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pWS5398-CRISPRai-HygR

Plasmid

#182837

Purpose

Saccharomyces cerevisiae inducible CRISPRai vector - HygR marker

Depositor

Rodrigo Ledesma-Amaro

Article

Shaw et al Nat Commun. 2022 Aug 25;13(1):4984. doi:

Type

Empty backbone

Use

CRISPR

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pD2sE_SC14

Plasmid

#219717

Purpose

DV2 stable E dimer

Depositor

Brian Kuhlman

Article

Kudlacek et al Sci Adv. 2021 Oct 15;7(42):eabg4084. doi

Insert

DV2 sE SC14

Tags

8xHis and human serum albumin (HSA)

Expression

Mammalian

Mutation

A259W, T262R, F279W, T280P

Available Since

May 17, 2024

Availability

Academic Institutions and Nonprofits only

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pD2sE_SC14

Plasmid

#219717

Purpose

DV2 stable E dimer

Depositor

Brian Kuhlman

Article

Kudlacek et al Sci Adv. 2021 Oct 15;7(42):eabg4084. doi

Insert

DV2 sE SC14

Tags

8xHis and human serum albumin (HSA)

Expression

Mammalian

Mutation

A259W, T262R, F279W, T280P

Available Since

May 17, 2024

Availability

Academic Institutions and Nonprofits only

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pMpGWB313

Plasmid

#68641

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Tags

Glucocorticoid receptor (GR)

Expression

Plant

Promoter

MpEF1alpha promoter

Available Since

July 13, 2016

Availability

Academic Institutions and Nonprofits only

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pMpGWB313

Plasmid

#68641

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Tags

Glucocorticoid receptor (GR)

Expression

Plant

Promoter

MpEF1alpha promoter

Available Since

July 13, 2016

Availability

Academic Institutions and Nonprofits only

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px459V2.0_Conc3

Plasmid

#134458

Purpose

Empty concatemer vector in which 3 sgRNAs can be inserted; with Cas9 + Puro

Depositor

Daniel Stange

Article

Gaebler et al J Vis Exp. 2020 Feb 18;(156). doi: 10.37

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Jan. 21, 2020

Availability

Academic Institutions and Nonprofits only

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px459V2.0_Conc3

Plasmid

#134458

Purpose

Empty concatemer vector in which 3 sgRNAs can be inserted; with Cas9 + Puro

Depositor

Daniel Stange

Article

Gaebler et al J Vis Exp. 2020 Feb 18;(156). doi: 10.37

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Jan. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pSG5-hAR(16)

Plasmid

#89120

Purpose

mammalian expression of full-length human androgen receptor in pSG5, internal AR EcoRI site taken out

Depositor

Elizabeth Wilson

Article

Zhou et al Mol Endocrinol. 2002 Feb;16(2):287-300.

Insert

hAR (AR Human)

Expression

Mammalian

Mutation

internal EcoRI site removed

Promoter

SV40

Available Since

April 11, 2017

Availability

Academic Institutions and Nonprofits only
pSG5-hAR(16)

Plasmid

#89120

Purpose

mammalian expression of full-length human androgen receptor in pSG5, internal AR EcoRI site taken out

Depositor

Elizabeth Wilson

Article

Zhou et al Mol Endocrinol. 2002 Feb;16(2):287-300.

Insert

hAR (AR Human)

Expression

Mammalian

Mutation

internal EcoRI site removed

Promoter

SV40

Available Since

April 11, 2017

Availability

Academic Institutions and Nonprofits only
pB80-hsKIF5B(1-560)-L-GFP

Plasmid

#193716

Purpose

Expression of GFP tagged human kinesin-1 (KIF5B aa1-560)

Depositor

Lukas Kapitein

Article

Burute et al Sci Adv. 2022 Nov 4;8(44):eabo2343. doi:

Insert

hsKIF5B(1-560)-L-GFP (KIF5B Human, Synthetic)

Tags

EGFP

Expression

Mammalian

Mutation

hsKIF5B(aa1-560);

Promoter

Chicken beta-actin

Available Since

March 2, 2023

Availability

Academic Institutions and Nonprofits only

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pB80-hsKIF5B(1-560)-L-GFP

Plasmid

#193716

Purpose

Expression of GFP tagged human kinesin-1 (KIF5B aa1-560)

Depositor

Lukas Kapitein

Article

Burute et al Sci Adv. 2022 Nov 4;8(44):eabo2343. doi:

Insert

hsKIF5B(1-560)-L-GFP (KIF5B Human, Synthetic)

Tags

EGFP

Expression

Mammalian

Mutation

hsKIF5B(aa1-560);

Promoter

Chicken beta-actin

Available Since

March 2, 2023

Availability

Academic Institutions and Nonprofits only

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mCherry-Desmocollin1-N-18

Plasmid

#55028

Purpose

Localization: Cadherin, Excitation: 587, Emission: 610

Depositor

Michael Davidson

Insert

Desmocollin1 (DSC1 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only

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mCherry-Desmocollin1-N-18

Plasmid

#55028

Purpose

Localization: Cadherin, Excitation: 587, Emission: 610

Depositor

Michael Davidson

Insert

Desmocollin1 (DSC1 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only

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pWS3805

Plasmid

#185746

Purpose

Sub-array spacer 1/4

Depositor

Rodrigo Ledesma-Amaro

Article

Shaw et al Nat Commun. 2022 Aug 25;13(1):4984. doi:

Insert

CRISPRai sub-array spacer 1/4

Use

CRISPR

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pWS3805

Plasmid

#185746

Purpose

Sub-array spacer 1/4

Depositor

Rodrigo Ledesma-Amaro

Article

Shaw et al Nat Commun. 2022 Aug 25;13(1):4984. doi:

Insert

CRISPRai sub-array spacer 1/4

Use

CRISPR

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pLV-miRFP680-NES-p2A-dTomato-2xrGBD

Plasmid

#222638

Purpose

Rho reporter

Depositor

Arnold Hayer

Article

Marshall-Burghardt et al Sci Adv. 2024 Sep 6;10(36):eadn6858. doi

Insert

miRFP680-NES-p2A-dTomato-2xrGBD

Use

Lentiviral

Promoter

EF1a

Available Since

July 23, 2024

Availability

Academic Institutions and Nonprofits only

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pLV-miRFP680-NES-p2A-dTomato-2xrGBD

Plasmid

#222638

Purpose

Rho reporter

Depositor

Arnold Hayer

Article

Marshall-Burghardt et al Sci Adv. 2024 Sep 6;10(36):eadn6858. doi

Insert

miRFP680-NES-p2A-dTomato-2xrGBD

Use

Lentiviral

Promoter

EF1a

Available Since

July 23, 2024

Availability

Academic Institutions and Nonprofits only

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pLV_mTurquoise2-iLID-CAAX

Plasmid

#160999

Purpose

Mammalian expression of mTurquoise2-tagged iLID anchored to the plasma membrane via C-terminal fusion to CAAX motif from KRAS

Depositor

Sean R Collins

Article

Natwick et al ACS Synth Biol. 2021 May 21;10(5):1009-1

Insert

mTurquoise2-iLID-CAAX

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pLV_mTurquoise2-iLID-CAAX

Plasmid

#160999

Purpose

Mammalian expression of mTurquoise2-tagged iLID anchored to the plasma membrane via C-terminal fusion to CAAX motif from KRAS

Depositor

Sean R Collins

Article

Natwick et al ACS Synth Biol. 2021 May 21;10(5):1009-1

Insert

mTurquoise2-iLID-CAAX

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pCR4-huNFATc1nuc

Plasmid

#23988

Depositor

Jerry Crabtree

Article

Winslow et al Dev Cell. 2006 Jun . 10(6):771-82.

Insert

NFATc1 (NFATC1 Human)

Use

Cloning vector

Tags

EE tag

Mutation

S172A S175A S176A S178A S179A S181A S184A S187A S…

Available Since

March 24, 2010

Availability

Academic Institutions and Nonprofits only

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