High-throughput production of human proteins for crystallization: The SGC experience.
Savitsky P, Bray J, Cooper CD, Marsden BD, Mahajan P, Burgess-Brown NA, Gileadi O
J Struct Biol. 2010 Jun 10. ():. PubMed Article
Savitsky P, Bray J, Cooper CD, Marsden BD, Mahajan P, Burgess-Brown NA, Gileadi O
J Struct Biol. 2010 Jun 10. ():. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose | |
|---|---|---|---|
| 26103 | pNIC28-Bsa4 | SGC Empty backbone for bacterial expression |
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| 26105 | pNIC-CTHF | SGC Empty backbone for bacterial expression |
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| 26106 | pNH-TrxT | SGC Empty backbone for bacterial expression |
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| 26107 | pNIC-ZB | SGC Empty backbone for bacterial expression |
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| 26108 | pFB-LIC-Bse | SGC Baculovirus transfer vector with His tag in 22-aa N-terminal fusion peptide with TEV protease cleavage site. Includes sites for LIC cloning, and SacB gene for negative selection |
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