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pLX_311-Cas9 Citations (25)

Originally described in: Rational design of highly active sgRNAs for CRISPR-Cas9-mediated gene inactivation.
Doench JG, Hartenian E, Graham DB, Tothova Z, Hegde M, Smith I, Sullender M, Ebert BL, Xavier RJ, Root DE Nat Biotechnol. 2014 Sep 3. doi: 10.1038/nbt.3026. PubMed Journal

Articles Citing pLX_311-Cas9

Articles
Evaluation of RNAi and CRISPR technologies by large-scale gene expression profiling in the Connectivity Map. Smith I, Greenside PG, Natoli T, Lahr DL, Wadden D, Tirosh I, Narayan R, Root DE, Golub TR, Subramanian A, Doench JG. PLoS Biol. 2017 Nov 30;15(11):e2003213. doi: 10.1371/journal.pbio.2003213. eCollection 2017 Nov. PubMed
Uncoupling of sgRNAs from their associated barcodes during PCR amplification of combinatorial CRISPR screens. Hegde M, Strand C, Hanna RE, Doench JG. PLoS One. 2018 May 25;13(5):e0197547. doi: 10.1371/journal.pone.0197547. eCollection 2018. PubMed
Optimized libraries for CRISPR-Cas9 genetic screens with multiple modalities. Sanson KR, Hanna RE, Hegde M, Donovan KF, Strand C, Sullender ME, Vaimberg EW, Goodale A, Root DE, Piccioni F, Doench JG. Nat Commun. 2018 Dec 21;9(1):5416. doi: 10.1038/s41467-018-07901-8. PubMed

Associated Plasmids
Small-molecule targeting of brachyury transcription factor addiction in chordoma. Sharifnia T, Wawer MJ, Chen T, Huang QY, Weir BA, Sizemore A, Lawlor MA, Goodale A, Cowley GS, Vazquez F, Ott CJ, Francis JM, Sassi S, Cogswell P, Sheppard HE, Zhang T, Gray NS, Clarke PA, Blagg J, Workman P, Sommer J, Hornicek F, Root DE, Hahn WC, Bradner JE, Wong KK, Clemons PA, Lin CY, Kotz JD, Schreiber SL. Nat Med. 2019 Feb;25(2):292-300. doi: 10.1038/s41591-018-0312-3. Epub 2019 Jan 21. PubMed
A GPX4-dependent cancer cell state underlies the clear-cell morphology and confers sensitivity to ferroptosis. Zou Y, Palte MJ, Deik AA, Li H, Eaton JK, Wang W, Tseng YY, Deasy R, Kost-Alimova M, Dancik V, Leshchiner ES, Viswanathan VS, Signoretti S, Choueiri TK, Boehm JS, Wagner BK, Doench JG, Clish CB, Clemons PA, Schreiber SL. Nat Commun. 2019 Apr 8;10(1):1617. doi: 10.1038/s41467-019-09277-9. PubMed
6-Phosphogluconate Dehydrogenase Links Cytosolic Carbohydrate Metabolism to Protein Secretion via Modulation of Glutathione Levels. Li H, Ericsson M, Rabasha B, Budnik B, Chan SH, Freinkman E, Lewis CA, Doench JG, Wagner BK, Garraway LA, Schreiber SL. Cell Chem Biol. 2019 Sep 19;26(9):1306-1314.e5. doi: 10.1016/j.chembiol.2019.05.006. Epub 2019 Jun 13. PubMed
Identification of functional regulatory elements in the human genome using pooled CRISPR screens. Borys SM, Younger ST. BMC Genomics. 2020 Jan 31;21(1):107. doi: 10.1186/s12864-020-6497-0. PubMed
Cytochrome P450 oxidoreductase contributes to phospholipid peroxidation in ferroptosis. Zou Y, Li H, Graham ET, Deik AA, Eaton JK, Wang W, Sandoval-Gomez G, Clish CB, Doench JG, Schreiber SL. Nat Chem Biol. 2020 Mar;16(3):302-309. doi: 10.1038/s41589-020-0472-6. Epub 2020 Feb 17. PubMed
A widespread family of heat-resistant obscure (Hero) proteins protect against protein instability and aggregation. Tsuboyama K, Osaki T, Matsuura-Suzuki E, Kozuka-Hata H, Okada Y, Oyama M, Ikeuchi Y, Iwasaki S, Tomari Y. PLoS Biol. 2020 Mar 12;18(3):e3000632. doi: 10.1371/journal.pbio.3000632. eCollection 2020 Mar. PubMed

Associated Plasmids
Genome-wide CRISPR Screens Reveal Host Factors Critical for SARS-CoV-2 Infection. Wei J, Alfajaro MM, DeWeirdt PC, Hanna RE, Lu-Culligan WJ, Cai WL, Strine MS, Zhang SM, Graziano VR, Schmitz CO, Chen JS, Mankowski MC, Filler RB, Ravindra NG, Gasque V, de Miguel FJ, Patil A, Chen H, Oguntuyo KY, Abriola L, Surovtseva YV, Orchard RC, Lee B, Lindenbach BD, Politi K, van Dijk D, Kadoch C, Simon MD, Yan Q, Doench JG, Wilen CB. Cell. 2021 Jan 7;184(1):76-91.e13. doi: 10.1016/j.cell.2020.10.028. Epub 2020 Oct 20. PubMed

Associated Plasmids
Cohesin mutations alter DNA damage repair and chromatin structure and create therapeutic vulnerabilities in MDS/AML. Tothova Z, Valton AL, Gorelov RA, Vallurupalli M, Krill-Burger JM, Holmes A, Landers CC, Haydu JE, Malolepsza E, Hartigan C, Donahue M, Popova KD, Koochaki S, Venev SV, Rivera J, Chen E, Lage K, Schenone M, D'Andrea AD, Carr SA, Morgan EA, Dekker J, Ebert BL. JCI Insight. 2021 Feb 8;6(3). pii: 142149. doi: 10.1172/jci.insight.142149. PubMed
Functional Genomics Identify Distinct and Overlapping Genes Mediating Resistance to Different Classes of Heterobifunctional Degraders of Oncoproteins. Shirasaki R, Matthews GM, Gandolfi S, de Matos Simoes R, Buckley DL, Raja Vora J, Sievers QL, Bruggenthies JB, Dashevsky O, Poarch H, Tang H, Bariteau MA, Sheffer M, Hu Y, Downey-Kopyscinski SL, Hengeveld PJ, Glassner BJ, Dhimolea E, Ott CJ, Zhang T, Kwiatkowski NP, Laubach JP, Schlossman RL, Richardson PG, Culhane AC, Groen RWJ, Fischer ES, Vazquez F, Tsherniak A, Hahn WC, Levy J, Auclair D, Licht JD, Keats JJ, Boise LH, Ebert BL, Bradner JE, Gray NS, Mitsiades CS. Cell Rep. 2021 Jan 5;34(1):108532. doi: 10.1016/j.celrep.2020.108532. PubMed
SARS-CoV-2 triggers an MDA-5-dependent interferon response which is unable to control replication in lung epithelial cells. Rebendenne A, Valadao ALC, Tauziet M, Maarifi G, Bonaventure B, McKellar J, Planes R, Nisole S, Arnaud-Arnould M, Moncorge O, Goujon C. J Virol. 2021 Jan 29. pii: JVI.02415-20. doi: 10.1128/JVI.02415-20. PubMed

Associated Plasmids
Bidirectional genome-wide CRISPR screens reveal host factors regulating SARS-CoV-2, MERS-CoV and seasonal HCoVs. Goujon C, Rebendenne A, Roy P, Bonaventure B, Valadao AC, Desmarets L, Rouille Y, Tauziet M, Arnaud-Arnould M, Giovannini D, Lee Y, DeWeirdt P, Hegde M, de Gracia FG, McKellar J, Wencker M, Dubuisson J, Belouzard S, Moncorge O, Doench J. Res Sq. 2021 May 27. doi: 10.21203/rs.3.rs-555275/v1. PubMed
Enhanced prime editing systems by manipulating cellular determinants of editing outcomes. Chen PJ, Hussmann JA, Yan J, Knipping F, Ravisankar P, Chen PF, Chen C, Nelson JW, Newby GA, Sahin M, Osborn MJ, Weissman JS, Adamson B, Liu DR. Cell. 2021 Oct 28;184(22):5635-5652.e29. doi: 10.1016/j.cell.2021.09.018. PubMed

Associated Plasmids
S-acylation by ZDHHC20 targets ORAI1 channels to lipid rafts for efficient Ca(2+) signaling by Jurkat T cell receptors at the immune synapse. Carreras-Sureda A, Abrami L, Ji-Hee K, Wang WA, Henry C, Frieden M, Didier M, van der Goot FG, Demaurex N. Elife. 2021 Dec 16;10. pii: 72051. doi: 10.7554/eLife.72051. PubMed
p53 Promotes Cytokine Expression in Melanoma to Regulate Drug Resistance and Migration. Pandya P, Kublo L, Stewart-Ornstein J. Cells. 2022 Jan 25;11(3). pii: cells11030405. doi: 10.3390/cells11030405. PubMed
Comparative optimization of combinatorial CRISPR screens. Li R, Klingbeil O, Monducci D, Young MJ, Rodriguez DJ, Bayyat Z, Dempster JM, Kesar D, Yang X, Zamanighomi M, Vakoc CR, Ito T, Sellers WR. Nat Commun. 2022 May 5;13(1):2469. doi: 10.1038/s41467-022-30196-9. PubMed

Associated Plasmids
LPCAT3 Inhibitors Remodel the Polyunsaturated Phospholipid Content of Human Cells and Protect from Ferroptosis. Reed A, Ichu TA, Milosevich N, Melillo B, Schafroth MA, Otsuka Y, Scampavia L, Spicer TP, Cravatt BF. ACS Chem Biol. 2022 Jun 17;17(6):1607-1618. doi: 10.1021/acschembio.2c00317. Epub 2022 Jun 6. PubMed
Bidirectional genome-wide CRISPR screens reveal host factors regulating SARS-CoV-2, MERS-CoV and seasonal HCoVs. Rebendenne A, Roy P, Bonaventure B, Chaves Valadao AL, Desmarets L, Arnaud-Arnould M, Rouille Y, Tauziet M, Giovannini D, Touhami J, Lee Y, DeWeirdt P, Hegde M, Urbach S, Koulali KE, de Gracia FG, McKellar J, Dubuisson J, Wencker M, Belouzard S, Moncorge O, Doench JG, Goujon C. Nat Genet. 2022 Aug;54(8):1090-1102. doi: 10.1038/s41588-022-01110-2. Epub 2022 Jul 25. PubMed
Accounting for small variations in the tracrRNA sequence improves sgRNA activity predictions for CRISPR screening. DeWeirdt PC, McGee AV, Zheng F, Nwolah I, Hegde M, Doench JG. Nat Commun. 2022 Sep 6;13(1):5255. doi: 10.1038/s41467-022-33024-2. PubMed
Chromatin complex dependencies reveal targeting opportunities in leukemia. Najm FJ, DeWeirdt P, Moore MM, Bevill SM, El Farran CA, Macias KA, Hegde M, Waterbury AL, Liau BB, van Galen P, Doench JG, Bernstein BE. Nat Commun. 2023 Jan 27;14(1):448. doi: 10.1038/s41467-023-36150-7. PubMed
TMEM164 is an acyltransferase that forms ferroptotic C20:4 ether phospholipids. Reed A, Ware T, Li H, Fernando Bazan J, Cravatt BF. Nat Chem Biol. 2023 Mar;19(3):378-388. doi: 10.1038/s41589-022-01253-7. Epub 2023 Feb 13. PubMed
Mapping the landscape of genetic dependencies in chordoma. Sharifnia T, Wawer MJ, Goodale A, Lee Y, Kazachkova M, Dempster JM, Muller S, Levy J, Freed DM, Sommer J, Kalfon J, Vazquez F, Hahn WC, Root DE, Clemons PA, Schreiber SL. Nat Commun. 2023 Apr 6;14(1):1933. doi: 10.1038/s41467-023-37593-8. PubMed
The ER stress sensor IRE1 interacts with STIM1 to promote store-operated calcium entry, T cell activation, and muscular differentiation. Carreras-Sureda A, Zhang X, Laubry L, Brunetti J, Koenig S, Wang X, Castelbou C, Hetz C, Liu Y, Frieden M, Demaurex N. Cell Rep. 2023 Dec 26;42(12):113540. doi: 10.1016/j.celrep.2023.113540. Epub 2023 Dec 5. PubMed

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