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PurposeFor vesicle exocytosis visualization using pHmScarlet
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 166890 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backboneEGFP-N1
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Modifications to backboneEGFP was replaced with pHmScarlet
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameVAMP2
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SpeciesM. musculus (mouse)
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Entrez GeneVamp2 (a.k.a. Syb-2, Syb2, Vamp-2, sybII)
- Promoter CMV
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Tag
/ Fusion Protein
- pHmScarlet (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
- 3′ cloning site Unknown (unknown if destroyed)
- 5′ sequencing primer Unknown (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
VAMP2-pHmScarlet was a gift from Pingyong Xu (Addgene plasmid # 166890 ; http://n2t.net/addgene:166890 ; RRID:Addgene_166890) -
For your References section:
pHmScarlet is a pH-sensitive red fluorescent protein to monitor exocytosis docking and fusion steps. Liu A, Huang X, He W, Xue F, Yang Y, Liu J, Chen L, Yuan L, Xu P. Nat Commun. 2021 Mar 3;12(1):1413. doi: 10.1038/s41467-021-21666-7. 10.1038/s41467-021-21666-7 PubMed 33658493
