Rational design of highly active sgRNAs for CRISPR-Cas9-mediated gene inactivation.

Doench JG, Hartenian E, Graham DB, Tothova Z, Hegde M, Smith I, Sullender M, Ebert BL, Xavier RJ, Root DE
Nat Biotechnol. 2014 Sep 3. doi: 10.1038/nbt.3026. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
59702	pXPR_011	This lentiviral vector can be used to assay Cas9 activity.
96924	pLX_311-Cas9	for Cas9 knockout, lentiviral expression of Cas9. Alternate plasmid name: pXR111