093/nar/gks694. Epub 2012 Jul 24. Lim Lab Strain N/A Add to Cart
    61165HL 5221See commentsSynthetic BiologyRegulatory consequences of gene translocation in bacteria. Nucleic Acids Res. 2012 Oct;40(18):8979-92. doi: 10.1093/nar/gks694. Epub 2012 Jul 24. Lim Lab Strain N/A Add to Cart
    61166HL 5222See commentsSynthetic BiologyRegulatory consequences of gene translocation in bacteria. Nucleic Acids Res. 2012 Oct;40(18):8979-92. doi: 10.1093/nar/gks694. Epub 2012 Jul 24. Lim Lab Strain N/A Add to Cart
    62819HL2483see commentsSynthetic BiologyParadoxical suppression of small RNA activity at high Hfq concentrations due to random-order binding. Nucleic Acids Res. 2015 Aug 10. pii: gkv777. Lim Lab strain: MG1655 + lacIq intergrated at intS + Pcon::TetR inserted into the chromosome at galK + ΔglgCAP + ΔcsrB + ΔcsrC + ΔcsrD N/A Add to Cart
    62820HL5410see commentsSynthetic BiologyParadoxical suppression of small RNA activity at high Hfq concentrations due to random-order binding. Nucleic Acids Res. 2015 Aug 10. pii: gkv777. Lim Lab strain: MG1655 + lacIq intergrated at intS + ΔglmZ + Dhfq N/A Add to Cart
    62821pHL391gfp, mCherryBacterial Expression, Synthetic BiologyParadoxical suppression of small RNA activity at high Hfq concentrations due to random-order binding. Nucleic Acids Res. 2015 Aug 10. pii: gkv777. Lim Lab plasmid: AmpR + pLlacO-1::ompC::gfp::T1T2 terminator + PLtetO-1::RBS (st7) hfq::mCherry::T1 terminator + ColE1 unknown-ColE1 ori Add to Cart
    62822pHL417gfp, micC leader, hfq leaderBacterial Expression, Synthetic BiologyParadoxical suppression of small RNA activity at high Hfq concentrations due to random-order binding. Nucleic Acids Res. 2015 Aug 10. pii: gkv777. Lim Lab plasmid: AmpR + pLlacO-1::ompC::gfp::T1T2 terminator + PLtetO-1::micC::Asp terminator + pLtetO-1::RBS (st7) hfq::T1 terminator + ColE1 unknown-ColE1 ori Add to Cart
    62823pHL518mCherry, gfpBacterial Expression, Synthetic BiologyParadoxical suppression of small RNA activity at high Hfq concentrations due to random-order binding. Nucleic Acids Res. 2015 Aug 10. pii: gkv777. Lim Lab plasmid: AmpR + PLlacO-1::micC::mCherry::T1 terminator + PLtetO-1m9::OmpC::gfp::T1T2 terminator + ColE1 unknown-ColE1 ori Add to Cart
    62825pHL671gfpBacterial Expression, Synthetic BiologyParadoxical suppression of small RNA activity at high Hfq concentrations due to random-order binding. Nucleic Acids Res. 2015 Aug 10. pii: gkv777. Lim Lab plasmid: AmpR + PCon::glgC::gfp::T1T2 terminator + ColE1 unknown-ColE1 ori Add to Cart
    62826pHL718gfp, mCherryBacterial Expression, Synthetic BiologyParadoxical suppression of small RNA activity at high Hfq concentrations due to random-order binding. Nucleic Acids Res. 2015 Aug 10. pii: gkv777. Lim Lab plasmid: AmpR + pLlacO-1::ompC::gfp::T1T2 terminator + PLtetO-1::ipex::mCherry::T1 terminator + ColE1 unknown-ColE1 ori Add to Cart
    62827pHL836gfp, ipex leader, hfq leaderBacterial Expression, Synthetic BiologyParadoxical suppression of small RNA activity at high Hfq concentrations due to random-order binding. Nucleic Acids Res. 2015 Aug 10. pii: gkv777. Lim Lab plasmid: AmpR + pLlacO-1::ompC::gfp::T1T2 terminator + PLtetO-1::ipex::Asp terminator + PLtetO-1::RBS (st7) hfq::T1 terminator + ColE1 unknown-ColE1 ori Add to Cart
    62828pHL1017gfpBacterial Expression, Synthetic BiologyParadoxical suppression of small RNA activity at high Hfq concentrations due to random-order binding. Nucleic Acids Res. 2015 Aug 10. pii: gkv777. Lim Lab plasmid: AmpR + PConNoHindM8::glgC::gfp::T1T2 terminator + ColE1 unknown-ColE1 ori Add to Cart
    62829pHL1632gfpBacterial Expression, Synthetic BiologyParadoxical suppression of small RNA activity at high Hfq concentrations due to random-order binding. Nucleic Acids Res. 2015 Aug 10. pii: gkv777. Lim Lab plasmid: AmpR + PLlacO-1::RBS(st7) gfp::Asp terminator + ColE1 unknown-ColE1 ori Add to Cart
    62830pHL1633gfpBacterial Expression, Synthetic BiologyParadoxical suppression of small RNA activity at high Hfq concentrations due to random-order binding. Nucleic Acids Res. 2015 Aug 10. pii: gkv777. Lim Lab plasmid: AmpR + PLlacO-1::T710 gfp::Asp terminator + ColE1 unknown-ColE1 ori Add to Cart
    62831pHL1954M8::ryhB leader, hfq leader, gfpBacterial Expression, Synthetic BiologyParadoxical suppression of small RNA activity at high Hfq concentrations due to random-order binding. Nucleic Acids Res. 2015 Aug 10. pii: gkv777. Lim Lab plasmid: AmpR + PconNoHindM8::ryhB::Asp terminator + PLlacO-1::RBS (st7) hfq::T1 terminator + PLtetO-1m9::sodB::gfp::T1T2 terminator + ColE1 unknown-ColE1 ori Add to Cart
    62832pHL1969M8::ryhB leader, T710 hfq leader, gfpBacterial Expression, Synthetic BiologyParadoxical suppression of small RNA activity at high Hfq concentrations due to random-order binding. Nucleic Acids Res. 2015 Aug 10. pii: gkv777. Lim Lab plasmid: AmpR + PconNoHindM8::ryhB::Asp terminator + PLlacO-1::T710 hfq::T1 terminator + PLtetO-1m9::sodB::gfp::T1T2 terminator + ColE1 unknown-ColE1 ori Add to Cart
    62833pHL2021T710 hfq leader, gfpBacterial Expression, Synthetic BiologyParadoxical suppression of small RNA activity at high Hfq concentrations due to random-order binding. Nucleic Acids Res. 2015 Aug 10. pii: gkv777. Lim Lab plasmid: AmpR + PLlacO-1::T710 hfq::T1 terminator + PLtetO-1m9::sodB::gfp::T1T2 terminator + ColE1 unknown-ColE1 ori Add to Cart
    62834pHL2026T710 hfq leader, gfpBacterial Expression, Synthetic BiologyParadoxical suppression of small RNA activity at high Hfq concentrations due to random-order binding. Nucleic Acids Res. 2015 Aug 10. pii: gkv777. Lim Lab plasmid: AmpR + PLlacO-1::T710 hfq::T1 terminator + PLtetO-1m9::glmS::gfp::T1T2 terminator + ColE1 unknown-ColE1 ori Add to Cart
    62835pHL2027T710 hfq leader, gfpBacterial Expression, Synthetic BiologyParadoxical suppression of small RNA activity at high Hfq concentrations due to random-order binding. Nucleic Acids Res. 2015 Aug 10. pii: gkv777. Lim Lab plasmid: AmpR + PLlacO-1::T710 hfq::T1terminator + PLtetO-1::glmS::gfp::T1T2 terminator + ColE1 unknown-ColE1 ori Add to Cart
    62836pHL2053T710 hfq leader, gfpBacterial Expression, Synthetic BiologyParadoxical suppression of small RNA activity at high Hfq concentrations due to random-order binding. Nucleic Acids Res. 2015 Aug 10. pii: gkv777. Lim Lab plasmid: AmpR + PLlacO-1::T710::hfq::T1 termintor + PLtetO-1m9::ompC::gfp::T1T2 terminator + ColE1 unknown-ColE1 ori Add to Cart
    62837pHL2073glmZ leader, T710 hfq leader, gfpBacterial Expression, Synthetic BiologyParadoxical suppression of small RNA activity at high Hfq concentrations due to random-order binding. Nucleic Acids Res. 2015 Aug 10. pii: gkv777. Lim Lab plasmid: AmpR + PconShortNoHind::glmZ::Asp terminator + PLlacO-1::T710 hfq::T1 terminator + PLtetO-1m9::glmS::gfp::T1T2 terminator + ColE1 unknown-ColE1 ori Add to Cart
    62838pHL2075glmZ leader, T710 hfq leader, gfpBacterial Expression, Synthetic BiologyParadoxical suppression of small RNA activity at high Hfq concentrations due to random-order binding. Nucleic Acids Res. 2015 Aug 10. pii: gkv777. Lim Lab plasmid: AmpR + PconShortNoHind::glmZ::Asp terminator + PLlacO-1::T710 hfq::T1 terminator + PLtetO-1::glmS::gfp::T1T2 terminator + ColE1 unknown-ColE1 ori Add to Cart
    62839pHL2084m8::glmZ leader, T710 hfq leader, gfpBacterial Expression, Synthetic BiologyParadoxical suppression of small RNA activity at high Hfq concentrations due to random-order binding. Nucleic Acids Res. 2015 Aug 10. pii: gkv777. Lim Lab plasmid: AmpR + PconShortNoHindM8::glmZ::Asp terminator + PLlacO-1::T710 hfq::T1 terminator + PLtetO-1m9::glmS::gfp::T1T2 terminator + ColE1 unknown-ColE1 ori Add to Cart
    62840pHL2085m8::glmZ leader, T710 hfq leader, gfpBacterial Expression, Synthetic BiologyParadoxical suppression of small RNA activity at high Hfq concentrations due to random-order binding. Nucleic Acids Res. 2015 Aug 10. pii: gkv777. Lim Lab plasmid: AmpR + PconShortNoHindM8::glmZ::Asp terminator + PLlacO-1::T710 hfq::T1 terminator + PLtetO-1::glmS::gfp::T1T2 terminator + ColE1 unknown-ColE1 ori Add to Cart
    62841pHL2107m8::micC leader, T710 hfq leader, gfpBacterial Expression, Synthetic BiologyParadoxical suppression of small RNA activity at high Hfq concentrations due to random-order binding. Nucleic Acids Res. 2015 Aug 10. pii: gkv777. Lim Lab plasmid: AmpR + PconNoHindM8::micC::Asp terminator + PLlacO-1::T710::hfq::T1 terminator + PLtetO-1m9::ompC::gfp::T1T2 terminator + ColE1 unknown-ColE1 ori Add to Cart
    62842pHL2117M8::ryhB leader, gfpBacterial Expression, Synthetic BiologyParadoxical suppression of small RNA activity at high Hfq concentrations due to random-order binding. Nucleic Acids Res. 2015 Aug 10. pii: gkv777. Lim Lab plasmid: AmpR + PconNoHindM8::ryhB::Asp terminator + PLtetO-1m9::sodB::gfp::T1T2 terminator + ColE1 unknown-ColE1 ori Add to Cart
    63667HL 5226see commentsSynthetic BiologyParadoxical suppression of small RNA activity at high Hfq concentrations due to random-order binding. Nucleic Acids Res. 2015 Aug 10. pii: gkv777. Lim Lab strain: MG1655 + lacIq intergrated at intS + ΔglmZ N/A Add to Cart
    64905pHL 275Bacterial Expression, Synthetic Biology ; E. coliQuantitative characterization of gene regulation by Rho dependent transcription termination. Biochim Biophys Acta. 2015 May 14;1849(8):940-954. doi: 10.1016/j.bbagrm.2015.05.003. KanR + ColE1 + PLlacO-1::RBS(st7):cfp::T1 terminator ColE (from pZ system) + KanR Add to Cart
    64906pHL 309Bacterial Expression, Synthetic Biology ; E. coliQuantitative characterization of gene regulation by Rho dependent transcription termination. Biochim Biophys Acta. 2015 May 14;1849(8):940-954. doi: 10.1016/j.bbagrm.2015.05.003. KanR + ColE1 + PLlacO-1::RBS(st7):rfp::RBS(st7):cfp::RBS(st7):yfp::T1 terminator ColE (from pZ system) + KanR Add to Cart
    64907pHL 886Bacterial Expression, Synthetic Biology ; E. coliQuantitative characterization of gene regulation by Rho dependent transcription termination. Biochim Biophys Acta. 2015 May 14;1849(8):940-954. doi: 10.1016/j.bbagrm.2015.05.003. KanR + ColE1 + PLlacO-1::RBS(st2):lacZ::RBS(st7):cfp::T1 terminator ColE (from pZ system) + KanR Add to Cart
    64908pHL 1988Bacterial Expression, Synthetic Biology ; E. coliQuantitative characterization of gene regulation by Rho dependent transcription termination. Biochim Biophys Acta. 2015 May 14;1849(8):940-954. doi: 10.1016/j.bbagrm.2015.05.003. KanR + ColE1 + PLlacO-1::RBS(st7):cfp::Asp terminator + PLlacO-1::RBS(st7):psu::T1 terminator ColE (from pZ system) + KanR Add to Cart
    64909pHL 2057Bacterial Expression, Synthetic Biology ; E. coliQuantitative characterization of gene regulation by Rho dependent transcription termination. Biochim Biophys Acta. 2015 May 14;1849(8):940-954. doi: 10.1016/j.bbagrm.2015.05.003. AmpR + ColE1 + PLtetO-1::RBS(st7):cro::cII (31 basepairs)::T1 terminator + PLlacO-1::RBS(st7):psu::T1 terminator ColE (from pZ system) + AmpR Add to Cart
    64910pHL 2067Bacterial Expression, Synthetic Biology ; E. coliQuantitative characterization of gene regulation by Rho dependent transcription termination. Biochim Biophys Acta. 2015 May 14;1849(8):940-954. doi: 10.1016/j.bbagrm.2015.05.003. AmpR + ColE1 + PLtetO-1:: extra 5’UTR::RBS(st7):cfp::T1 terminator + PLlacO-1::RBS(st7):psu::T1 terminator ColE (from pZ system) + AmpR Add to Cart
    69771HL372Synthetic BiologyHorizontal transfer of DNA methylation patterns into bacterial chromosomes. Nucleic Acids Res. 2016 Apr 15. pii: gkw230. MC4100 + agn43 gene deleted + ΔOxyR none Add to Cart
    69772HL444Synthetic BiologyHorizontal transfer of DNA methylation patterns into bacterial chromosomes. Nucleic Acids Res. 2016 Apr 15. pii: gkw230. MC4100 + agn43 gene deleted + Δdam none Add to Cart
    69773HL 1951Synthetic BiologyHorizontal transfer of DNA methylation patterns into bacterial chromosomes. Nucleic Acids Res. 2016 Apr 15. pii: gkw230. MG1655 + pLacO-1::T710:::YFP at GalK none Add to Cart
    69774HL 3500Synthetic BiologyHorizontal transfer of DNA methylation patterns into bacterial chromosomes. Nucleic Acids Res. 2016 Apr 15. pii: gkw230. MG1655 + pLacO-1::T710:::YFP at GalK + ΔLacI none Add to Cart
    69775HL 6686Synthetic BiologyHorizontal transfer of DNA methylation patterns into bacterial chromosomes. Nucleic Acids Res. 2016 Apr 15. pii: gkw230. MG1655 + pLacO-1::T710:::YFP at GalK + ΔLacI + Δdam none Add to Cart
    69776HL 6692Synthetic BiologyHorizontal transfer of DNA methylation patterns into bacterial chromosomes. Nucleic Acids Res. 2016 Apr 15. pii: gkw230. MG1655 + LacIq at intS + ptet::YFP::Asp terminator at galK + KanR::pT7::T710::CFP at yfjv + ptet::OmpC::T7RNAP::CamR at yeeP + ΔOxyR none Add to Cart
    69777HL 6693Synthetic BiologyHorizontal transfer of DNA methylation patterns into bacterial chromosomes. Nucleic Acids Res. 2016 Apr 15. pii: gkw230. MG1655 + LacIq at intS + ptet::YFP::Asp terminator at galK + KanR::pT7::T710::CFP at yfjv + ptet::OmpC::T7RNAP::CamR at yeeP + Δdam none Add to Cart
    69778HL 6703Synthetic BiologyHorizontal transfer of DNA methylation patterns into bacterial chromosomes. Nucleic Acids Res. 2016 Apr 15. pii: gkw230. MG1655 + pLacO-1::T710:::YFP at GalK + ΔLacI + Δdam + ΔOxyR none Add to Cart
    69779HL 6730Synthetic BiologyHorizontal transfer of DNA methylation patterns into bacterial chromosomes. Nucleic Acids Res. 2016 Apr 15. pii: gkw230. MG1655 + LacIq at intS + ptet::YFP::Asp terminator at galK + KanR::pT7::T710::CFP at yfjv + ptet::OmpC::T7RNAP::CamR at yeeP + ΔOxyR + Δdam none Add to Cart
    69780HL 6776Synthetic BiologyHorizontal transfer of DNA methylation patterns into bacterial chromosomes. Nucleic Acids Res. 2016 Apr 15. pii: gkw230. MG1655 + pLacO-1::T710:::YFP at GalK + ΔLacI + ΔSgrS none Add to Cart
    69781HL 6777Synthetic BiologyHorizontal transfer of DNA methylation patterns into bacterial chromosomes. Nucleic Acids Res. 2016 Apr 15. pii: gkw230. MG1655 + pLacO-1::T710:::YFP at GalK + ΔSgrS none Add to Cart
    69782HL 6778Synthetic BiologyHorizontal transfer of DNA methylation patterns into bacterial chromosomes. Nucleic Acids Res. 2016 Apr 15. pii: gkw230. MG1655 + pLacO-1::T710:::YFP at GalK + ΔLacI + Δdam + ΔSgrS none Add to Cart
    69783HL 6779Synthetic BiologyHorizontal transfer of DNA methylation patterns into bacterial chromosomes. Nucleic Acids Res. 2016 Apr 15. pii: gkw230. MG1655 + pLacO-1::T710:::YFP at GalK + ΔLacI + ΔOxyR none Add to Cart
    69784HL 6825Synthetic BiologyHorizontal transfer of DNA methylation patterns into bacterial chromosomes. Nucleic Acids Res. 2016 Apr 15. pii: gkw230. MG1655 + pLacO-1::T710:::YFP at GalK + ΔLacI + ΔOxyR + ΔSgrS none Add to Cart
    69785HL 6845Synthetic BiologyHorizontal transfer of DNA methylation patterns into bacterial chromosomes. Nucleic Acids Res. 2016 Apr 15. pii: gkw230. MC4100 + agn43 coding sequence replaced by T7RNAP and camR + Δdam + ΔSgrS none Add to Cart
    69786HL 6846Synthetic BiologyHorizontal transfer of DNA methylation patterns into bacterial chromosomes. Nucleic Acids Res. 2016 Apr 15. pii: gkw230. MC4100 + agn43 coding sequence replaced by T7RNAP and camR + ΔOxyR + ΔSgrS none Add to Cart
    69787pHL 1477T7RNAPBacterial Expression, Synthetic BiologyHorizontal transfer of DNA methylation patterns into bacterial chromosomes. Nucleic Acids Res. 2016 Apr 15. pii: gkw230. KanR with FRT sites + ptet::OmpC::T7RNAP::T1 terminator + ColE ColE Add to Cart
    69788pHL 1701CFPBacterial Expression, Synthetic BiologyHorizontal transfer of DNA methylation patterns into bacterial chromosomes. Nucleic Acids Res. 2016 Apr 15. pii: gkw230. KanR with FRT sites + pT7::T710::CFP::T7 terminator::T1 terminator + ColE ColE Add to Cart
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