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Rational design of highly active sgRNAs for CRISPR-Cas9-mediated gene inactivation.
Doench JG, Hartenian E, Graham DB, Tothova Z, Hegde M, Smith I, Sullender M, Ebert BL, Xavier RJ, Root DE
Nat Biotechnol. 2014 Sep 3. doi: 10.1038/nbt.3026.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
59702pXPR_011This lentiviral vector can be used to assay Cas9 activity.
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96924pLX_311-Cas9for Cas9 knockout, lentiviral expression of Cas9. Alternate plasmid name: pXR111
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Antibodies from Article