Enhanced prime editing systems by manipulating cellular determinants of editing outcomes.
Chen PJ, Hussmann JA, Yan J, Knipping F, Ravisankar P, Chen PF, Chen C, Nelson JW, Newby GA, Sahin M, Osborn MJ, Weissman JS, Adamson B, Liu DR
Cell. 2021 Oct 28;184(22):5635-5652.e29. doi: 10.1016/j.cell.2021.09.018.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 174817 | pCMV-SaPE2 | Mammalian expression of SaCas9 prime editor 2 |
| 174818 | pCMV-SaPE2-P2A-BSD | Mammalian expression of SaCas9 prime editor 2 with P2A-BSD marker |
| 174819 | pCMV-PE2-P2A-BSD | Mammalian expression of SpCas9 prime editor 2 with P2A-BSD marker |
| 174820 | pCMV-PEmax | Mammalian expression of SpCas9 PEmax prime editor |
| 174821 | pCMV-PEmax-P2A-BSD | Mammalian expression of SpCas9 PEmax prime editor with P2A-BSD marker |
| 174823 | pEF1a-hMLH1dn (og codon) | Mammalian expression of human MLH1dn (original codon usage) |
| 174824 | pEF1a-hMLH1dn | Mammalian expression of human MLH1dn (codon optimized) |
| 174825 | pEF1a-mMLH1dn | Mammalian expression of mouse MLH1dn (codon optimized) |
| 174826 | pEF1a-hMLH1NTD-NLS | Mammalian expression of human MLH1NTD-NLS (codon optimized) |
| 174827 | pCMV-PE2-P2A-hMLH1dn | Mammalian expression of SpCas9 prime editor 2 with P2A-human MLH1dn (codon optimized) |
| 174828 | pCMV-PEmax-P2A-hMLH1dn | Mammalian expression of SpCas9 PEmax prime editor with P2A-human MLH1dn (codon optimized) |
| 174829 | pPC1000 | Lentiviral Repair-seq vector containing CRISPRi sgRNA and SaPE2 prime edit site |
| 177670 | DNA repair library with 1,513 sgRNAs | CRISPR-interference (CRISPRi) library designed to target 476 genes involved in DNA repair and associated processes. It contains 1,513 sgRNAs and 60 non-targeting control sgRNAs. |
| 178113 | pT7-PEmax for IVT | Template for in vitro transcription of PEmax |
| 178114 | pT7-hMLH1dn for IVT | Template for in vitro transcription of human MLH1dn |
| 180020 | pCMV-PEmax-P2A-GFP | Mammalian expression of SpCas9 PEmax prime editor with P2A-EGFP marker |