167,948 results

1. AP1muA-mStayGold-polyA-Hygro HR

   Plasmid

   #229679

   Purpose

   Homology repair plasmid for endogenous tagging of AP1muA at the C-terminus with monomeric StayGold. Contains a hygromycin resistance cassette for selection of edited cells

   Depositor

   Francesca Bottanelli

   Article

   Stockhammer et al Nat Cell Biol. 2024 Oct 4. doi: 10.1038/

   Insert

   genomic sequence AP1M1 locus (AP1M1 Human)
   

   Use

   CRISPR

   Tags

   monomeric StayGold

   Available Since

   Jan. 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSmac-GFP

   Plasmid

   #40881

   Depositor

   Douglas Green

   Article

   Tait et al Dev Cell. 2010 May 18;18(5):802-13.

   Insert

   Smac-GFP (DIABLO Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 9, 2012

   Availability

   Academic Institutions and Nonprofits only

3. pEGFP×2-N1

   Plasmid

   #86775

   Purpose

   A mammalian expression vector with double EGFP at C-terminus.

   Depositor

   Lei Lu

   Article

   Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

   Type

   Empty backbone

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCDNA3 Flag Jnk1a1

   Plasmid

   #13798

   Depositor

   Roger Davis

   Article

   Derijard et al Cell. 1994 Mar 25. 76(6):1025-37.

   Insert

   Jnk1a1 (MAPK8 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Available Since

   Feb. 22, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBHM2679

   Plasmid

   #241724

   Purpose

   Construction of fused marker-sgRNA inserts for Cryptococcus neoformans genome editing, contains BSD marker for selection on blasticidin S

   Depositor

   Hiten Madhani

   Article

   Boucher et al Microbiol Spectr. 2025 Sep 25:e0193525.

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Yeast

   Available Since

   Sept. 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBHM2681

   Plasmid

   #241726

   Purpose

   Construction of fused marker-sgRNA inserts for Cryptococcus neoformans genome editing, contains ptxD marker for selection on media containing phosphite as a sole phosphorus source

   Depositor

   Hiten Madhani

   Article

   Boucher et al Microbiol Spectr. 2025 Sep 25:e0193525.

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Yeast

   Available Since

   Sept. 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMSCVpuro-Venus

   Plasmid

   #96940

   Purpose

   Retroviral plasmid containing Venus for labeling of cells

   Depositor

   Lee Grimes

   Article

   Kleeman et al Cytometry A. 2018 Mar 13. doi: 10.1002/c

   Insert

   Venus Fluorescent Protein
   

   Expression

   Mammalian

   Available Since

   April 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. p3xFLAG SARS-CoV2 RdRp.co

   Plasmid

   #154008

   Purpose

   Expression of codon-optimized SARS-CoV2 Nsp12 (RdRp) in mammalian cells

   Depositor

   Patrick Moore

   Article

   Meng et al J Med Virol. 2023 Jan;95(1):e28246. doi:

   Insert

   Codon-optimized SARS-CoV2 Nsp12 from ORF1ab (ORF1ab Synthetic)
   

   Tags

   3xFLAG tag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 23, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pVITRO-HPV33 L1L2

   Plasmid

   #52493

   Purpose

   Expresses HPV33 L1 and L2 in mammalian cells

   Depositor

   Richard Roden

   Article

   Kwak et al PLoS One. 2014 May 9;9(5):e97232. doi: 1

   Inserts

   HPV33 L1
   HPV33 L2
   

   Expression

   Mammalian

   Available Since

   May 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV-TadA8e-BLM

    Plasmid

    #221226

    Purpose

    Express HACE Editor as a fusion of TadA-8e adenosine deaminase and BLM helicase.

    Depositor

    Fei Chen

    Article

    Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

    Insert

    TadA8e-BLM
    

    Expression

    Mammalian

    Available Since

    Sept. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDONR223_TMPO_WT

    Plasmid

    #81818

    Purpose

    Gateway Donor vector containing TMPO , part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, David Root, William Hahn

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    TMPO (TMPO Human)
    

    Use

    Gateway entry vector

    Mutation

    WT

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. PET-HiFi SpCas9-NLS-6xHis

    Plasmid

    #207376

    Purpose

    Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    HiFi SpCas9
    

    Use

    CRISPR

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    R691A

    Promoter

    T7

    Available Since

    Sept. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pX459-sgAAVS1

    Plasmid

    #184403

    Purpose

    pX459 (sgRNA and Cas9 expressing plasmid, RRID:Addgene_62988) with targeting sequence specific to AAVS1; targeting sequence is: GGGGCCACTAGGGACAGGAT

    Depositor

    Robert Bradley

    Article

    Udy et al Life Sci Alliance. 2021 Dec 8;5(3). pii:

    Insert

    AAVS1-specific sgRNA
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6 promoter

    Available Since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGL-p21UTR

    Plasmid

    #20014

    Depositor

    Robert Blelloch

    Article

    Wang et al Nat Genet. 2008 Dec . 40(12):1478-83.

    Insert

    p21 3'UTR (Cdkn1a Mouse)
    

    Use

    Luciferase

    Expression

    Mammalian

    Available Since

    Aug. 5, 2009

    Availability

    Academic Institutions and Nonprofits only

15. pAAV.CAG.iGluSnFR3.v857.PDGFR

    Plasmid

    #178333

    Purpose

    Fluorescent reporter for glutamate, third generation, variant 857. iGluSnFR3.v857

    Depositor

    Kaspar Podgorski

    Article

    Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4

    Insert

    pAAV CAG iGluSnFR3 v857.PDGFR
    

    Use

    AAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…

    Tags

    IgK-chain, Myc epi tag, and PDGFR TM Domain

    Expression

    Mammalian

    Available Since

    Feb. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. H2B-mNeonGreen-IRESpuro2

    Plasmid

    #183745

    Purpose

    Expresses H2B-mNeonGreen

    Depositor

    Daniel Gerlich

    Article

    Cuylen et al Nature. 2016 Jun 29;535(7611):308-12. do

    Insert

    H2B (H2BC16P Human)
    

    Tags

    mNeonGreen

    Expression

    Bacterial and Mammalian

    Promoter

    CMV

    Available Since

    July 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHR_pGK_LaG17_synNotch_Gal4VP64

    Plasmid

    #79127

    Purpose

    Lentiviral vector for constitutive expression of the LaG17 GFP nanobody synNotch Gal4DBDVP64 Receptor (PGK promoter)

    Depositor

    Wendell Lim

    Article

    Morsut et al Cell. 2016 Feb 11;164(4):780-91. doi: 10

    Insert

    LaG17_synNotch_Gal4DBDVP64
    

    Use

    Lentiviral

    Available Since

    July 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pOSIP-KL-mcherry

    Plasmid

    #116947

    Purpose

    Integrating mCherry into lambda attB in E. coli chromosome.

    Depositor

    David Bikard

    Article

    Cui et al Nat Commun. 2018 May 15;9(1):1912. doi:

    Insert

    mcherry
    

    Available Since

    March 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLX307-VHL(C162F)-3F-BirA

    Plasmid

    #220149

    Purpose

    Expresses VHL(C162F)-BirA as a control for VHL substrate identification by E-STUB

    Depositor

    William Sellers

    Article

    Huang et al Nat Chem Biol. 2024 Mar 21. doi: 10.1038

    Insert

    von Hippel-Lindau tumor suppressor (VHL Human)
    

    Use

    Lentiviral

    Tags

    3F-BirA

    Expression

    Mammalian

    Mutation

    changed cysteine 162 to phenylalanine; codon opti…

    Promoter

    EF-1a

    Available Since

    May 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAR028 Lenti pEF-dEnAsCas12a-NLS-ZNF705F KRAB-3XHA-T2A-BSD

    Plasmid

    #195545

    Purpose

    dCas12a effector fusion for manipulating transcription

    Depositor

    Michael Bassik

    Article

    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

    Insert

    Lenti pEF-dEnAsCas12a-NLS-TopKRAB-3XHA-T2A-BSD
    

    Use

    CRISPR and Lentiviral

    Tags

    3XHA

    Expression

    Mammalian

    Promoter

    pEF1a

    Available Since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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