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pU6-(BbsI)_CBh-Cas9-T2A-BFP-P2A-Ad4E1B

Plasmid

#64218

Purpose

Expression vector for sgRNA and for Expression of Cas9 linked to BFP via T2A linked to Ad4 E1B via P2A

Depositor

Ralf Kuehn

Publication

Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103

Inserts

Cas9
sgRNA cassette

Use

CRISPR

Tags

3xFLAG, NLS, and T2A-BFP-P2A-E1B

Expression

Mammalian

Promoter

CBh and U6

Available since

May 19, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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mTFP1-N1

Plasmid

#54521

Purpose

Localization: N1 Cloning Vector, Excitation: 462, Emission: 492

Depositor

Robert Campbell, Michael Davidson

Publication

Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

Type

Empty backbone

Tags

mTFP1

Expression

Mammalian

Available since

June 20, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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AmCyan-P2A-mCherry

Plasmid

#45350

Depositor

Ilpo Huhtaniemi

Publication

Potorac et al J Endocrinol. 2016 Sep 21. pii: JOE-16-0

Insert

AmCyan

Tags

mCherry

Expression

Mammalian

Mutation

SV40 NLS

Promoter

CMV

Available since

Nov. 6, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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LeGO-iG2

Plasmid

#27341

Depositor

Boris Fehse

Publication

Weber et al Mol Ther. 2008 Apr . 16(4):698-706.

Insert

SFFV promoter, IRES-eGFP

Use

Lentiviral

Expression

Mammalian

Available since

April 24, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

41 Articles

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myc-hULK1

Plasmid

#31961

Depositor

Do-Hyung Kim

Publication

Jung et al Mol Biol Cell. 2009 Apr;20(7):1992-2003.

Insert

ULK1 (ULK1 Human)

Tags

myc

Expression

Mammalian

Promoter

CMV, SP6

Available since

Sept. 1, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

16 Articles

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Lenti-sh1368 knockdown c-myc

Plasmid

#29435

Depositor

Bob Eisenman

Publication

Lin et al EMBO J. 2009 Oct 21. 28(20):3157-70.

Insert

c-Myc shRNA (MYC Human)

Use

Lentiviral and RNAi

Tags

GFP and WPRE

Expression

Mammalian

Available since

May 6, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

14 Articles

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MSCV

Plasmid

#24828

Depositor

Lin He

Publication

Olive et al Genes Dev. 2009 Dec 15. 23(24):2839-49.

Type

Empty backbone

Use

Retroviral

Expression

Mammalian

Available since

June 10, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

18 Articles

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3XFlag pCMV5-TOPO TAZ WT

Plasmid

#24809

Depositor

Jeff Wrana

Publication

Varelas et al Nat Cell Biol. 2008 Jul . 10(7):837-48.

Insert

TAZ (WWTR1 Human)

Tags

3XFlag

Expression

Mammalian

Available since

May 14, 2010

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

12 Articles

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pAc-Neo-OVA

Plasmid

#22533

Depositor

Michael Bevan

Publication

Moore et al Cell. 1988 Sep 9. 54(6):777-85.

Insert

OVA (OVAL Chicken)

Expression

Mammalian

Available since

Dec. 28, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pAL175

Plasmid

#22276

Purpose

Expresses YFP-PIF for red-light-induced binding to Phy

Depositor

Christopher Voigt

Publication

Levskaya et al Nature. 2009 Oct 15;461(7266):997-1001.

Insert

PIF6APB (PIL2 Mustard Weed)

Use

Synthetic Biology

Tags

mYFP

Expression

Mammalian

Promoter

SV40

Available since

Oct. 21, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pGL2Basic-EcadK1

Plasmid

#19290

Depositor

Eric Fearon

Publication

Hajra et al Oncogene. 1999 Dec 2. 18(51):7274-9.

Insert

E-cadherin

Use

Luciferase

Expression

Mammalian

Mutation

E-cad promoter sequences from -108 to +125

Available since

Sept. 19, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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MSCV PIG (Puro IRES GFP)

Plasmid

#18751

Depositor

Scott Lowe

Type

Empty backbone

Use

Retroviral

Expression

Mammalian

Available since

July 3, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

34 Articles

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EF.PGK.GFP

Plasmid

#17618

Depositor

Linzhao Cheng

Publication

Yu et al Mol Ther. 2003 Jun . 7(6):827-38.

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available since

March 27, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pMIG-hOCT4

Plasmid

#17225

Depositor

George Daley

Publication

Park et al Nature. 2008 Jan 10. 451(7175):141-6.

Insert

POU class 5 homeobox 1 (POU5F1 Human)

Use

Retroviral

Expression

Mammalian

Available since

Jan. 18, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

15 Articles

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pcDNA3-DN-hCUL4A-FLAG

Plasmid

#15821

Depositor

Wade Harper

Publication

Jin et al Methods Enzymol. 2005;399:287-309. doi:

Insert

Cullin4A (CUL4A Human)

Tags

Flag

Expression

Mammalian

Mutation

Dominant negative: N440

Available since

Sept. 28, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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HA-CYLD-WT

Plasmid

#15506

Depositor

Stephen Elledge

Publication

Stegmeier et al Proc Natl Acad Sci U S A. 2007 May 22. 1

Insert

CYLD (CYLD Human)

Tags

HA

Expression

Mammalian

Available since

Aug. 13, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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tFucci(SA)5

Plasmid

#153520

Purpose

New color variant of Fucci (Fluorescent ubiquitination-based cell cycle indicator) : Fucci(SA)5.

Depositor

Atsushi Miyawaki

Publication

Ando et al Cell Struct Funct. 2023 Jun 30. doi: 10.

Insert

tFucci(SA)5.

Tags

AzaleaB5-hCdt1(30/120)_P2A_h2-3-hGem(1/110)

Expression

Bacterial and Mammalian

Promoter

CAG

Available since

Aug. 20, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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mPiezo2-CMV-Sport6

Plasmid

#81073

Purpose

mouse Piezo2

Depositor

Ardem Patapoutian

Publication

Coste et al Nat Commun. 2015 May 26;6:7223. doi: 10.

Insert

Piezo2 (please see depositor comments below) (Piezo2 Mouse)

Expression

Mammalian

Available since

Nov. 18, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pMIR-FLAG-MLL-AF9

Plasmid

#71444

Purpose

Retroviral construct to express FLAG-tagged MLL-AF9 fusion gene, followed by IRES-dsRed Express2

Depositor

Daisuke Nakada

Publication

Saito et al Cell Stem Cell. 2015 Sep 29. pii: S1934-

Insert

MLL-AF9 (KMT2A Human)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Promoter

MSCV

Available since

Jan. 25, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pBabe-Kras G12C

Plasmid

#58901

Purpose

Expresses human Kras with a G12C mutation

Depositor

Channing Der

Insert

KRAS (KRAS Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

G12C mutation

Available since

Sept. 15, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

13 Articles

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