pET15b-AavLEA1
(Plasmid #53093)

• Purpose: AavLEA1 expression (in BL21 DE3 E. coli strain)
• Depositing Lab: Claude Férec
• Publication: Tripathi et al PLoS One. 2014 Aug 13;9(8):e104970. doi: 10.1371/journal.pone.0104970. eCollection 2014.

Backbone

• Vector backbone: pET-15b
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 5708
• Total vector size (bp): 6140
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: DH5alpha is used for growth and propagation, but protein expression requires strain BL21 DE3
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: AavLEA1
• Alt name: LEA1
• Alt name: Late embryogenesis abundant protein 1
• Species: Aphelenchus avenae
• Insert Size (bp): 432
• GenBank ID: AF423069.1 AAL18843.1
• Promoter: T7
• Tag / Fusion Protein:
  • 6xHis (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: pBRrevBam; T7
• 3′ sequencing primer: T7term

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Bridget Culleton, NUI Maynooth, Ireland
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry