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Engineering Customized Cell Sensing and Response Behaviors Using Synthetic Notch Receptors.
Morsut L, Roybal KT, Xiong X, Gordley RM, Coyle SM, Thomson M, Lim WA
Cell. 2016 Feb 11;164(4):780-91. doi: 10.1016/j.cell.2016.01.012. Epub 2016 Jan 28.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
79119pHR_5x Gal4 UASBase vector for cloning Gal4DBDVP64 inducible genes
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79120pHR_PGKLentiviral vector for constitutive expression of genes (PGK promoter)
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79121pHR_SFFVLentiviral vector for constitutive expression of genes (SFFV promoter)
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79123pHR_Gal4UAS_IRES_mC_pGK_tBFPLentiviral vector for inducible expression of genes with Gal4DBDVP64 synNotch Receptors
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79124pHR_Gal4UAS_pGK_mCherryLentiviral vector for inducible expression of genes with Gal4DBDVP64 synNotch Receptors (no IRES)
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79125pHR_PGK_antiCD19_synNotch_Gal4VP64Lentiviral vector for constitutive expression of the anti-CD19 scFv synNotch Gal4DBDVP64 Receptor (PGK promoter)
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79126pHR_PGK_antiCD19_synNotch_TetRVP64Lentiviral vector for constitutive expression of the anti-CD19 scFv synNotch TetRVP64 Receptor (PGK promoter)
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79127pHR_pGK_LaG17_synNotch_Gal4VP64Lentiviral vector for constitutive expression of the LaG17 GFP nanobody synNotch Gal4DBDVP64 Receptor (PGK promoter)
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79128pHR_SFFV_LaG17_synNotch_TetRVP64Lentiviral vector for constitutive expression of the LaG17 GFP nanobody synNotch TetRVP64 Receptor (SFFV promoter)
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79129pHR_EGFPligandLentiviral vector for surface expressed EGFP (SFFV promoter)
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79130pHR_Gal4UAS_tBFP_PGK_mCherryLentiviral vector - tBFP reporter for Gal4DBDVP64 synNotch receptors with a constitutive mCherry
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