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Rescue of Fragile X Syndrome Neurons by DNA Methylation Editing of the FMR1 Gene.
Liu XS, Wu H, Krzisch M, Wu X, Graef J, Muffat J, Hnisz D, Li CH, Yuan B, Xu C, Li Y, Vershkov D, Cacace A, Young RA, Jaenisch R
Cell. 2018 Feb 8. pii: S0092-8674(18)30049-7. doi: 10.1016/j.cell.2018.01.012.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
108245Fuw-dCas9-Tet1CD-P2A-BFPLentiviral vector to express dCas9-Tet1CD-P2A-tagBFP
108246Fuw-dCas9-dead Tet1CD-P2A-BFPLentiviral vector to express dCas9-dead Tet1CD-P2A-tagBFP
108247Fuw-AcrIIA4-P2A-GFPLentiviral vector to express AcrIIA4-P2A-GFP
108248pgRNA-CGGLentiviral vector to express sgRNA targeting "GGCGGCGGCGGCGGCGGCGG" locus by SpCas9

Recombinant Antibodies from Article