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Enhanced prime editing systems by manipulating cellular determinants of editing outcomes.
Chen PJ, Hussmann JA, Yan J, Knipping F, Ravisankar P, Chen PF, Chen C, Nelson JW, Newby GA, Sahin M, Osborn MJ, Weissman JS, Adamson B, Liu DR
Cell. 2021 Oct 9. pii: S0092-8674(21)01065-5. doi: 10.1016/j.cell.2021.09.018.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
174817pCMV-SaPE2Mammalian expression of SaCas9 prime editor 2
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174818pCMV-SaPE2-P2A-BSDMammalian expression of SaCas9 prime editor 2 with P2A-BSD marker
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174819pCMV-PE2-P2A-BSDMammalian expression of SpCas9 prime editor 2 with P2A-BSD marker
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174820pCMV-PEmaxMammalian expression of SpCas9 PEmax prime editor
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174821pCMV-PEmax-P2A-BSDMammalian expression of SpCas9 PEmax prime editor with P2A-BSD marker
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174823pEF1a-hMLH1dn (og codon)Mammalian expression of human MLH1dn (original codon usage)
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174824pEF1a-hMLH1dnMammalian expression of human MLH1dn (codon optimized)
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174825pEF1a-mMLH1dnMammalian expression of mouse MLH1dn (codon optimized)
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174826pEF1a-hMLH1NTD-NLSMammalian expression of human MLH1NTD-NLS (codon optimized)
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174827pCMV-PE2-P2A-hMLH1dnMammalian expression of SpCas9 prime editor 2 with P2A-human MLH1dn (codon optimized)
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174828pCMV-PEmax-P2A-hMLH1dnMammalian expression of SpCas9 PEmax prime editor with P2A-human MLH1dn (codon optimized)
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174829pPC1000Lentiviral Repair-seq vector containing CRISPRi sgRNA and SaPE2 prime edit site
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178113pT7-PEmax for IVTTemplate for in vitro transcription of PEmax
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178114pT7-hMLH1dn for IVTTemplate for in vitro transcription of human MLH1dn
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