168,037 results

1. pCas9-sgAAVS1-2

   Plasmid

   #129727

   Purpose

   Expressing SpCas9 and sgAAVS1-2

   Depositor

   Elina Ikonen

   Article

   Li et al Nat Methods. 2019 Aug 26. pii: 10.1038/s

   Insert

   SpCas9 and sgAAVS1-2
   

   Use

   CRISPR

   Tags

   T2A-mCherry-P2A-Puro

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   Aug. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMB1-A

   Plasmid

   #190114

   Purpose

   Level 1 MoClo plasmid for A. baumannii

   Depositor

   Veronica Godoy-Carter

   Article

   Brychcy et al ACS Synth Biol. 2023 Aug 16. doi: 10.102

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   Aug. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pVITRO1-mCardinal-CAXX

   Plasmid

   #184451

   Purpose

   Plasma membrane-targeted mCardinal expression in mammalian cells

   Depositor

   Chuan-Hsiang Huang

   Article

   Yang et al Cell. 2021 Dec 9;184(25):6193-6206.e14.

   Insert

   mCardinal-CAAX
   

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Rat EF1α

   Available Since

   May 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV-T7-SpRY-P2A-EGFP (RTW4830)

   Plasmid

   #139989

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized SpRY(A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Walton et al Science. 2020 Apr 17;368(6488):290-296.

   Insert

   human codon optimized SpCas9 variant named SpRY with BPNLS-3xFLAG-P2A-EGFP
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS-3xFLAG-P2A-EGFP

   Expression

   Mammalian

   Mutation

   SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N131…

   Promoter

   CMV and T7

   Available Since

   March 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAF0451 Si74_attP-mCherry donor

   Plasmid

   #193470

   Purpose

   Promoterless mcherry donor for Si74

   Depositor

   Patrick Hsu

   Article

   Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

   Insert

   Si74_attP
   

   Expression

   Mammalian

   Available Since

   Nov. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. 1GFP/RNase H1 D210N

   Plasmid

   #174448

   Purpose

   Express GFP-tagged RNase H1 D210N in E. coli.

   Depositor

   Karlene Cimprich

   Article

   Crossley et al J Cell Biol. 2021 Sep 6;220(9). pii: 212

   Insert

   RNase H1 (RNASEH1 Human)
   

   Tags

   His-tag, GFP

   Expression

   Bacterial

   Mutation

   D210N

   Promoter

   T7 promoter

   Available Since

   Sept. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGI3EM20C

   Plasmid

   #135487

   Purpose

   Agrobacterium T-DNA with divergent Spun H2A/Bpr driving H2B-tdTomato and hph

   Depositor

   Nicolas Buchler

   Article

   Medina et al Elife. 2020 May 11;9. pii: 52741. doi: 1

   Insert

   hph SpunH2A/Bpr H2B-tdTomato
   

   Tags

   tdTomato

   Expression

   Bacterial and Yeast

   Promoter

   SpunH2A/Bpr (divergent)

   Available Since

   May 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. Nb-gp41-Halo (MoonTag-Nb-Halo)

   Plasmid

   #128603

   Purpose

   Expresses nanobody-2H10 fused to Halo in mammalian cells. It recognizes gp41 peptide array (MoonTag peptide array).

   Depositor

   Marvin Tanenbaum

   Article

   Boersma et al Cell. 2019 Jun 5. pii: S0092-8674(19)304

   Insert

   Nanobody_gp41
   

   Use

   Lentiviral

   Tags

   GB1 and Halo

   Promoter

   SFFV

   Available Since

   Aug. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. huntingtin_FL_NbioCflag

   Plasmid

   #194767

   Purpose

   Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cells

   Depositor

   Cheryl Arrowsmith

   Insert

   HTT (HTT Human)
   

   Use

   Baculovirus expression

   Tags

   Avi-tag (biotin acceptor peptide) and Flag-tag

   Expression

   Mammalian

   Mutation

   Q23 (polyQ repeat)

   Promoter

   CMV and P10

   Available Since

   July 27, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pLX313-Firefly luciferase

    Plasmid

    #118017

    Purpose

    Constitutive expression of Firefly luciferase

    Depositor

    William Hahn, David Root

    Article

    Giacomelli et al Nat Genet. 2018 Oct;50(10):1381-1387. do

    Insert

    Firefly luciferase
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1alpha

    Available Since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. FTL

    Plasmid

    #100750

    Purpose

    Part of FerriTag expression system. Untagged Ferritin light chain in mammalian expression vector.

    Depositor

    Stephen Royle

    Article

    Clarke et al Nat Commun. 2018 Jul 4;9(1):2604. doi: 1

    Insert

    FTL (FTL Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGP-AAV-syn-FLEX-jGCaMP8m-WPRE (AAV1)

    Viral Prep

    #162378-AAV1

    Purpose

    Ready-to-use AAV1 particles produced from pGP-AAV-syn-FLEX-jGCaMP8m-WPRE (#162378). In addition to the viral particles, you will also receive purified pGP-AAV-syn-FLEX-jGCaMP8m-WPRE plasmid DNA. Syn-driven, Cre-dependent expression of calcium sensor GCaMP8m (faster, more sensitive). These AAV preparations are suitable purity for injection into animals.

    Depositor

    GENIE Project

    Article

    Zhang et al Janelia Research Campus, Online Resource

    Promoter

    Syn

    Available Since

    April 26, 2021

    Availability

    Academic Institutions and Nonprofits only

13. pAF0474 Ef1a-Si74_attB acceptor

    Plasmid

    #193475

    Purpose

    Acceptor plasmid for 3 plasmid recombination assay

    Depositor

    Patrick Hsu

    Article

    Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

    Insert

    Si74_attB
    

    Expression

    Mammalian

    Promoter

    Ef1a

    Available Since

    Dec. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pNeonGreen-C1-Nir2-LNS2

    Plasmid

    #220086

    Purpose

    C-terminal PA binding domain of Nir2

    Depositor

    Gerry Hammond

    Article

    Weckerly et al J Cell Biol. 2025 Nov 3;224(11):e2024051

    Insert

    PITPNM1 (PITPNM1 Human)
    

    Tags

    NeonGreen-GGSGGM

    Expression

    Mammalian

    Mutation

    amino acids 896-1216

    Promoter

    CMV

    Available Since

    May 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLenti CMV Puro DEST (w118-1)

    Plasmid

    #17452

    Purpose

    3rd gen lentiviral Gateway destination vector, expression, CMV promoter, Puro

    Depositor

    Eric Campeau, Paul Kaufman

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral; Destination vector

    Expression

    Mammalian

    Available Since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLIB

    Plasmid

    #80610

    Purpose

    pLIB - Library vector (biGBac system for expression of protein complexes in insect cells)

    Depositor

    Jan-Michael Peters

    Article

    Weissmann et al Proc Natl Acad Sci U S A. 2016 May 10;11

    Type

    Empty backbone

    Expression

    Insect

    Promoter

    polyhedrin

    Available Since

    Oct. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. PCDNA3-GFP1-9

    Plasmid

    #121684

    Purpose

    PPI reporter

    Depositor

    Xiaokun Shu

    Article

    Zhang et al Protein Sci. 2018 Apr;27(4):874-879. doi

    Insert

    GFP1-9
    

    Expression

    Mammalian

    Available Since

    Feb. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET28-MBP-super TEV protease

    Plasmid

    #171782

    Purpose

    Expresses Maltose-binding protein-super TEV protease in bacterial cytoplasm. This protease version performs well in the absence of added reducing agent.

    Depositor

    Mark Howarth

    Article

    Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-

    Insert

    MBP-sTEV
    

    Tags

    Arg6 tag and Internal His6 tag

    Expression

    Bacterial

    Mutation

    No internal TEV cleavage site between the MBP and…

    Promoter

    T7

    Available Since

    July 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMKO.1 GFP

    Plasmid

    #10676

    Purpose

    Retroviral shRNA vector. Derivative of pMKO.1 puro (Addgene plasmid #8452) with GFP instead of puromycin resistance gene.

    Depositor

    William Hahn

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    RNAi and Retroviral

    Expression

    Mammalian

    Available Since

    Dec. 7, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSicoR-eIF1a-GFP-RPL10a

    Plasmid

    #159890

    Purpose

    Expresses GFP-RPL10a for ribosomal immunoprecipitation

    Depositor

    James Ellis

    Article

    Rodrigues et al Cell Rep. 2020 Mar 24;30(12):4179-4196.e

    Insert

    RPL10a (RPL10A Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Promoter

    eIF1a

    Available Since

    Sept. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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