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178,402 results

Showing: 2541 - 2560 of 178402 results

  1. pCMV-IRES-Renilla Luciferase-IRES-Gateway-Firefly Luciferase (pIRIGF)

    Plasmid
    #101139
    Purpose
    Luciferase reporter plasmid. Note that both renilla luciferase and ORF-firefly luciferase are encoded by a single mRNA and both are translated independently via internal ribosomal binding sites.
    Depositor
    William Kaelin
    Publication
    Lu et al Science. 2014 Jan 17;343(6168):305-9. do
    Type
    Empty backbone
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Oct. 11, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    101139-plasmid-map-sequence-id-194900

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  2. AAV:ITR-U6-sgRNA(backbone)-pEFS-Rluc-2A-Cre-WPRE-hGHpA-ITR

    Plasmid
    #60226
    Purpose
    Expresses Cre recombinase and Renilla luciferase from the EFS promoter and one U6-driven sgRNA. AAV backbone with SapI spacer for sgRNA cloning.
    Depositor
    Feng Zhang
    Publication
    Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01
    Inserts
    sgRNA
    Renilla luciferase
    Cre recombinase
    Use
    AAV, CRISPR, Cre/Lox, Luciferase, and Mouse Targeā€¦
    Tags
    Cre-HA and Rluc-P2A
    Expression
    Mammalian
    Promoter
    EFS and U6
    Available since
    Oct. 9, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    60226-plasmid-map-sequence-id-176980

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  3. pmEGFP-C1

    Plasmid
    #36412
    Depositor
    Benjamin Glick
    Type
    Empty backbone
    Tags
    mEGFP
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    May 15, 2012
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    12 Articles
    36412-plasmid-map-sequence-id-438461

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  14. Sybody Generation Toolbox

    Plasmid kit
    #1000000160
    Purpose
    Plasmids to generate synthetic nanobodies against soluble proteins, membrane proteins, and protein complexes.
    Depositor
    Markus Seeger
    Application
    Gene Expression and Labeling
    Vector type
    Bacterial Expression
    Available since
    April 15, 2020
    Availability
    Academic Institutions and Nonprofits only

  15. pET-28a(+)-FGF2-G3

    Plasmid
    #135521
    Purpose
    Engineered form of fibroblast growth factor 2 with improved thermostability
    Depositor
    Paul Burridge
    Publication
    Kuo et al Stem Cell Reports. 2020 Jan 9. pii: S221
    Insert
    fibroblast growth factor 2 (FGF2 Human)
    Tags
    6x His tag and Agg Thrombin Tag
    Expression
    Bacterial
    Mutation
    Codon optimized for E. Coli production
    Promoter
    T7 Promoter
    Available since
    Jan. 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    135521-plasmid-map-sequence-id-273044

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  16. pAAV-EF1a-DIO-ChrimsonR-mRuby2-KV2.1-WPRE-SV40 (AAV9)

    Viral prep
    #124603-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pAAV-EF1a-DIO-ChrimsonR-mRuby2-KV2.1-WPRE-SV40 (#124603). In addition to the viral particles, you will also receive purified pAAV-EF1a-DIO-ChrimsonR-mRuby2-KV2.1-WPRE-SV40 plasmid DNA. EF1a-driven, Cre-dependent expression of ChrimsonR fused to mRuby2 fluorophore and targeted to neuronal soma for optogenetic activation. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Christopher Harvey
    Publication
    Chettih et al Nature. 2019 Mar 6. pii: 10.1038/s41586-
    Tags
    mRuby2 (Cre-dependent)
    Available since
    Sept. 26, 2019
    Availability
    Academic Institutions and Nonprofits only

  17. AAV SYN flex PSAM4 GlyR IRES EGFP (AAV5)

    Viral prep
    #119741-AAV5
    Purpose
    Ready-to-use AAV5 particles produced from AAV SYN flex PSAM4 GlyR IRES EGFP (#119741). In addition to the viral particles, you will also receive purified AAV SYN flex PSAM4 GlyR IRES EGFP plasmid DNA. Synapsin-driven, Cre-dependent expression of PSAM4 GlyR IRES EGFP for neuronal silencing. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Scott Sternson
    Publication
    Magnus et al Science. 2019 Mar 14. pii: science.aav52
    Promoter
    Syn
    Tags
    EGFP (Cre-dependent)
    Available since
    April 5, 2019
    Availability
    Academic Institutions and Nonprofits only

  20. pGreenIIM DR5v2-ntdTomato/DR5-n3GFP

    Plasmid
    #61628
    Purpose
    Expresses nuclear tdTomato from auxin-dependent DR5v2 (TGTCGG) promoter and nuclear 3xGFP from the DR5 (TGTCTC) promoter
    Depositor
    Dolf Weijers
    Publication
    Liao et al Nat Methods. 2015 Mar;12(3):207-10. doi:
    Inserts
    ntdTomato
    n2xEGFP
    Expression
    Plant
    Mutation
    2 copies of EGFP with N-terminal SV40 NLS
    Promoter
    Auxin-dependent DR5 promoter containing 9 reverseā€¦
    Available since
    March 13, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    61628-plasmid-map-sequence-id-396550

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Showing: 2541 - 2560 of 178402 results