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Optimized libraries for CRISPR-Cas9 genetic screens with multiple modalities.
Sanson KR, Hanna RE, Hegde M, Donovan KF, Strand C, Sullender ME, Vaimberg EW, Goodale A, Root DE, Piccioni F, Doench JG
Nat Commun. 2018 Dec 21;9(1):5416. doi: 10.1038/s41467-018-07901-8.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
92379Human CRISPRa sgRNA library Calabrese in backbone XPR_502 (P65 HSF), Set ACRISPRa
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92380Human CRISPRa sgRNA library Calabrese in backbone XPR_502 (P65 HSF), Set BCRISPRa
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92383Mouse CRISPRa sgRNA library Caprano in backbone XPR_502 (P65 HSF), Set ACRISPRa
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92384Mouse CRISPRa sgRNA library Caprano in backbone XPR_502 (P65 HSF), Set BCRISPRa
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92385Human CRISPRi sgRNA library Dolcetto in backbone XPR_050, Set ACrispri
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92386Human CRISPRi sgRNA library Dolcetto in backbone XPR_050, Set BCrispri
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96922pXPR_501for CRISPRa, lentiviral expression of gRNA scaffold using activator VP64
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96923pXPR_502for CRISPRa, lentiviral expression of gRNA scaffold using activator P65 HSF
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96925pXPR_050lentiviral expression of gRNA scaffold
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104090Mouse CRISPRi sgRNA library Dolomiti in backbone XPR_050, Set ACrispri
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104091Mouse CRISPRi sgRNA library Dolomiti in backbone XPR_050, Set BCrispri
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