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RRL.sin.cPPT.SFFV/TMPRSS2 (variant 1).IRES-neo.WPRE (MT130)
(Plasmid #145843)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 145843 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    RRL.sin.cPPT.SFFV/IRES-neo.WPRE.
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    human TMPRSS2 variant 1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1590
  • Mutation
    G8V
  • Entrez Gene
    TMPRSS2 (a.k.a. PRSS10)
  • Promoter SFFV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (unknown if destroyed)
  • 3′ cloning site XhoI (unknown if destroyed)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    lentiviral puroR: puroR replaced by neoR RRL.sin.cPPT.SFFV/IRES-neo.WPRE
  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry

Depositor Comments

NM_001135099 with 1 mutation G8V

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    RRL.sin.cPPT.SFFV/TMPRSS2 (variant 1).IRES-neo.WPRE (MT130) was a gift from Caroline Goujon (Addgene plasmid # 145843 ; http://n2t.net/addgene:145843 ; RRID:Addgene_145843)
  • For your References section:

    SARS-CoV-2 triggers an MDA-5-dependent interferon response which is unable to control replication in lung epithelial cells. Rebendenne A, Valadao ALC, Tauziet M, Maarifi G, Bonaventure B, McKellar J, Planes R, Nisole S, Arnaud-Arnould M, Moncorge O, Goujon C. J Virol. 2021 Jan 29. pii: JVI.02415-20. doi: 10.1128/JVI.02415-20. 10.1128/JVI.02415-20 PubMed 33514628