This website uses cookies to ensure you get the best experience. By continuing the use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

Bo Huang Lab Plasmids


The Bo Huang Lab has deposited plasmids at Addgene for distribution to the research community. Addgene is a nonprofit plasmid repository dedicated to improving life science research.

Learn more about research in the Bo Huang Lab.

Addgene Alerts

Receive email alerts when new plasmids from this lab become available.

Log in to subscribe to Addgene Alerts.

Title Authors Publication
Dynamic Imaging of Genomic Loci in Living Human Cells by an Optimized CRISPR/Cas System. Chen B, Gilbert LA, Cimini BA, Schnitzbauer J, Zhang W, Li GW, Park J, Blackburn EH, Weissman JS, Qi LS, Huang B Cell. 2013 Dec 19;155(7):1479-91. doi: 10.1016/j.cell.2013.12.001.
Versatile protein tagging in cells with split fluorescent protein. Kamiyama D, Sekine S, Barsi-Rhyne B, Hu J, Chen B, Gilbert LA, Ishikawa H, Leonetti MD, Marshall WF, Weissman JS, Huang B Nat Commun. 2016 Mar 18;7:11046. doi: 10.1038/ncomms11046.
ID Plasmid Gene/Insert Vector Type Publication Hidden Extra Search Info  
51023pSLQ1658-dCas9-EGFPdCas9 fuse to EGFP (Homo sapiens)Mammalian Expression, Retroviral, CRISPRDynamic Imaging of Genomic Loci in Living Human Cells by an Optimized CRISPR/Cas System. Cell. 2013 Dec 19;155(7):1479-91. doi: 10.1016/j.cell.2013.12.001. Template for NLS-dCas9-NLS-EGFP fusion protein for CRISPR imaging (the recipient vector can be TetON 3G promoter system) pMSCVpuro Add to Cart
51024pSLQ1651-sgTelomere(F+E)Optimized sgRNA (Homo sapiens)Mammalian Expression, Lentiviral, CRISPRDynamic Imaging of Genomic Loci in Living Human Cells by an Optimized CRISPR/Cas System. Cell. 2013 Dec 19;155(7):1479-91. doi: 10.1016/j.cell.2013.12.001. Lentiviral vector that contains an optimized S. pyogenes sgRNA targeting human telomeres pSico Add to Cart
51025pSLQ1661-sgMUC4-E3(F+E)optimized sgRNA (Homo sapiens)Mammalian Expression, Lentiviral, CRISPRDynamic Imaging of Genomic Loci in Living Human Cells by an Optimized CRISPR/Cas System. Cell. 2013 Dec 19;155(7):1479-91. doi: 10.1016/j.cell.2013.12.001. Lentiviral vector that contains an optimized S. pyogenes sgRNA targeting the repetitive sequence of human MUC4 exon 3 pSico MUC4 ASGP, HSA276359, MUC-4 Add to Cart
70217pEGFP-GFP11-Clathrin light chainGFP11-Clathrin light chain (Homo sapiens)Mammalian ExpressionVersatile protein tagging in cells with split fluorescent protein. Nat Commun. 2016 Mar 18;7:11046. doi: 10.1038/ncomms11046. Expresses GFP11-clathrin light chain in mammalian cells pEGFP-mEmerald-clathrin-15 CLTA LCA Add to Cart
70218pACUH-GFP11x7-mCherry-α-tubulinGFP11x7-mCherry-α-tubulin (Drosophila melanogaster)Insect ExpressionVersatile protein tagging in cells with split fluorescent protein. Nat Commun. 2016 Mar 18;7:11046. doi: 10.1038/ncomms11046. Expresses GFP11x7-mCherry-α-tubulin in insect cells. 5 a. a. linker lengths. pACUH Add to Cart
70219pcDNA3.1-GFP(1-10)GFP(1-10) (Synthetic)Mammalian ExpressionVersatile protein tagging in cells with split fluorescent protein. Nat Commun. 2016 Mar 18;7:11046. doi: 10.1038/ncomms11046. Expresses GFP(1-10) in mammalian cells pcDNA3.1(+) Add to Cart
70220pEGFP-sfCherry11x4-ß-actinsfCherry11x4-actin (Homo sapiens)Mammalian ExpressionVersatile protein tagging in cells with split fluorescent protein. Nat Commun. 2016 Mar 18;7:11046. doi: 10.1038/ncomms11046. Expresses sfCherry11x4-ß-actin in mammalian cells pEGFP-mEmerald-actin-C-18 Add to Cart
70221pEGFP-sfCherry11-ß-actinsfCherry11-actin (Homo sapiens)Mammalian ExpressionVersatile protein tagging in cells with split fluorescent protein. Nat Commun. 2016 Mar 18;7:11046. doi: 10.1038/ncomms11046. Expresses sfCherry11-ß-actin in mammalian cells pEGFP-mEmerald-actin-C-18 Add to Cart
70222pcDNA3.1-sfCherry(1-10)sfCherry(1-10) (Synthetic)Mammalian ExpressionVersatile protein tagging in cells with split fluorescent protein. Nat Commun. 2016 Mar 18;7:11046. doi: 10.1038/ncomms11046. Expresses sfCherry(1-10) in mammalian cells pcDNA3.1(+) Add to Cart
70224pHRm-NLS-dCas9-GFP11x7-NLS-P2A-BFP-NLSNLS-dCas9-GFP11x7-NLS-P2A-BFP-NLS (Synthetic)Mammalian ExpressionVersatile protein tagging in cells with split fluorescent protein. Nat Commun. 2016 Mar 18;7:11046. doi: 10.1038/ncomms11046. Expresses NLS-dCas9-GFP11x7-NLS and BFP-NLS in mammalian cells pHRdSV40-NLS-dCas9-24xGCN4_v4-NLS-P2A-BFP-dWPRE (addgene #60910) Add to Cart
70228pHRm-GFP(1-10)-VP64-NLSGFP(1-10)-VP64-NLSMammalian ExpressionVersatile protein tagging in cells with split fluorescent protein. Nat Commun. 2016 Mar 18;7:11046. doi: 10.1038/ncomms11046. Expresses GFP(1-10)-VP64-NLS in mammalian cells pHRm Add to Cart
80409pHR-SFFV-GFP1-10GFP1-10 (Synthetic)Mammalian Expression, LentiviralVersatile protein tagging in cells with split fluorescent protein. Nat Commun. 2016 Mar 18;7:11046. doi: 10.1038/ncomms11046. Expresses GFP1-10 in mammalian cells pHR-SFFV Add to Cart