Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

Fuw-dCas9-Tet1CD-P2A-BFP
(Plasmid #108245)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 108245 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    Fuw
  • Backbone size w/o insert (bp) 9216
  • Total vector size (bp) 16359
  • Modifications to backbone
    Fuw-AscI-EcoRI-HA-P2A-tagBFP
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Zeo marker is outside the LTRs and will not be packaged into virus

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    dCas9-Tet1CD-HA-P2A-BFP
  • Species
    Synthetic
  • Insert Size (bp)
    7143
  • Mutation
    dCas9-D10A, dCas9-H840A
  • Promoter Ubc
  • Tag / Fusion Protein
    • HA

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AscI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer aactatgcgctcggg
  • 3′ sequencing primer taaagcagcgtatcc
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Fuw-dCas9-Tet1CD-P2A-BFP was a gift from Rudolf Jaenisch (Addgene plasmid # 108245 ; http://n2t.net/addgene:108245 ; RRID:Addgene_108245)
  • For your References section:

    Rescue of Fragile X Syndrome Neurons by DNA Methylation Editing of the FMR1 Gene. Liu XS, Wu H, Krzisch M, Wu X, Graef J, Muffat J, Hnisz D, Li CH, Yuan B, Xu C, Li Y, Vershkov D, Cacace A, Young RA, Jaenisch R. Cell. 2018 Feb 8. pii: S0092-8674(18)30049-7. doi: 10.1016/j.cell.2018.01.012. 10.1016/j.cell.2018.01.012 PubMed 29456084