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USO hisB- pyrF- rpoZ-
(Bacterial strain #18049)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Bacterial Strain 18049 Bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Tetracycline
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    omega knockout Bacteria hybrid selection strain
  • Insert Size (bp)
    450
  • Mutation
    This is the US0 selection strain that has hisB, pyrF and rpoZ knocked out. A zeocin cassette has been knocked in to the rpoZ locus

Cloning Information

Resource Information

Depositor Comments

This strain has a deletion in hisB, pyrF and ropZ genes (the bacterial homologs of HIS3 and URA3 and the omega subunit) and it contains a F' episome bearing the lacl^q repressor.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    USO hisB- pyrF- rpoZ- was a gift from Scot Wolfe (Addgene plasmid # 18049)
  • For your References section:

    A systematic characterization of factors that regulate Drosophila segmentation via a bacterial one-hybrid system. Noyes MB, Meng X, Wakabayashi A, Sinha S, Brodsky MH, Wolfe SA. Nucleic Acids Res. 2008 Mar 10. ():. 10.1093/nar/gkn048 PubMed 18332042