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Comparative optimization of combinatorial CRISPR screens.
Li R, Klingbeil O, Monducci D, Young MJ, Rodriguez DJ, Bayyat Z, Dempster JM, Kesar D, Yang X, Zamanighomi M, Vakoc CR, Ito T, Sellers WR
Nat Commun. 2022 May 5;13(1):2469. doi: 10.1038/s41467-022-30196-9.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
192205pWRS_1001lentiviral expression of SpCas9 and two pol III promoters for dual expression of sgRNAs
192206pWRS3001_VCR1_WCR3VCR1 and WCR3 tracrRNAs cloned into pTWIST AMP high copy backbone

Recombinant Antibodies from Article