Engineering the AAVS1 locus for consistent and scalable transgene expression in human iPSCs and their differentiated derivatives.
Oceguera-Yanez F, Kim SI, Matsumoto T, Tan GW, Xiang L, Hatani T, Kondo T, Ikeya M, Yoshida Y, Inoue H, Woltjen K
Methods. 2015 Dec 18. pii: S1046-2023(15)30181-X. doi: 10.1016/j.ymeth.2015.12.012. PubMed Article
Oceguera-Yanez F, Kim SI, Matsumoto T, Tan GW, Xiang L, Hatani T, Kondo T, Ikeya M, Yoshida Y, Inoue H, Woltjen K
Methods. 2015 Dec 18. pii: S1046-2023(15)30181-X. doi: 10.1016/j.ymeth.2015.12.012. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 80487 | pAAVS1-Nst-MCS | donor vector for AAVS1 targeting (neomycin selection) with multiple cloning site |
| 80488 | pAAVS1-P-MCS | donor vector for AAVS1 targeting (puromycin selection) with multiple cloning site |
| 80489 | pAAVS1-Nst-CAG-DEST | Gateway Destination donor vector for AAVS1 targeting (puromycin selection) |
| 80490 | pAAVS1-P-CAG-DEST | Gateway Destination donor vector for AAVS1 targeting (puromycin selection) |
| 80491 | pAAVS1-P-CAG-GFP | donor vector for AAVS1 targeting (puromycin selection) and constitutive GFP expression |
| 80492 | pAAVS1-P-CAG-mCh | donor vector for AAVS1 targeting (puromycin selection) and constitutive mCherry expression |
| 80493 | pAAVS1-P-CAG-GFPluc2 | donor vector for AAVS1 targeting (puromycin selection) and constitutive GFP-luciferase fusion protein expression |
| 80494 | pXAT2 | AAVS1 sgRNA expression vector |
| 80495 | pTALdNC-AAVS1_T1 | AAVS1 TALEN expression vector (Left) |
| 80496 | pTALdNC-AAVS1_T2 | AAVS1 TALEN expression vector (Right) |